The Tomato Terpene Synthase Gene Family
Vasiliki Falara,Tariq A. Akhtar,Thuong T.H. Nguyen,Eleni A. Spyropoulou,Petra M. Bleeker,Ines Schauvinhold,Yuki Matsuba,Megan E. Bonini,Anthony L. Schilmiller,Robert C. Schuurink,Eran Pichersky +10 more
TLDR
In this paper, it was shown that the tomato genome contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional in at least some organs or tissues of the plant.Abstract:
Compounds of the terpenoid class play numerous roles in the interactions of plants with their environment, such as attracting pollinators and defending the plant against pests. We show here that the genome of cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional. Of these 29 TPS genes, 26 were expressed in at least some organs or tissues of the plant. The enzymatic functions of eight of the TPS proteins were previously reported, and here we report the specific in vitro catalytic activity of 10 additional tomato terpene synthases. Many of the tomato TPS genes are found in clusters, notably on chromosomes 1, 2, 6, 8, and 10. All TPS family clades previously identified in angiosperms are also present in tomato. The largest clade of functional TPS genes found in tomato, with 12 members, is the TPS-a clade, and it appears to encode only sesquiterpene synthases, one of which is localized to the mitochondria, while the rest are likely cytosolic. A few additional sesquiterpene synthases are encoded by TPS-b clade genes. Some of the tomato sesquiterpene synthases use z,z-farnesyl diphosphate in vitro as well, or more efficiently than, the e,e-farnesyl diphosphate substrate. Genes encoding monoterpene synthases are also prevalent, and they fall into three clades: TPS-b, TPS-g, and TPS-e/f. With the exception of two enzymes involved in the synthesis of ent-kaurene, the precursor of gibberellins, no other tomato TPS genes could be demonstrated to encode diterpene synthases so far.read more
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SlMYC1 Regulates Type VI Glandular Trichome Formation and Terpene Biosynthesis in Tomato Glandular Cells
Jiesen Xu,Zeger Otto Van Herwijnen,Dorthe Bettina Drager,Chun Sui,Chun Sui,Michel A. Haring,Robert C. Schuurink +6 more
TL;DR: It is demonstrated that SlMYC1, a basic helix-loop-helix transcription factor, differentially regulates mono- and sesquiterpene biosynthesis in the type VI glandular trichomes of tomato leaves and stems.
Journal ArticleDOI
On the regulation and function of secondary metabolism during fruit development and ripening
TL;DR: Advances in tomato secondary metabolism will be reviewed here focusing on the use of metabolomics strategies and, where applicable, the enabling of these strategies by their coupling to information resident in the tomato genome sequence.
Journal ArticleDOI
Isoprene synthase genes form a monophyletic clade of acyclic terpene synthases in the TPS-B terpene synthase family.
TL;DR: This study identifies nine new isoprene synthases within the rosid angiosperms and documents the capacity of a myrcene synthase in Humulus lupulus to produceIsoprene.
Journal ArticleDOI
Emerging investigator series: molecular mechanisms of plant salinity stress tolerance improvement by seed priming with cerium oxide nanoparticles
Jing An,Jing An,Peiguang Hu,Fangjun Li,Honghong Wu,Yu Shen,Jason C. White,Xiaoli Tian,Zhaohu Li,Juan Pablo Giraldo +9 more
TL;DR: In this paper, the authors investigated how priming seeds with antioxidant poly(acrylic acid)-coated cerium oxide nanoparticles (PNC) impacts cotton seedling morphological, physiological, biochemical, and transcriptomic traits under salinity stress.
Journal ArticleDOI
Evolution of TPS20-related terpene synthases influences chemical diversity in the glandular trichomes of the wild tomato relative Solanum habrochaites.
TL;DR: It is revealed that the unusually high intra-specific variation of volatile terpene synthesis in glandular trichomes of S. habrochaites is due at least in part to evolution at the TPS20 locus.
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TL;DR: The draft genome of the black cottonwood tree, Populus trichocarpa, has been reported in this paper, with more than 45,000 putative protein-coding genes identified.
Journal ArticleDOI
The map-based sequence of the rice genome
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TL;DR: A map-based, finished quality sequence that covers 95% of the 389 Mb rice genome, including virtually all of the euchromatin and two complete centromeres, and finds evidence for widespread and recurrent gene transfer from the organelles to the nuclear chromosomes.