Journal ArticleDOI
Single step multiplex real-time RT-PCR for H5N1 influenza A virus detection.
Sunchai Payungporn,Salin Chutinimitkul,Arunee Chaisingh,Sudarat Damrongwantanapokin,Chantanee Buranathai,Alongkorn Amonsin,Apiradee Theamboonlers,Yong Poovorawan +7 more
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TLDR
The results showed that the multiplex real-time RT-PCR assays can be applied to detect virus suspensions of H5N1 influenza A virus from a wide host range and demonstrated the sensitivity of the assay amounted to approximately 10(2)-10(3)copies/mul.About:
This article is published in Journal of Virological Methods.The article was published on 2006-02-01. It has received 182 citations till now. The article focuses on the topics: Influenza A virus & Orthomyxoviridae.read more
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A review of RT-PCR technologies used in veterinary virology and disease control: sensitive and specific diagnosis of five livestock diseases notifiable to the World Organisation for Animal Health.
Bernd Hoffmann,Martin Beer,S. M. Reid,Peter P. C. Mertens,C.A.L. Oura,P.A. van Rijn,Marek J. Slomka,Jill Banks,Ian H. Brown,Dennis J. Alexander,Donald P. King +10 more
TL;DR: The rRT-PCR assays that have been developed for the detection of five RNA viruses that cause diseases that are notifiable to the World Organisation for Animal Health (OIE), namely: foot-and-mouth disease, classical swine fever, bluetongue disease, avian influenza and Newcastle disease are reviewed.
Journal ArticleDOI
An Alternative Medical Diagnosis Method: Biosensors for Virus Detection.
TL;DR: The principle and types of biosensors and their applications in the diagnosis of distinct infectious diseases were comprehensively explained and the pros and cons of existing methods as a conclusion and future perspective were elaborated.
Journal ArticleDOI
Development and Validation of a One-Step Real-Time PCR Assay for Simultaneous Detection of Subtype H5, H7, and H9 Avian Influenza Viruses
Isabella Monne,Silvia Ormelli,Annalisa Salviato,Cristian De Battisti,Francesca Bettini,Angela Salomoni,A. Drago,Bianca Zecchin,Ilaria Capua,Giovanni Cattoli +9 more
TL;DR: This is the first real-time PCR protocol available for the simultaneous detection of AI viruses belonging to subtypes H5, H7, and H9, and the results obtained indicate that this method is suitable as a routine laboratory test for the rapid detection and differentiation of the three most-important AI virus subtypes in samples of avian origin.
Journal ArticleDOI
Giant magnetoresistance-based biosensor for detection of influenza A virus
TL;DR: This study showed that the GMR biosensor assay is relevant for diagnostic application since the virus concentration in nasal samples of influenza virus infected swine was reported to be in the range of 103 to 105 TCID50/mL.
Journal ArticleDOI
A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1.
Dujuan Li,Jianping Wang,Ronghui Wang,Yanbin Li,Daad Abi-Ghanem,Luc Berghman,Billy M. Hargis,Huaguang Lu +7 more
TL;DR: It is demonstrated that the magnetic nanobeads amplification based QCM immunosensor has a great potential to be an alternative method for rapid, sensitive, and specific detection of AI virus H5N1 in agricultural, food, environmental and clinical samples.
References
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Journal ArticleDOI
Characterization of a Novel Influenza A Virus Hemagglutinin Subtype (H16) Obtained from Black-Headed Gulls
Ron A. M. Fouchier,Vincent J. Munster,Anders Wallensten,Theo M. Bestebroer,Sander Herfst,Derek J. Smith,Derek J. Smith,Gus F. Rimmelzwaan,Bjorn R. Olsen,Albert D. M. E. Osterhaus +9 more
TL;DR: A previously unidentified antigenic subtype of HA (H16), detected in viruses circulating in black-headed gulls in Sweden, is described and proposed that sequence analyses of HA and NA genes of influenza A viruses be used for the rapid identification of existing and novel HA andNA subtypes.
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The structure and receptor binding properties of the 1918 influenza hemagglutinin.
Steven J. Gamblin,Lesley F. Haire,Rupert J. Russell,D.J. Stevens,Bing Xiao,Y. Ha,N. Vasisht,David A. Steinhauer,Rod S. Daniels,Alex J. Elliot,Don C. Wiley,John J. Skehel +11 more
TL;DR: The crystal structures of the HA from the 1918 virus and two closelyrelated HAs in complex with receptor analogs are determined, explaining how the 1918 HA, while retaining receptor binding site amino acids characteristic of an avian precursor HA, is able to bind human receptors and how the virus was able to spread in the human population.
Journal ArticleDOI
Avian Influenza H5N1 in Tigers and Leopards
Juthatip Keawcharoen,Kanisak Oraveerakul,Thijs Kuiken,Ron A. M. Fouchier,Alongkorn Amonsin,Sunchai Payungporn,Suwanna Noppornpanth,Sumitra Wattanodorn,Apiradee Theamboonlers,Rachod Tantilertcharoen,Rattapan Pattanarangsan,Nlin Arya,Parntep Ratanakorn,Albert D. M. E. Osterhaus,Yong Poovorawan +14 more
TL;DR: It is demonstrated that avian influenza A (H5N1) virus caused severe pneumonia in tigers and leopards that fed on infected poultry carcasses and has implications for influenza virus epidemiology and wildlife conservation.