The Tomato Terpene Synthase Gene Family
Vasiliki Falara,Tariq A. Akhtar,Thuong T.H. Nguyen,Eleni A. Spyropoulou,Petra M. Bleeker,Ines Schauvinhold,Yuki Matsuba,Megan E. Bonini,Anthony L. Schilmiller,Robert C. Schuurink,Eran Pichersky +10 more
TLDR
In this paper, it was shown that the tomato genome contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional in at least some organs or tissues of the plant.Abstract:
Compounds of the terpenoid class play numerous roles in the interactions of plants with their environment, such as attracting pollinators and defending the plant against pests. We show here that the genome of cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional. Of these 29 TPS genes, 26 were expressed in at least some organs or tissues of the plant. The enzymatic functions of eight of the TPS proteins were previously reported, and here we report the specific in vitro catalytic activity of 10 additional tomato terpene synthases. Many of the tomato TPS genes are found in clusters, notably on chromosomes 1, 2, 6, 8, and 10. All TPS family clades previously identified in angiosperms are also present in tomato. The largest clade of functional TPS genes found in tomato, with 12 members, is the TPS-a clade, and it appears to encode only sesquiterpene synthases, one of which is localized to the mitochondria, while the rest are likely cytosolic. A few additional sesquiterpene synthases are encoded by TPS-b clade genes. Some of the tomato sesquiterpene synthases use z,z-farnesyl diphosphate in vitro as well, or more efficiently than, the e,e-farnesyl diphosphate substrate. Genes encoding monoterpene synthases are also prevalent, and they fall into three clades: TPS-b, TPS-g, and TPS-e/f. With the exception of two enzymes involved in the synthesis of ent-kaurene, the precursor of gibberellins, no other tomato TPS genes could be demonstrated to encode diterpene synthases so far.read more
Citations
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Genome-Wide Identification and Expression Profile of TPS Gene Family in Dendrobium officinale and the Role of DoTPS10 in Linalool Biosynthesis.
TL;DR: The results simultaneously provide valuable insight into understanding the roles of the TPS family and lay a basis for further studies on the regulation of terpenoid biosynthesis by DoTPS by integrating the D. officinale genome and transcriptional data.
Journal ArticleDOI
Secretions from the ventral eversible gland of Spodoptera exigua caterpillars activate defense-related genes and induce emission of volatile organic compounds in tomato, Solanum lycopersicum
TL;DR: The results suggest that the VEG of S. exigua caterpillars contains elicitors of late plant defense signaling in tomato which trigger defense-related enzymatic activity, regulate expression ofdefense-related genes, and induce emission of plant VOCs.
Journal ArticleDOI
Genomic Clues for Crop-Weed Interactions and Evolution.
TL;DR: Recent genome-based research studies on agricultural weeds and crop-weed interactions that reveal several major evolutionary innovations such as de-domestication, interactions mediated by allelochemical secondary metabolites, and parasitic genetic elements that play crucial roles in enhancing weed invasiveness in agricultural settings are discussed.
Journal ArticleDOI
Capturing flavors from Capsicum baccatum by introgression in sweet pepper.
P.M. Eggink,Yury Tikunov,Chris Maliepaard,J.P.W. Haanstra,H. de Rooij,Aat Vogelaar,E.W. Gutteling,G. Freymark,Arnaud G. Bovy,Richard G. F. Visser +9 more
TL;DR: Biochemical characterization in combination with genetic analyses in BC2S1plants and near-isogenic lines led to the detection and validation of C. baccatumloci affecting flavor, terpenoid content and Brix level, resulting in confirmed genetic effects for physical, biochemical and sensory traits.
Journal ArticleDOI
Metabolic engineering of monoterpene biosynthesis in tomato fruits via introduction of the non-canonical substrate neryl diphosphate
Michael Gutensohn,Thuong T.H. Nguyen,Richard D. McMahon,Ian Kaplan,Eran Pichersky,Natalia Dudareva +5 more
TL;DR: The results show that NPP-derived terpenoids can be produced in plant tissues; however, NPP has to be consumed to avoid negative impacts on plant metabolism.
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TL;DR: The draft genome of the black cottonwood tree, Populus trichocarpa, has been reported in this paper, with more than 45,000 putative protein-coding genes identified.
Journal ArticleDOI
The map-based sequence of the rice genome
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TL;DR: A map-based, finished quality sequence that covers 95% of the 389 Mb rice genome, including virtually all of the euchromatin and two complete centromeres, and finds evidence for widespread and recurrent gene transfer from the organelles to the nuclear chromosomes.