The Tomato Terpene Synthase Gene Family
Vasiliki Falara,Tariq A. Akhtar,Thuong T.H. Nguyen,Eleni A. Spyropoulou,Petra M. Bleeker,Ines Schauvinhold,Yuki Matsuba,Megan E. Bonini,Anthony L. Schilmiller,Robert C. Schuurink,Eran Pichersky +10 more
TLDR
In this paper, it was shown that the tomato genome contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional in at least some organs or tissues of the plant.Abstract:
Compounds of the terpenoid class play numerous roles in the interactions of plants with their environment, such as attracting pollinators and defending the plant against pests. We show here that the genome of cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional. Of these 29 TPS genes, 26 were expressed in at least some organs or tissues of the plant. The enzymatic functions of eight of the TPS proteins were previously reported, and here we report the specific in vitro catalytic activity of 10 additional tomato terpene synthases. Many of the tomato TPS genes are found in clusters, notably on chromosomes 1, 2, 6, 8, and 10. All TPS family clades previously identified in angiosperms are also present in tomato. The largest clade of functional TPS genes found in tomato, with 12 members, is the TPS-a clade, and it appears to encode only sesquiterpene synthases, one of which is localized to the mitochondria, while the rest are likely cytosolic. A few additional sesquiterpene synthases are encoded by TPS-b clade genes. Some of the tomato sesquiterpene synthases use z,z-farnesyl diphosphate in vitro as well, or more efficiently than, the e,e-farnesyl diphosphate substrate. Genes encoding monoterpene synthases are also prevalent, and they fall into three clades: TPS-b, TPS-g, and TPS-e/f. With the exception of two enzymes involved in the synthesis of ent-kaurene, the precursor of gibberellins, no other tomato TPS genes could be demonstrated to encode diterpene synthases so far.read more
Citations
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Book ChapterDOI
Biosynthesis and biological functions of terpenoids in plants
TL;DR: This chapter gives an overview and highlights recent advances in the understanding of the organization, regulation, and diversification of core and specialized terpenoid metabolic pathways, and addresses the most important functions of volatile and nonvolatile terpenoids specialized metabolites in plants.
Journal ArticleDOI
Plant glandular trichomes as targets for breeding or engineering of resistance to herbivores
Joris J. Glas,Bernardus C. J. Schimmel,Juan M. Alba,Rocío Escobar-Bravo,Robert C. Schuurink,Merijn R. Kant +5 more
TL;DR: An overview of the metabolic diversity found within plant glandular trichomes is provided, with the emphasis on those of the Solanaceae, and of the tools available to manipulate their activities for enhancing the plant’s resistance to pests is provided.
Journal ArticleDOI
Plant terpenes: defense responses, phylogenetic analysis, regulation and clinical applications
Bharat Singh,R. A. Sharma +1 more
TL;DR: In this review paper, recent developments in the biosynthesis of terpenoids, indepth description of terpene synthases and their phylogenetic analysis, regulation of ter pene biosynthesis as well as updates of ter penes which have entered in the clinical studies are reviewed thoroughly.
Journal ArticleDOI
Plant metabolic clusters – from genetics to genomics
TL;DR: This review summarizes progress in the discovery of new plant natural product pathways and indicates that increased knowledge of the features of plant metabolic gene clusters - architecture, regulation and assembly - will be instrumental in expediting natural product discovery.
Journal ArticleDOI
Glandular trichomes: what comes after expressed sequence tags?
TL;DR: Glandular trichomes cover the surface of many plant species and exhibit tremendous diversity, be it in their shape or the compounds they secrete as discussed by the authors, which has spurred research into the biosynthesis pathways that lead to these specialized metabolites.
References
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Journal ArticleDOI
Sandalwood fragrance biosynthesis involves sesquiterpene synthases of both the terpene synthase (TPS)-a and TPS-b subfamilies, including santalene synthases.
Christopher G. Jones,Jessie Moniodis,Katherine G. Zulak,Adrian Scaffidi,Julie A. Plummer,Emilio L. Ghisalberti,Elizabeth L. Barbour,Jörg Bohlmann +7 more
TL;DR: Strict functionality and limited sequence divergence in the santalene and bisabolene synthases are in contrast to the TPS-a synthases, suggesting these compounds have played a significant role in the evolution of the Santalum genus.
Journal ArticleDOI
Identification and Regulation of TPS04/GES, an Arabidopsis Geranyllinalool Synthase Catalyzing the First Step in the Formation of the Insect-Induced Volatile C16-Homoterpene TMTT
Marco Herde,Katrin Gärtner,Tobias G. Köllner,Benjamin Fode,Wilhelm Boland,Jonathan Gershenzon,Christiane Gatz,Dorothea Tholl +7 more
TL;DR: The identification of Terpene Synthase 04 (TPS04; At1g61120) as a geranyllinalool synthase (GES) is reported, paving the ground to address the contribution of TMTT in ecological interactions and to elucidate the signaling network that regulates T MTT synthesis.
Journal ArticleDOI
Functional identification of AtTPS03 as (E)-beta-ocimene synthase: a monoterpene synthase catalyzing jasmonate- and wound-induced volatile formation in Arabidopsis thaliana.
TL;DR: In this article, the authors describe cDNA cloning and functional characterization of a gene encoding a highly specialized (E)-β-ocimene synthase, AtTPS03, from Arabidopsis thaliana (L.) Heynh.
Journal ArticleDOI
A Modular Approach for Facile Biosynthesis of Labdane-Related Diterpenes
TL;DR: A modular approach for facile biosynthesis of labdane-related diterpenes wherein base pGGxC vectors capable of introducing bacterial production of any one of the three common stereoisomers of CPP can be co-introduced with diterpene synthases that convert these CPP intermediates to specific diterPene hydrocarbon skeletal structures.
Journal ArticleDOI
Distortion of trichome morphology by the hairless mutation of tomato affects leaf surface chemistry
TL;DR: The results establish a link between the morphology and chemical composition of glandular trichomes in cultivated tomato, and show that hl-mediated changes in these leaf surface traits correlate with decreased resistance to insect herbivory.