The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more
TLDR
The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.About:
This article is published in Journal of Biological Chemistry.The article was published on 1964-07-01 and is currently open access. It has received 11895 citations till now. The article focuses on the topics: Carbon monoxide binding & Microsome.read more
Citations
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Mechanisms of Cytochrome P450-Catalyzed Oxidations
TL;DR: Current P450 questions under investigation include the potential role of the intermediate Compound 0 (formally FeIII-O2 -) in catalysis of some reactions, the roles of high- and low-spin forms of Compound I, the mechanism of desaturation, the role of open and closed structures of P450s inCatalysis, the extent of processivity in multi-step oxidations, and the roleof the accessory protein cytochrome b 5.
Journal ArticleDOI
Reconstituted Liver Microsomal Enzyme System That Hydroxylates Drugs, Other Foreign Compounds, and Endogenous Substrates VI. DIFFERENT SUBSTRATE SPECIFICITIES OF THE CYTOCHROME P450 FRACTIONS FROM CONTROL AND PHENOBARBITAL-TREATED RATS
Anthony Y. H. Lu,Wayne Levin,Susan B. West,M. Jacobson,Dene E. Ryan,R. Kuntzman,Allan H. Conney +6 more
TL;DR: The results suggest that the cytochrome P450 from control rats is catalytically different from the cy tochrome P 450 from phenobarbital-treated rats.
Journal ArticleDOI
Cytochrome P450: progress and predictions.
TL;DR: In this review, recent advances in knowledge of the catalytic properties, reaction mechanisms, and regulation of expression and activity of the P450 enzymes are briefly summarized.
Journal ArticleDOI
Identification of P450 enzymes involved in metabolism of verapamil in humans.
TL;DR: Veraparnil has the potential to interact with other drugs which are substrates or inducers of CYP3A and CYP1A2, and has been investigated in microsomes obtained from yeast cells which were genetically engineered for stable expression of human P450.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The Carbon Monoxide-binding Pigment of Liver Microsomes II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES
Tsuneo Omura,Ryo Sato +1 more
TL;DR: The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.
Journal ArticleDOI
Hepatic Triphosphopyridine Nucleotide-Cytochrome c Reductase: Isolation, Characterization, and Kinetic Studies
TL;DR: Evidence is presented which suggests that this enzyme participates in a microsomal elect,ron transport system which does not include cytochrome c and kinetic evidence has been obtained which allows certain conclusions to be drawn concerning the mechanism of catalysis by this enzyme.
Journal ArticleDOI
Microsomal triphosphopyridine nucleotide-cytochrome c reductase of liver.
Charles Williams,Henry Kamin +1 more
TL;DR: The biological role of reduced triphosphopyridine nucleotide (TPNH) and the metabolic pathways of its hydrogen atom and electron appear to be fundamentally different from those of reduced diphosphipyridineucleotide (DPNH).
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The Carbon Monoxide-binding Pigment of Liver Microsomes II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES
Tsuneo Omura,Ryo Sato +1 more