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Journal ArticleDOI

STED super-resolved microscopy.

Giuseppe Vicidomini, +3 more
- 29 Jan 2018 - 
- Vol. 15, Iss: 3, pp 173-182
TLDR
The future of this method in regard to spatiotemporal limits, live-cell imaging and combination with spectroscopy is discussed, and advances in these areas may elevate STED microscopy to a standard method for imaging in the life sciences.
Abstract: 
Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while preserving useful aspects of fluorescence microscopy, such as optical sectioning, and molecular specificity and sensitivity However, sophisticated microscopy architectures and high illumination intensities have limited STED microscopy's widespread use in the past Here we summarize the progress that is mitigating these problems and giving substantial momentum to STED microscopy applications We discuss the future of this method in regard to spatiotemporal limits, live-cell imaging and combination with spectroscopy Advances in these areas may elevate STED microscopy to a standard method for imaging in the life sciences

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Citations
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Journal ArticleDOI

Generation of extreme-ultraviolet beams with time-varying orbital angular momentum

Laura Rego, +13 more
- 28 Jun 2019 - 
TL;DR: In this article, the authors introduced a property of light beams, manifested as a temporal OAM variation along a pulse: the self-torque of light, which is found in diverse physical systems (i.e., electrodynamics and general relativity).
Journal ArticleDOI

Parameter-free image resolution estimation based on decorrelation analysis.

Adrien Descloux, +2 more
- 26 Aug 2019 - 
TL;DR: Decorrelation analysis offers an improved method for assessing image resolution that works on a single image and is insensitive to common image artifacts and can be applied generally to any type of microscopy images.
Journal ArticleDOI

A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM

Marco Castello, +16 more
- 14 Jan 2019 - 
TL;DR: An implementation of ISM based on a single-photon detector array that enables super-resolution FLIM and improves multicolor, live-cell and in-depth imaging, thereby paving the way for a massive transition from confocal microscopy to ISM.
Journal ArticleDOI

Self-Sorted, Random, and Block Supramolecular Copolymers via Sequence Controlled, Multicomponent Self-Assembly

Aritra Sarkar, +11 more
- 01 Apr 2020 - 
TL;DR: This work demonstrates unprecedented two-component sequence controlled supramolecular co-polymerization by manipulating thermodynamic and kinetic routes in the pathway complexity of self-assembly of the constitutive monomers.
Journal ArticleDOI

Fourier ring correlation simplifies image restoration in fluorescence microscopy.

Sami Koho, +7 more
- 15 Jul 2019 - 
TL;DR: It is demonstrated that FRC can be leveraged in blind image restoration methods, such as image deconvolution, and is applied to perform image de-noising by frequency domain filtering.
References
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Journal ArticleDOI

Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy

Stefan W. Hell, +1 more
- 01 Jun 1994 - 
TL;DR: A new type of scanning fluorescence microscope capable of resolving 35 nm in the far field is proposed, overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation point-spread function.
Journal Article

Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy

Stefan W. Hell, +1 more
- 01 Jan 2003 - 
TL;DR: In this paper, the authors proposed a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation point spread function.
Journal ArticleDOI

Far-Field Optical Nanoscopy

Stefan W. Hell
- 25 May 2007 - 
TL;DR: Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.
Journal ArticleDOI

Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission.

Thomas A. Klar, +4 more
- 18 Jul 2000 - 
TL;DR: The diffraction barrier responsible for a finite focal spot size and limited resolution in far-field fluorescence microscopy has been fundamentally broken by quenching excited organic molecules at the rim of the focal spot through stimulated emission.
Journal ArticleDOI

Lattice Light Sheet Microscopy: Imaging Molecules to Embryos at High Spatiotemporal Resolution

Bi-Chang Chen, +27 more
- 24 Oct 2014 - 
TL;DR: A new microscope using ultrathin light sheets derived from two-dimensional optical lattices is developed, demonstrating the performance advantages of lattice light-sheet microscopy compared with previous techniques and highlighted phenomena that, when seen at increased spatiotemporal detail, may hint at previously unknown biological mechanisms.
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