Spliceosomal snRNA Epitranscriptomics.
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TLDR
A review of the current knowledge of the mechanisms and functions of snRNA modifications and their biological relevance in the splicing process can be found in this paper, where the authors also discuss the role of base-pairing interactions between snRNAs and pre-mRNA.Abstract:
Small nuclear RNAs (snRNAs) are critical components of the spliceosome that catalyze the splicing of pre-mRNA. snRNAs are each complexed with many proteins to form RNA-protein complexes, termed as small nuclear ribonucleoproteins (snRNPs), in the cell nucleus. snRNPs participate in pre-mRNA splicing by recognizing the critical sequence elements present in the introns, thereby forming active spliceosomes. The recognition is achieved primarily by base-pairing interactions (or nucleotide-nucleotide contact) between snRNAs and pre-mRNA. Notably, snRNAs are extensively modified with different RNA modifications, which confer unique properties to the RNAs. Here, we review the current knowledge of the mechanisms and functions of snRNA modifications and their biological relevance in the splicing process.read more
Citations
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References
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The Spliceosome: Design Principles of a Dynamic RNP Machine
TL;DR: The spliceosome exhibits exceptional compositional and structural dynamics that are exploited during substrate-dependent complex assembly, catalytic activation, and active site remodeling in the pre-mRNAs.
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Dynamic RNA Modifications in Gene Expression Regulation
TL;DR: Roles for mRNA modification in nearly every aspect of the mRNA life cycle, as well as in various cellular, developmental, and disease processes are revealed.
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Spliceosome structure and function.
Cindy L. Will,Reinhard Lührmann +1 more
TL;DR: The extensive interplay of RNA and proteins in aligning the pre-mRNA's reactive groups, and the presence of both RNA and protein at the core of the splicing machinery, suggest that the spliceosome is an RNP enzyme, but elucidation of the precise nature of its active site awaits the generation of a high-resolution structure of its RNP core.
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Spliced segments at the 5' terminus of adenovirus 2 late mRNA
TL;DR: Four segments of viral RNA may be joined together during the synthesis of mature hexon mRNA, a model is presented for adenovirus late mRNA synthesis that involves multiple splicing during maturation of a larger precursor nuclear RNA.
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Mechanical Devices of the Spliceosome: Motors, Clocks, Springs, and Things
TL;DR: This work aims to provide a history of canine coronavirus infection in the context of central giant cell granuloma and aims to establish a cause-and-effect relationship between infection and disease progression.