The Tomato Terpene Synthase Gene Family
Vasiliki Falara,Tariq A. Akhtar,Thuong T.H. Nguyen,Eleni A. Spyropoulou,Petra M. Bleeker,Ines Schauvinhold,Yuki Matsuba,Megan E. Bonini,Anthony L. Schilmiller,Robert C. Schuurink,Eran Pichersky +10 more
TLDR
In this paper, it was shown that the tomato genome contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional in at least some organs or tissues of the plant.Abstract:
Compounds of the terpenoid class play numerous roles in the interactions of plants with their environment, such as attracting pollinators and defending the plant against pests. We show here that the genome of cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional. Of these 29 TPS genes, 26 were expressed in at least some organs or tissues of the plant. The enzymatic functions of eight of the TPS proteins were previously reported, and here we report the specific in vitro catalytic activity of 10 additional tomato terpene synthases. Many of the tomato TPS genes are found in clusters, notably on chromosomes 1, 2, 6, 8, and 10. All TPS family clades previously identified in angiosperms are also present in tomato. The largest clade of functional TPS genes found in tomato, with 12 members, is the TPS-a clade, and it appears to encode only sesquiterpene synthases, one of which is localized to the mitochondria, while the rest are likely cytosolic. A few additional sesquiterpene synthases are encoded by TPS-b clade genes. Some of the tomato sesquiterpene synthases use z,z-farnesyl diphosphate in vitro as well, or more efficiently than, the e,e-farnesyl diphosphate substrate. Genes encoding monoterpene synthases are also prevalent, and they fall into three clades: TPS-b, TPS-g, and TPS-e/f. With the exception of two enzymes involved in the synthesis of ent-kaurene, the precursor of gibberellins, no other tomato TPS genes could be demonstrated to encode diterpene synthases so far.read more
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Journal ArticleDOI
De novo Sequencing and Analysis of Salvia hispanica Tissue-Specific Transcriptome and Identification of Genes Involved in Terpenoid Biosynthesis
TL;DR: Abscisic acid had the most pronounced effect on the expression of the TPS genes tested in this study, and provides valuable community resources for future studies aimed at improving and utilizing the beneficial constituents of this emerging healthy food source.
Journal ArticleDOI
Scenarios of Genes-to-Terpenoids Network Led to the Identification of a Novel α/β-Farnesene/β-Ocimene Synthase in Camellia sinensis.
Jieyang Jin,Zhang Shangrui,Mingyue Zhao,Tingting Jing,Na Zhang,Jingming Wang,Bin Wu,Chuankui Song +7 more
TL;DR: The use of an integrated approach combining metabolites, target gene transcripts and function analyses to reveal a gene-to-terpene network in tea plants provided the first reliable gene- to-terPene network for novel genes discovery.
Journal ArticleDOI
Composition analysis of floral scent within genus Camellia uncovers substantial interspecific variations
TL;DR: This work compared the volatile emission patterns between different floral organ types and developmental stages in Camellia buxifolia and revealed that stamens emitted more volatiles than petals, and the emission reached the maximum at the stage of flower half opening.
Journal ArticleDOI
Insights into the Intraspecific Variability of the above and Belowground Emissions of Volatile Organic Compounds in Tomato.
TL;DR: In this article, the in-vivo monitoring of volatile organic compound (VOC) emissions is a potential non-invasive tool in plant protection, especially in greenhouse cultivation, and the results underline that genetic variability, light-dependent de-novo synthesis, and belowground sources are factors to be considered for successful use in crop monitoring.
Book ChapterDOI
In planta transient expression analysis of monoterpene synthases.
TL;DR: This work describes a simple and cost-effective method for the in planta functional analysis of plant mono-TPS enzymes that can accommodate both the analysis of single genes and the scaling for more high-throughput functional screening of Mono-TPS gene families or mutant libraries.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
TL;DR: Version 4 of MEGA software expands on the existing facilities for editing DNA sequence data from autosequencers, mining Web-databases, performing automatic and manual sequence alignment, analyzing sequence alignments to estimate evolutionary distances, inferring phylogenetic trees, and testing evolutionary hypotheses.
Journal ArticleDOI
Multiple sequence alignment with the Clustal series of programs
Ramu Chenna,Hideaki Sugawara,Tadashi Koike,Rodrigo Lopez,Toby J. Gibson,Desmond G. Higgins,Julie D. Thompson +6 more
TL;DR: The Clustal series of programs, widely used in molecular biology for the multiple alignment of both nucleic acid and protein sequences and for preparing phylogenetic trees, are extended.
