Journal ArticleDOI
Serodiagnosis of Burkholderia mallei infections in horses: state-of-the-art and perspectives.
Heinrich Karl Johann Neubauer,Lisa D. Sprague,R. Zacharia,Herbert Tomaso,S. Al Dahouk,Renate Wernery,Ulrich Wernery,Holger C. Scholz +7 more
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TLDR
Future perspectives for the development and evaluation of serological test kits using well-characterized single antigens are discussed in the light of recent molecular research on B. mallei and the closely related saprozoonotic agent B. pseudomallei.Abstract:
Burkholderia mallei causes glanders or farcy in solipeds, a disease that must be reported to the OIE (Office International des Epizooties, Paris, France). The number of reported outbreaks has increased steadily during the last decade. Serodiagnosis is hampered by the considerable number of false-positives and -negatives of the internationally prescribed tests. The major problem leading to low sensitivity and specificity of complement fixation test (CFT) and enzyme-linked immunosorbent assay (ELISA) has been linked to the test antigens currently used, i.e. crude preparations of whole cells. Future perspectives for the development and evaluation of serological test kits using well-characterized single antigens are discussed in the light of recent molecular research on B. mallei and the closely related saprozoonotic agent B. pseudomallei.read more
Citations
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Glanders: off to the races with Burkholderia mallei
TL;DR: This minireview will focus on current efforts to elucidate B. mallei virulence, the associated host immune responses elicited during infection and discuss the feasibility of vaccine development.
Journal ArticleDOI
Glanders in Animals: A Review on Epidemiology, Clinical Presentation, Diagnosis and Countermeasures
Iahtasham Khan,Lothar H. Wieler,Falk Melzer,Mandy C. Elschner,Ghulam Muhammad,Shahzad Ali,Lisa D. Sprague,Heinrich Karl Johann Neubauer,Muhammad Saqib +8 more
TL;DR: Although glanders has been eradicated from most countries, it has regained the status of a re-emerging disease because of the numerous recent outbreaks and combined use of both serological and molecular detection methods increases the detection rate of glanders.
Journal ArticleDOI
Glanders: an overview of infection in humans
TL;DR: A literature review of human glanders is presented in which it is discussed the clinical epidemiology and risk factors, potential routes of exposure, symptoms, the incubation period, and specific diagnostics, and a clinical definition of human pulmonary glanders infection is proposed.
Journal ArticleDOI
An allelic exchange system for compliant genetic manipulation of the select agents Burkholderia pseudomallei and Burkholderia mallei.
TL;DR: The suicide plasmid pMo130 is developed, which allows for the compliant genetic manipulation of the select agents B. pseudomallei and B. mallei using allelic exchange and significantly expands the number of available tools that are select-agent compliant for the genetic manipulation in Burkholderia.
References
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Journal ArticleDOI
Proposal of Burkholderia gen. nov. and Transfer of Seven Species of the Genus Pseudomonas Homology Group II to the New Genus, with the Type Species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov.
Eiko Yabuuchi,Yoshimasa Kosako,Hiroshi Oyaizu,Ikuya Yano,Hisako Hotta,Yasuhiro Hashimoto,Takayuki Ezaki,Michio Arakawa +7 more
TL;DR: A new genus Burkholderia is proposed for the RNA homology group II of genus Pseudomonas and seven species in this group were transfered to the new genus.
Journal ArticleDOI
Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei
Matthew T. G. Holden,Richard W. Titball,Richard W. Titball,Sharon J. Peacock,Sharon J. Peacock,Ana Cerdeño-Tárraga,Timothy P. Atkins,Lisa Crossman,Tyrone Pitt,Carol Churcher,Karen Mungall,Stephen D. Bentley,Mohammed Sebaihia,Nicholas R. Thomson,Nathalie Bason,Ifor R. Beacham,Karen Brooks,Katherine A. Brown,Nat F. Brown,Greg L. Challis,Inna Cherevach,Tracy Chillingworth,Ann Cronin,Ben Crossett,Paul Davis,David DeShazer,Theresa Feltwell,Audrey Fraser,Zahra Hance,Heidi Hauser,S. Holroyd,Kay Jagels,Karen E. Keith,Mark Maddison,Sharon Moule,Claire Price,Michael A. Quail,Ester Rabbinowitsch,Kim Rutherford,Mandy Sanders,Mark Simmonds,Sirirurg Songsivilai,K. Stevens,Sarinna Tumapa,Monkgol Vesaratchavest,Sally Whitehead,Corin Yeats,Bart Barrell,Petra C. F. Oyston,Julian Parkhill +49 more
TL;DR: It is proposed that variable horizontal gene acquisition by B. pseudomallei is an important feature of recent genetic evolution and that this has resulted in a genetically diverse pathogenic species.
Journal ArticleDOI
Structural flexibility in the Burkholderia mallei genome
William C. Nierman,David DeShazer,H. Stanley Kim,Hervé Tettelin,Karen E. Nelson,Tamara Feldblyum,Ricky L. Ulrich,Catherine M. Ronning,Lauren M. Brinkac,Sean C. Daugherty,Tanja Davidsen,Robert T. DeBoy,George Dimitrov,Robert J. Dodson,A. Scott Durkin,Michelle L. Gwinn,Daniel H. Haft,Hoda Khouri,James F. Kolonay,Ramana Madupu,Yasmin Mohammoud,William C. Nelson,Diana Radune,Claudia M. Romero,Saul H Sarria,Jeremy D. Selengut,Christine Shamblin,Steven A. Sullivan,Owen White,Yan Yu,Nikhat Zafar,Liwei Zhou,Claire M. Fraser,Claire M. Fraser +33 more
TL;DR: Variation in simple sequence repeats in key genes can provide a mechanism for generating antigenic variation that may account for the mammalian host's inability to mount a durable adaptive immune response to a B. mallei infection.
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Detection of bacterial virulence genes by subtractive hybridization : identification of capsular polysaccharide of Burkholderia pseudomallei as a major virulence determinant
TL;DR: It was determined by enzyme-linked immunosorbent assay and immunoelectron microscopy that the inactivated gene was involved in the production of a major surface polysaccharide and facilitated the identification of an important virulence determinant in B. pseudomallei.
Journal ArticleDOI
The type II O-antigenic polysaccharide moiety of Burkholderia pseudomallei lipopolysaccharide is required for serum resistance and virulence
TL;DR: It is demonstrated that type II O‐PS is essential for B. pseudomallei serum resistance and virulence.