The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more
TLDR
The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.About:
This article is published in Journal of Biological Chemistry.The article was published on 1964-07-01 and is currently open access. It has received 11895 citations till now. The article focuses on the topics: Carbon monoxide binding & Microsome.read more
Citations
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Journal ArticleDOI
Aryl acetylenes as mechanism-based inhibitors of cytochrome P450-dependent monooxygenase enzymes.
TL;DR: The suicide inhibition of P450s by propynylaryl acetylenes proceeds via a methylaryl ketene formed by a 1,2-methyl rearrangement, analogous to the mechanism of suicide inhibition by ethynylacetylenes that proceed via ketene intermediates formed by 1, 2-hydrogen shifts.
Journal ArticleDOI
Expression of human liver cytochrome P450 IIIA4 in yeast. A functional model for the hepatic enzyme.
TL;DR: Yeast microsomes containing P450 NF25 constitute by themselves a good functional model for studying the binding capacities and catalytic activities of this individual form of human hepatic cytochrome P-450, as shown by difference visible spectroscopy.
Journal ArticleDOI
Enhanced formation of rapidly labeled bilirubin by phenobarbital: Hepatic microsomal cytochromes as a possible source
Journal ArticleDOI
Sex differences in subacute toxicity and hepatic microsomal metabolism of triptolide in rats.
Li Liu,Zhenzhou Jiang,Jing Liu,Xin Huang,Tao Wang,Jun Liu,Yun Zhang,Zhixing Zhou,Jianlu Guo,Lina Yang,Yun Chen,Luyong Zhang +11 more
TL;DR: Evaluated the oral toxicity of triptolide in Sprague-Dawley rats and suggested that CYP3A2 made an important contribution to the sex-related metabolism of triPTolide, which may result in the sex differences in triptoide toxicity.
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Characterization of the alkane-inducible cytochrome P450 (P450alk) gene from the yeast Candida tropicalis: identification of a new P450 gene family.
Dominique Sanglard,John C. Loper +1 more
TL;DR: The P450alk gene, which is inducible by the assimilation of alkane in Candida tropicalis, was sequenced and characterized and was therefore the first member (A1) of a new P450 gene family (LII).
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The Carbon Monoxide-binding Pigment of Liver Microsomes II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES
Tsuneo Omura,Ryo Sato +1 more
TL;DR: The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.
Journal ArticleDOI
Hepatic Triphosphopyridine Nucleotide-Cytochrome c Reductase: Isolation, Characterization, and Kinetic Studies
TL;DR: Evidence is presented which suggests that this enzyme participates in a microsomal elect,ron transport system which does not include cytochrome c and kinetic evidence has been obtained which allows certain conclusions to be drawn concerning the mechanism of catalysis by this enzyme.
Journal ArticleDOI
Microsomal triphosphopyridine nucleotide-cytochrome c reductase of liver.
Charles Williams,Henry Kamin +1 more
TL;DR: The biological role of reduced triphosphopyridine nucleotide (TPNH) and the metabolic pathways of its hydrogen atom and electron appear to be fundamentally different from those of reduced diphosphipyridineucleotide (DPNH).
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The Carbon Monoxide-binding Pigment of Liver Microsomes II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES
Tsuneo Omura,Ryo Sato +1 more