Journal ArticleDOI
Standardization of platelet-derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: a first step towards multicenter studies?
Stéphane Robert,Philippe Poncelet,Romaric Lacroix,Laurent Arnaud,L. Giraudo,A. Hauchard,José Sampol,Françoise Dignat-George +7 more
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TLDR
This calibrated‐bead strategy allowed full long‐term control of the FCM‐based microparticle protocol and reproducible PMP counts over time and opens the way for multicenter studies.About:
This article is published in Journal of Thrombosis and Haemostasis.The article was published on 2009-01-01. It has received 293 citations till now.read more
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Standardization of sample collection, isolation and analysis methods in extracellular vesicle research
Kenneth W. Witwer,Edit I. Buzás,Lynne T. Bemis,Adriana Bora,Cecilia Lässer,Jan Lötvall,Esther Nolte-‘t Hoen,Melissa G. Piper,Sarada Sivaraman,Johan Skog,Clotilde Théry,Marca H. M. Wauben,Fred H. Hochberg +12 more
TL;DR: The need for standardization of specimen handling, appropriate normative controls, and isolation and analysis techniques to facilitate comparison of results is emphasized, and it is recognized that continual development and evaluation of techniques will be necessary as new knowledge is amassed.
Journal ArticleDOI
Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles
Bence György,Tamás G. Szabó,Maria Pasztoi,Maria Pasztoi,Zsuzsanna Pál,Petra Misják,Borbala Aradi,Valéria László,Éva Pállinger,Éva Pállinger,Erna Pap,Ágnes Kittel,György Nagy,András Falus,András Falus,Edit I. Buzás +15 more
TL;DR: A comprehensive overview of extracellular vesicles is given in this article, where the authors compare results from meta-analyses of published proteomic studies on membrane Vesicles.
Journal ArticleDOI
Methodological Guidelines to Study Extracellular Vesicles.
Frank A. W. Coumans,Alain Brisson,Edit I. Buzás,Françoise Dignat-George,Esther E.E. Drees,Samir El-Andaloussi,Samir El-Andaloussi,Costanza Emanueli,Costanza Emanueli,Aleksandra Gasecka,Aleksandra Gasecka,An Hendrix,Andrew F. Hill,Romaric Lacroix,Yi Lee,Ton G. van Leeuwen,Nigel Mackman,Imre Mäger,Imre Mäger,John P. Nolan,Edwin van der Pol,D. Michiel Pegtel,Susmita Sahoo,Pia Siljander,Guus Sturk,Olivier De Wever,Rienk Nieuwland +26 more
TL;DR: This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs.
Journal ArticleDOI
Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration.
N. Arraud,Romain Linares,Sisareuth Tan,Céline Gounou,J.-M. Pasquet,Stéphane Mornet,Alain Brisson +6 more
TL;DR: In this article, an original approach based on sedimentation on electron microscopy grids was developed for enumerating membranous extracellular vesicles (EVs), which are considered to derive from activated or apoptotic cells.
Journal ArticleDOI
Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans
TL;DR: The clinical significance of biomarkers of oxidative stress in humans must come from a critical analysis of the markers that should give an overall index of redox status in particular conditions.
References
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Journal ArticleDOI
Activated platelets release two types of membrane vesicles: microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and alpha-granules.
TL;DR: Two different types of membrane vesicles are released after stimulation of platelets with thrombin receptor agonist peptide SFLLRN or -thrombin: microvesicles of 100 nm to 1 μm, and exosomes measuring 40 to 100 nm in diameter, similar in size as the internal vesicle in MVBs and -granules.
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Circulating microparticles: pathophysiology and clinical implications
TL;DR: The genesis and role of microparticles, derived from platelets, endothelial cells and monocytes, in sepsis, heparin-induced thrombocytopenia, TTP, aplastic anaemia, paroxysmal nocturnal haemoglobinuria, and sickle cell disease have been well studied, and provide important insights into the underlying diseases.
Journal ArticleDOI
Circulating Endothelial Microparticles Are Associated with Vascular Dysfunction in Patients with End-Stage Renal Failure
Nicolas Amabile,Alain P. Guerin,Aurélie S. Leroyer,Ziad Mallat,Clément Nguyen,Jacques Boddaert,Gérard M. London,Alain Tedgui,Chantal M. Boulanger +8 more
TL;DR: It is suggested that circulating microparticles of endothelial origin are tightly associated with endothelial dysfunction and arterial dysfunction in patients with ESRF.
Journal ArticleDOI
Microparticle-associated tissue factor activity: a link between cancer and thrombosis?
M. E. T. Tesselaar,Fred P.H.T.M. Romijn,I. K. Van Der Linden,F. A. Prins,R M Bertina,Susanne Osanto +5 more
TL;DR: In this article, the authors investigated the association between clinically manifest venous thromboembolism (VTE) and procoagulant properties of circulating microparticles (MP) isolated from blood of unselected pancreatic and breast adenocarcinoma patients' consecutive subjects, who presented with ultrasound or CT-scan confirmed VTE, and healthy subjects.
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Procoagulant Microparticles Disrupting the Vascular Homeostasis Equation
Olivier Morel,Florence Toti,Bénédicte Hugel,Babe Bakouboula,Laurence Camoin-Jau,Françoise Dignat-George,Jean-Marie Freyssinet +6 more
TL;DR: Pharmacological strategies aimed at modulating the release of procoagulant MPs appear a promising therapeutic approach of both thrombotic processes and bleeding disorders.