The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis
Bin Yu,Liu Bi,Binglian Zheng,Lijuan Ji,David Chevalier,David Chevalier,Manu Agarwal,Manu Agarwal,Vanitharani Ramachandran,Wan-Xiang Li,Thierry Lagrange,John C. Walker,Xuemei Chen +12 more
TLDR
It is proposed that DAWDLE (DDL), an FHA domain-containing protein in Arabidopsis, acts in the biogenesis of miRNAs and endogenous siRNAs by facilitating DCL1 to access or recognize pri-miRNAs.Abstract:
Proteins containing the forkhead-associated domain (FHA) are known to act in biological processes such as DNA damage repair, protein degradation, and signal transduction. Here we report that DAWDLE (DDL), an FHA domain-containing protein in Arabidopsis, acts in the biogenesis of miRNAs and endogenous siRNAs. Unlike mutants of genes known to participate in the processing of miRNA precursors, such as dcl1, hyponastic leaves1, and serrate, ddl mutants show reduced levels of pri-miRNAs as well as mature miRNAs. Promoter activity of MIR genes, however, is not affected by ddl mutations. DDL is an RNA binding protein and is able to interact with DCL1. In addition, we found that SNIP1, the human homolog of DDL, is involved in miRNA biogenesis and interacts with Drosha. Therefore, we uncovered an evolutionarily conserved factor in miRNA biogenesis. We propose that DDL participates in miRNA biogenesis by facilitating DCL1 to access or recognize pri-miRNAs.read more
Citations
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