Journal ArticleDOI
The genome of black cottonwood, Populus trichocarpa (Torr. & Gray)
Gerald A. Tuskan,Gerald A. Tuskan,Stephen P. DiFazio,Stephen P. DiFazio,Stefan Jansson,Joerg Bohlmann,Igor V. Grigoriev,Uffe Hellsten,Nicholas H. Putnam,Steven G. Ralph,Stephane Rombauts,Asaf Salamov,Jacquie Schein,Lieven Sterck,Andrea Aerts,Rishikeshi Bhalerao,Rishikesh P. Bhalerao,Damien Blaudez,Wout Boerjan,Annick Brun,Amy M. Brunner,Victor Busov,Malcolm M. Campbell,John E. Carlson,Michel Chalot,Jarrod Chapman,G.-L. Chen,Dawn Cooper,Pedro M. Coutinho,Jérémy Couturier,Sarah F. Covert,Quentin C. B. Cronk,R. Cunningham,John M. Davis,Sven Degroeve,Annabelle Déjardin,Claude W. dePamphilis,John C. Detter,Bill Dirks,Inna Dubchak,Inna Dubchak,Sébastien Duplessis,Jürgen Ehlting,Brian E. Ellis,Karla C Gendler,David Goodstein,Michael Gribskov,Jane Grimwood,Andrew Groover,Lee E. Gunter,Björn Hamberger,Berthold Heinze,Yrjö Helariutta,Yrjö Helariutta,Yrjö Helariutta,Bernard Henrissat,D. Holligan,Robert A. Holt,Wenyu Huang,N. Islam-Faridi,Steven J.M. Jones,M. Jones-Rhoades,Richard A. Jorgensen,Chandrashekhar P. Joshi,Jaakko Kangasjärvi,Jan Karlsson,Colin T. Kelleher,Robert Kirkpatrick,Matias Kirst,Annegret Kohler,Udaya C. Kalluri,Frank W. Larimer,Jim Leebens-Mack,Jean-Charles Leplé,Philip F. LoCascio,Y. Lou,Susan Lucas,Francis Martin,Barbara Montanini,Carolyn A. Napoli,David R. Nelson,C D Nelson,Kaisa Nieminen,Ove Nilsson,V. Pereda,Gary F. Peter,Ryan N. Philippe,Gilles Pilate,Alexander Poliakov,J. Razumovskaya,Paul G. Richardson,Cécile Rinaldi,Kermit Ritland,Pierre Rouzé,D. Ryaboy,Jeremy Schmutz,J. Schrader,Bo Segerman,H. Shin,Asim Siddiqui,Fredrik Sterky,Astrid Terry,Chung-Jui Tsai,Edward C. Uberbacher,Per Unneberg,Jorma Vahala,Kerr Wall,Susan R. Wessler,Guojun Yang,T. Yin,Carl J. Douglas,Marco A. Marra,Göran Sandberg,Y. Van de Peer,Daniel S. Rokhsar,Daniel S. Rokhsar +115 more
TL;DR: The draft genome of the black cottonwood tree, Populus trichocarpa, has been reported in this paper, with more than 45,000 putative protein-coding genes identified.
Journal ArticleDOI
The map-based sequence of the rice genome
Takashi Matsumoto,Jianzhong Wu,Hiroyuki Kanamori,Yuichi Katayose,Masaki Fujisawa,Nobukazu Namiki,Hiroshi Mizuno,Kimiko Yamamoto,Baltazar A. Antonio,Tomoya Baba,Katsumi Sakata,Yoshiaki Nagamura,Hiroyoshi Aoki,Koji Arikawa,Kohei Arita,Takahito Bito,Yoshino Chiden,Nahoko Fujitsuka,Rie Fukunaka,Masao Hamada,Chizuko Harada,Akiko Hayashi,Saori Hijishita,Mikiko Honda,Satomi Hosokawa,Yoko Ichikawa,Atsuko Idonuma,Masumi Iijima,Michiko Ikeda,Maiko Ikeno,Kazue Ito,Sachie Ito,Tomoko Ito,Yuichi Ito,Yukiyo Ito,Aki Iwabuchi,Kozue Kamiya,Wataru Karasawa,Kanako Kurita,Satoshi Katagiri,Ari Kikuta,Harumi Kobayashi,Noriko Kobayashi,Kayo Machita,Tomoko Maehara,Masatoshi Masukawa,Tatsumi Mizubayashi,Yoshiyuki Mukai,Hideki Nagasaki,Yuko Nagata,Shinji Naito,Marina Nakashima,Yuko Nakama,Yumi Nakamichi,Mari Nakamura,Ayano Meguro,Manami Negishi,Isamu Ohta,Tomoya Ohta,Masako Okamoto,Nozomi Ono,Shoko Saji,Miyuki Sakaguchi,Kumiko Sakai,Michie Shibata,Takanori Shimokawa,Jianyu Song,Yuka Takazaki,Kimihiro Terasawa,Mika Tsugane,Kumiko