The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more
TLDR
The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.About:
This article is published in Journal of Biological Chemistry.The article was published on 1964-07-01 and is currently open access. It has received 11895 citations till now. The article focuses on the topics: Carbon monoxide binding & Microsome.read more
Citations
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Book ChapterDOI
Bioconcentration, bioaccumulation, and metabolism of pesticides in aquatic organisms.
TL;DR: This chapter provides an overview of the enzymes that are capable of metabolizing or otherwise assisting in the removal of xenobiotics from aquatic species and their roles in ecotoxicological assessment.
Journal ArticleDOI
Cytochromes P-450 from cassava (Manihot esculenta Crantz) catalyzing the first steps in the biosynthesis of the cyanogenic glucosides linamarin and lotaustralin. Cloning, functional expression in Pichia pastoris, and substrate specificity of the isolated recombinant enzymes.
TL;DR: Reconstitution in lipid micelles showed that CYP79D1 has a higher k(c) value with L-valine as substrate than withL-isoleucine, which is consistent with linamarin being the major cyanogenic glucoside in cassava.
Journal ArticleDOI
On the inhibitory action of mersalyl on microsomal drug oxidation: a rigid organization of the electron transport chain.
TL;DR: Studies on the pattern of inhibition suggest a rigid electron-transport system linking a single molecule of TPNH-cytochrome c reductase to a number of molecules of cytochrome P-450 contained within a multicomponent complex and that reducing equivalents can be transferred only within a single enzyme complex with no demonstrable electron transfer interactions between the components of different complexes.
Journal ArticleDOI
CYP-specific bioactivation of four organophosphorothioate pesticides by human liver microsomes.
TL;DR: The data indicated that CYP1A2 and 2B6 are suggested as possible metabolic biomarkers of susceptibility to OPT toxic effect at the actual human exposure levels, while the role of CYP3A4 is more significant to the low-affinity component of OPT bioactivation.
Journal Article
Identification of cytochrome P-450 isoforms responsible for cis-tramadol metabolism in human liver microsomes.
Vangala V. Subrahmanyam,A.B. Renwick,David G. Walters,Philip J. Young,R.J. Price,Alfred Tonelli,Brian G. Lake +6 more
TL;DR: It is demonstrated that cis-tramadol can be metabolized to tramadol metabolites M1, M2, M3, and M5 in human liver microsomal preparations.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The Carbon Monoxide-binding Pigment of Liver Microsomes II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES
Tsuneo Omura,Ryo Sato +1 more
TL;DR: The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.
Journal ArticleDOI
Hepatic Triphosphopyridine Nucleotide-Cytochrome c Reductase: Isolation, Characterization, and Kinetic Studies
TL;DR: Evidence is presented which suggests that this enzyme participates in a microsomal elect,ron transport system which does not include cytochrome c and kinetic evidence has been obtained which allows certain conclusions to be drawn concerning the mechanism of catalysis by this enzyme.
Journal ArticleDOI
Microsomal triphosphopyridine nucleotide-cytochrome c reductase of liver.
Charles Williams,Henry Kamin +1 more
TL;DR: The biological role of reduced triphosphopyridine nucleotide (TPNH) and the metabolic pathways of its hydrogen atom and electron appear to be fundamentally different from those of reduced diphosphipyridineucleotide (DPNH).
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The Carbon Monoxide-binding Pigment of Liver Microsomes II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES
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