The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more
TLDR
The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.About:
This article is published in Journal of Biological Chemistry.The article was published on 1964-07-01 and is currently open access. It has received 11895 citations till now. The article focuses on the topics: Carbon monoxide binding & Microsome.read more
Citations
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The effects of buthionine sulphoximine (BSO) on glutathione depletion and xenobiotic biotransformation
Roger Drew,John O. Miners +1 more
TL;DR: Since BSO does not affect cytochrome P-450 or conjugating enzyme activity, its use as a specific depletor of tissue GSH in the investigation of mechanisms of xenobiotic-induced toxicities is preferable to the standard GSH-depleting agents as these have other enzyme effects.
Journal Article
A Method for the Simultaneous Evaluation of the Activities of Seven Major Human Drug-Metabolizing Cytochrome P450s Using an in Vitro Cocktail of Probe Substrates and Fast Gradient Liquid Chromatography Tandem Mass Spectrometry
Elizabeth A. Dierks,Karen R. Stams,Heng-Keang Lim,Georgia Cornelius,Honglu Zhang,Simon Edward Ball +5 more
TL;DR: This cocktail method offers an efficient, robust way to determine the cytochrome P450 inhibition profile of large numbers of compounds and greatly facilitates its use to assess CYP inhibition as a drug candidate selection criterion.
Journal ArticleDOI
Expression of rat liver cytochrome P-450MC cDNA in Saccharomyces cerevisiae.
TL;DR: Rat liver cytochrome P-450MC cDNA was inserted between the ADH1 promoter and terminator regions of the yeast expression vector pAAH5 and on introduction of the resulting recombinant plasmid pAMC1, Saccharomyces cerevisiae cells synthesized up to 8 X 10(5) molecules per cell of the cyto Chrome protein, most of which was localized in yeast microsomes.
Journal ArticleDOI
Cytochrome P450 monooxygenases and insecticide resistance in insects.
TL;DR: In Drosophila melanogaster, it is shown that the overproduction of cytochrome P450 can be lost by the fly without a corresponding complete loss of resistance, which prompted the sequencing of a cyto chrome P450 candidate for resistance in resistant and susceptible flies.
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Cytochrome P450 enzymes in aquatic invertebrates: recent advances and future directions
TL;DR: Molecular biology tools have greatly aided the search for P450 gene diversity in aquatic invertebrates, and are likely to identify as much diversity for this protein superfamily as is present in higher marine and terrestrial organisms.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The Carbon Monoxide-binding Pigment of Liver Microsomes II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES
Tsuneo Omura,Ryo Sato +1 more
TL;DR: The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.
Journal ArticleDOI
Hepatic Triphosphopyridine Nucleotide-Cytochrome c Reductase: Isolation, Characterization, and Kinetic Studies
TL;DR: Evidence is presented which suggests that this enzyme participates in a microsomal elect,ron transport system which does not include cytochrome c and kinetic evidence has been obtained which allows certain conclusions to be drawn concerning the mechanism of catalysis by this enzyme.
Journal ArticleDOI
Microsomal triphosphopyridine nucleotide-cytochrome c reductase of liver.
Charles Williams,Henry Kamin +1 more
TL;DR: The biological role of reduced triphosphopyridine nucleotide (TPNH) and the metabolic pathways of its hydrogen atom and electron appear to be fundamentally different from those of reduced diphosphipyridineucleotide (DPNH).
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The Carbon Monoxide-binding Pigment of Liver Microsomes II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES
Tsuneo Omura,Ryo Sato +1 more