Tsuji,Shigenori Ueda,Kazunori Waki,Harumi Yamagata,Mayu Yamamoto,Shinichi Yamamoto,Hiroko Yamane,Shoji Yoshiki,Rie Yoshihara,Kazuko Yukawa,Huisun Zhong,Masahiro Yano,Takuji Sasaki,Qiaoping Yuan,Shu Ouyang,Jia Liu,Kristine Jones,Kristen Gansberger,Kelly Moffat,Jessica Hill,Jayati Bera,Douglas Fadrosh,Shaohua Jin,Shivani Johri,Mary Kim,Larry Overton,Matthew Reardon,Tamara Tsitrin,Hue Vuong,Bruce Weaver,Anne Ciecko,Luke J. Tallon,Jacqueline Jackson,Grace Pai,Susan Van Aken,T. Utterback,Steve Reidmuller,Tamara Feldblyum,Joseph Hsiao,Victoria Zismann,Stacey E. Iobst,Aymeric R. De Vazeille,C. Robin Buell,Kai Ying,Ying Li,Tingting Lu,Y. Huang,Qiang Zhao,Qi Feng,Lei Zhang,Jingjie Zhu,Qijun Weng,Jie Mu,Yiqi Lu,Danlin Fan,Yilei Liu,Jianping Guan,Yujun Zhang,Shuliang Yu,Xiaohui Liu,Yu Zhang,Guofan Hong,Bin Han,Nathalie Choisne,Nadia Demange,Gisela Orjeda,Sylvie Samain,Laurence Cattolico,Eric Pelletier,Arnaud Couloux,Béatrice Segurens,Patrick Wincker,Angélique D'Hont,Claude Scarpelli,Jean Weissenbach,Marcel Salanoubat,Francis Quetier,Yeisoo Yu,Hyeran Kim,Teri Rambo,Jennifer Currie,Kristi Collura,Meizhong Luo,Tae-Jin Yang,Jetty S.S. Ammiraju,Friedrich Engler,Carol Soderlund,Rod A. Wing,Lance E. Palmer,Melissa De La Bastide,Lori Spiegel,Lidia Nascimento,Theresa Zutavern,A. O'Shaughnessy,Sujit Dike,Neilay Dedhia,R. Preston,Vivekanand Balija,W. Richard McCombie,Teh Yuan Chow,Hong-Hwa Chen,Mei-Chu Chung,Ching San Chen,J. F. Shaw,Hong Pang Wu,Kwang-Jen Hsiao,Ya Ting Chao,Mu Kuei Chu,Chia Hsiung Cheng,Ai Ling Hour,Pei Fang Lee,Shu Jen Lin,Yao-Cheng Lin,John Yu Liou,Shu Mei Liu,Yue-Ie C. Hsing,Saurabh Raghuvanshi,Amitabh Mohanty,Arvind K. Bharti,Arvind K. Bharti,Anupama Gaur,Vipin Gupta,Dibyendu Kumar,Vydianathan Ravi,Shubha Vij,Anita Kapur,Parul Khurana,Paramjit Khurana,Jitendra P. Khurana,Akhilesh K. Tyagi,Kishor Gaikwad,Alok Singh,Vivek Dalal,Subodh K. Srivastava,Anupam Dixit,Ajit K. Pal,Irfan Ahmad Ghazi,Mahavir Yadav,A. Pandit,Atul Bhargava,K. Sureshbabu,Kamlesh Batra,Tilak Raj Sharma,Trilochan Mohapatra,Nagendra K. Singh,Joachim Messing,Amy Bronzino Nelson,Galina Fuks,Steve Kavchok,Gladys Keizer,Eric Linton Victor Llaca,Rentao Song,Bahattin Tanyolac,Steve Young,Kim Yul Ho,Jang Ho Hahn,G. Sangsakoo,Apichart Vanavichit,Luiz Anderson Teixeira de Mattos,Paulo Dejalma Zimmer,Gaspar Malone,Odir Antônio Dellagostin,Antonio Costa de Oliveira,Michael W. Bevan,Ian Bancroft,Patrick Minx,Holly Cordum,Richard K. Wilson,Zhukuan Cheng,Weiwei Jin,Jiming Jiang,Sally A. Leong,Hisakazu Iwama,Takashi Gojobori,Takeshi Itoh,Takeshi Itoh,Yoshihito Niimura,Yasuyuki Fujii,Takuya Habara,Hiroaki Sakai,Hiroaki Sakai,Yoshiharu Sato,Gregory Wilson,Kiran Kumar,Susan R. McCouch,Nikoleta Juretic,Douglas R. Hoen,Stephen I. Wright,Richard Bruskiewich,Thomas E. Bureau,Akio Miyao,Hirohiko Hirochika,Tomotaro Nishikawa,Koh Ichi Kadowaki,Masahiro Sugiura,Benjamin Burr +265 more
TL;DR: A map-based, finished quality sequence that covers 95% of the 389 Mb rice genome, including virtually all of the euchromatin and two complete centromeres, and finds evidence for widespread and recurrent gene transfer from the organelles to the nuclear chromosomes.