The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more
TLDR
The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.About:
This article is published in Journal of Biological Chemistry.The article was published on 1964-07-01 and is currently open access. It has received 11895 citations till now. The article focuses on the topics: Carbon monoxide binding & Microsome.read more
Citations
More filters
Journal ArticleDOI
Metabolic activation of troglitazone: identification of a reactive metabolite and mechanisms involved.
Kan He,Rasmy Talaat,William F. Pool,Michael D. Reily,Jessica Elizabeth Reed,Alexander James Bridges,Thomas F. Woolf +6 more
TL;DR: The mechanisms for metabolic activation of TGZ may involve an ultimate reactive sulfonium ion which could be formed from an initial sulfoxide followed by a formal Pummerer rearrangement, or a C5 thiazolidinedione radical or a sulfur cation radical.
Journal ArticleDOI
Direct electron transfer between cytochrome P450scc and gold nanoparticles on screen-printed rhodium-graphite electrodes.
Victoria V. Shumyantseva,Sandro Carrara,Valter Bavastrello,D. Jason Riley,Tatiana V. Bulko,Konstantin G. Skryabin,Alexander I. Archakov,Claudio Nicolini +7 more
TL;DR: Investigation of electron transfer between cytochrome P450scc (CYP11A1) and gold nanoparticles immobilised on rhodium-graphite electrodes and the role of the nanoparticles in mediating electron transfer was verified confirms thatGold nanoparticles enhance electron transfer to the P 450scc when present on the r Rhodium- graphite electrodes.
Journal ArticleDOI
CYP701A8: A Rice ent-Kaurene Oxidase Paralog Diverted to More Specialized Diterpenoid Metabolism
TL;DR: It appears that OsKOL4 plays a role in the more specialized diterpenoid metabolism of rice, and the results provide evidence for divergence of a KO/CYP701 family member from GA biosynthesis.
Journal ArticleDOI
Comparison of caffeine metabolism by slices, microsomes and hepatocyte cultures from adult human liver.
TL;DR: Caffeine biotransformation by these three in vitro systems was low, however, metabolite formation was shown to proceed at a rate close to that calculated from in vivo caffeine elimination half-life.
Journal ArticleDOI
Generation and identification of reactive metabolites by electrochemistry and immobilized enzymes coupled on-line to liquid chromatography/mass spectrometry.
Wiebke Lohmann,Uwe Karst +1 more
TL;DR: Electrochemical reactor and horseradish peroxidase immobilized on magnetic microparticles with liquid chromatography/mass spectrometry allows the direct detection of reactive metabolites of the model compounds amodiaquine, amsacrine, and mitoxantrone and are promising tools for the identification of both reactive and stable metabolites in drug development.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The Carbon Monoxide-binding Pigment of Liver Microsomes II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES
Tsuneo Omura,Ryo Sato +1 more
TL;DR: The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.
Journal ArticleDOI
Hepatic Triphosphopyridine Nucleotide-Cytochrome c Reductase: Isolation, Characterization, and Kinetic Studies
TL;DR: Evidence is presented which suggests that this enzyme participates in a microsomal elect,ron transport system which does not include cytochrome c and kinetic evidence has been obtained which allows certain conclusions to be drawn concerning the mechanism of catalysis by this enzyme.
Journal ArticleDOI
Microsomal triphosphopyridine nucleotide-cytochrome c reductase of liver.
Charles Williams,Henry Kamin +1 more
TL;DR: The biological role of reduced triphosphopyridine nucleotide (TPNH) and the metabolic pathways of its hydrogen atom and electron appear to be fundamentally different from those of reduced diphosphipyridineucleotide (DPNH).
Related Papers (5)
The Carbon Monoxide-binding Pigment of Liver Microsomes II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES
Tsuneo Omura,Ryo Sato +1 more