The nuclear receptor Nr5a2 can replace Oct4 in the reprogramming of murine somatic cells to pluripotent cells.
Jian-Chien Dominic Heng,Bo Feng,Jianyong Han,Jianming Jiang,Petra Kraus,Jia-Hui Ng,Yuriy L. Orlov,Mikael Huss,Lin Yang,Thomas Lufkin,Thomas Lufkin,Bing Lim,Bing Lim,Huck-Hui Ng,Huck-Hui Ng +14 more
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TLDR
It is shown that the orphan nuclear receptor Nr5a2 (also known as Lrh-1) can replace Oct4 in the derivation of iPSCs from mouse somatic cells, and it can also enhance reprogramming efficiency.About:
This article is published in Cell Stem Cell.The article was published on 2010-02-05 and is currently open access. It has received 481 citations till now. The article focuses on the topics: Reprogramming & Induced pluripotent stem cell.read more
Citations
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Journal ArticleDOI
Induced pluripotency: history, mechanisms, and applications
TL;DR: This review summarizes the progress that has been made in the iPSC field over the last 4 years, with an emphasis on understanding the mechanisms of cellular reprogramming and its potential applications in cell therapy.
Journal ArticleDOI
A decade of transcription factor-mediated reprogramming to pluripotency
TL;DR: The mechanisms underlying transcription factor-mediated reprogramming are still poorly understood; however, several mechanistic insights have recently been obtained, making it more amenable to applications in the fields of regenerative medicine, disease modelling and drug discovery.
Journal ArticleDOI
Senescence impairs successful reprogramming to pluripotent stem cells
Ana Banito,Sheikh Tamir Rashid,Juan Carlos Acosta,Si De Li,Carlos Filipe Pereira,Imbisaat Geti,Sandra Pinho,José C. R. Silva,Véronique Azuara,Martin J. Walsh,Ludovic Vallier,Jesús Gil +11 more
TL;DR: Crucially, ablation of different senescence effectors improves the efficiency of reprogramming, suggesting novel strategies for maximizing the generation of iPS cells.
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Dedifferentiation, transdifferentiation and reprogramming: three routes to regeneration.
TL;DR: Current research aims to understand how the processes of dedifferentiation, transdifferentiation or reprogramming work and to eventually harness them for use in regenerative medicine.
Journal ArticleDOI
Dynamic and Coordinated Epigenetic Regulation of Developmental Transitions in the Cardiac Lineage
Joseph A. Wamstad,Jeffrey M. Alexander,Rebecca Truty,Avanti Shrikumar,Fugen Li,Kirsten Eilertson,Huiming Ding,John N. Wylie,Alexander R. Pico,John A. Capra,Genevieve D. Erwin,Steven J. Kattman,Gordon Keller,Deepak Srivastava,Stuart S. Levine,Katherine S. Pollard,Alisha K. Holloway,Laurie A. Boyer,Benoit G. Bruneau +18 more
TL;DR: A novel preactivation chromatin pattern at the promoters of genes associated with heart development and cardiac function is discovered and forms a basis for understanding developmentally regulated chromatin transitions during lineage commitment and the molecular etiology of congenital heart disease.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Journal ArticleDOI
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
Masato Nakagawa,Michiyo Koyanagi,Koji Tanabe,Kazutoshi Takahashi,Tomoko Ichisaka,Takashi Aoi,Keisuke Okita,Yuji Mochiduki,Nanako Takizawa,Shinya Yamanaka +9 more
TL;DR: A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.
Journal ArticleDOI
Integration of External Signaling Pathways with the Core Transcriptional Network in Embryonic Stem Cells
Xi Chen,Han Xu,Ping Yuan,Fang Fang,Fang Fang,Mikael Huss,Vinsensius B. Vega,Eleanor Wong,Yuriy L. Orlov,Weiwei Zhang,Weiwei Zhang,Jianming Jiang,Jianming Jiang,Yuin-Han Loh,Yuin-Han Loh,Hock Chuan Yeo,Zhen Xuan Yeo,Vipin Narang,Kunde R Govindarajan,Bernard Leong,Atif Shahab,Yijun Ruan,Guillaume Bourque,Wing-Kin Sung,Neil D. Clarke,Chia-Lin Wei,Huck-Hui Ng,Huck-Hui Ng +27 more
TL;DR: This study uses chromatin immunoprecipitation coupled with ultra-high-throughput DNA sequencing to map the locations of TF-binding sites and identifies important features of the transcriptional regulatory networks that define ES-cell identity.
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