Journal ArticleDOI
Template-determined, variable rate of RNA chain elongation
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TLDR
Nucleotide sequence analysis of the most prominent elongation intermediates indicated that they all have the potential to form a 3' terminal hairpin structure, which suggests that the marked variability in the rate of chain elongation is due to the formation of terminal hairpins in the product strand, or the reformation of hairpin in the template strand.About:
This article is published in Cell.The article was published on 1978-10-01. It has received 113 citations till now. The article focuses on the topics: Coding strand & RNA-dependent RNA polymerase.read more
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tRNA punctuation model of RNA processing in human mitochondria
TL;DR: It is proposed that the H strand is transcribed into a single polycistronic RNA molecule, which is processed later into mature species by precise endonucleolytic cleavages which occur, in most cases, immediately before and after a tRNA sequence.
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RNA recombination in animal and plant viruses.
TL;DR: The high frequency and widespread nature of RNA recombination indicate that this phenomenon plays a more significant role in the biology of RNA viruses than was previously recognized.
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The quasispecies (extremely heterogeneous) nature of viral RNA genome populations: biological relevance — a review
Esteban Domingo,Encarnación Martínez-Salas,Francisco Sobrino,Juan Carlos de la Torre,Agustín Portela,Juan Ortín,Cecilio López-Galíndez,Pilar Pérez-Breña,Nieves Villanueva,Rafael Nájera,Scott VandePol,David A. Steinhauer,Nicholas J. Depolo,John J. Holland +13 more
TL;DR: Evidence that cloned (or uncloned) populations of most RNA viruses do not consist of a single genome species of defined sequence, but rather of heterogeneous mixtures of related genomes (quasispecies) is reviewed.
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New insights into the mechanisms of RNA recombination.
Peter D. Nagy,Anne E. Simon +1 more
TL;DR: The most accepted models of recombination are related phenomena, including mutagenesis and nonrethe replicase-driven template switching model, the RNA combination-based genome repair, and the breakage-induced early studies identified RNA recombination events intemplate switching model as discussed by the authors.
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Exponential Amplification of Recombinant- RNA Hybridization Probes
TL;DR: The synthesized recombinant RNA molecules that function both as hybridization probes and as templates for exponential amplification by Qβ replicase demonstrate the feasibility of employing exponentially replicatable RNAs in bioassays, where they serve the dual role of specific probe and amplifiable reporter.
References
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A two-dimensional fractionation procedure for radioactive nucleotides
TL;DR: High-voltage ionophoresis is used in both dimensions for the two-dimensional fractionation of ribonuclease digests of 32P-labelled RNA and the determination of the sequence of a nucleotide by partial digestion with spleen phosphodiesterase.
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Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis
TL;DR: The use of polyacrylamide gel electrophoresis to estimate chain lengths of double- and single-stranded DNA molecules in the size range 20-1000 base pairs (or nucleotides) is described.
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Electrophoretic separation of viral nucleic acids on polyacrylamide gels
TL;DR: The elution of viral RNA from gel slices and the demonstration of infectivity after electrophoresis are described, showing a general relationship between the logarithm of the molecular weight and the relative electrophoretic mobility.
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The relationship between function and DNA sequence in an intercistronic regulatory region in phage λ
TL;DR: The potential molecular interactions and structural overlaps of these control signals apparently couple the regulation of the decision between lytic and lysogenic growth patterns by phage λ.
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Enzymatic in vitro synthesis of globin genes.
TL;DR: Reassociation, hybridization, and restriction endonuclease studies, as well as electrophoretic analyses, indicate that the sequential actions of reverse transcriptase, DNA polymerase 1, and S1 nuclease generate full-length, double-stranded synthetic globin genes.