The Tomato Terpene Synthase Gene Family
Vasiliki Falara,Tariq A. Akhtar,Thuong T.H. Nguyen,Eleni A. Spyropoulou,Petra M. Bleeker,Ines Schauvinhold,Yuki Matsuba,Megan E. Bonini,Anthony L. Schilmiller,Robert C. Schuurink,Eran Pichersky +10 more
TLDR
In this paper, it was shown that the tomato genome contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional in at least some organs or tissues of the plant.Abstract:
Compounds of the terpenoid class play numerous roles in the interactions of plants with their environment, such as attracting pollinators and defending the plant against pests. We show here that the genome of cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional. Of these 29 TPS genes, 26 were expressed in at least some organs or tissues of the plant. The enzymatic functions of eight of the TPS proteins were previously reported, and here we report the specific in vitro catalytic activity of 10 additional tomato terpene synthases. Many of the tomato TPS genes are found in clusters, notably on chromosomes 1, 2, 6, 8, and 10. All TPS family clades previously identified in angiosperms are also present in tomato. The largest clade of functional TPS genes found in tomato, with 12 members, is the TPS-a clade, and it appears to encode only sesquiterpene synthases, one of which is localized to the mitochondria, while the rest are likely cytosolic. A few additional sesquiterpene synthases are encoded by TPS-b clade genes. Some of the tomato sesquiterpene synthases use z,z-farnesyl diphosphate in vitro as well, or more efficiently than, the e,e-farnesyl diphosphate substrate. Genes encoding monoterpene synthases are also prevalent, and they fall into three clades: TPS-b, TPS-g, and TPS-e/f. With the exception of two enzymes involved in the synthesis of ent-kaurene, the precursor of gibberellins, no other tomato TPS genes could be demonstrated to encode diterpene synthases so far.read more
Citations
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Identification and Characterization of Terpene Synthases Potentially Involved in the Formation of Volatile Terpenes in Carrot (Daucus carota L.) Roots
TL;DR: A transcriptome-based identification and functional characterization of two carrot terpene synthases suggest a role for DcTPS genes in carrot aroma biosynthesis.
Journal ArticleDOI
Analysis of the expression of terpene synthase genes in relation to aroma content in two aromatic Vitis vinifera varieties
TL;DR: The genes identified in this study as being mainly responsible for linalool and geraniol synthesis during berry development, and the phenological phases in which they are mostly expressed, should be of interest to viticulturists and wine makers to improve decision making along the chain of production.
Journal ArticleDOI
The Terpene Synthase Gene Family of Carrot (Daucus carota L.): Identification of QTLs and Candidate Genes Associated with Terpenoid Volatile Compounds.
Jens Keilwagen,Heike Lehnert,Thomas Berner,Holger Budahn,Thomas Nothnagel,Detlef Ulrich,Frank Dunemann +6 more
TL;DR: A combination of terpene metabolite profiling, genotyping-by-sequencing (GBS), and genome-wide association study (GWAS) was used to get insights into the genetic control of terPene biosynthesis in carrots and to identify several TPS candidate genes that might be involved in the production of specific monoterpenes.
Journal ArticleDOI
Analysis of Terpene Synthase Family Genes in Camellia sinensis with an Emphasis on Abiotic Stress Conditions.
TL;DR: In this article, global terpenoid synthase gene (TPS) transcription analysis was conducted based on transcriptomic data combined with terpenoids metabolic profiling under different abiotic stress conditions.
Journal ArticleDOI
Telomere-to-telomere gapless chromosomes of banana using nanopore sequencing.
Caroline Belser,Franc-Christophe Baurens,Benjamin Noel,Guillaume Martin,Corinne Cruaud,Benjamin Istace,Nabila Yahiaoui,Karine Labadie,Eva Hřibová,Jaroslav Doležel,Arnaud Lemainque,Patrick Wincker,Angélique D'Hont,Jean-Marc Aury +13 more
TL;DR: In this paper, the authors reported a chromosome-scale assembly of a banana genome (Musa acuminata) generated using Oxford Nanopore long-reads and achieved genome coverage of 177X from a single PromethION flowcell with near 17X with reads longer than 75 kbp.
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TL;DR: The draft genome of the black cottonwood tree, Populus trichocarpa, has been reported in this paper, with more than 45,000 putative protein-coding genes identified.
Journal ArticleDOI
The map-based sequence of the rice genome
Takashi Matsumoto,Jianzhong Wu,Hiroyuki Kanamori,Yuichi Katayose,Masaki Fujisawa,Nobukazu Namiki,Hiroshi Mizuno,Kimiko Yamamoto,Baltazar A. Antonio,Tomoya Baba,Katsumi Sakata,Yoshiaki Nagamura,Hiroyoshi Aoki,Koji Arikawa,Kohei Arita,Takahito Bito,Yoshino Chiden,Nahoko Fujitsuka,Rie Fukunaka,Masao Hamada,Chizuko Harada,Akiko Hayashi,Saori Hijishita,Mikiko Honda,Satomi Hosokawa,Yoko Ichikawa,Atsuko Idonuma,Masumi Iijima,Michiko Ikeda,Maiko Ikeno,Kazue Ito,Sachie Ito,Tomoko Ito,Yuichi Ito,Yukiyo Ito,Aki Iwabuchi,Kozue Kamiya,Wataru Karasawa,Kanako Kurita,Satoshi Katagiri,Ari Kikuta,Harumi Kobayashi,Noriko Kobayashi,Kayo Machita,Tomoko Maehara,Masatoshi Masukawa,Tatsumi Mizubayashi,Yoshiyuki Mukai,Hideki Nagasaki,Yuko Nagata,Shinji Naito,Marina Nakashima,Yuko Nakama,Yumi Nakamichi,Mari Nakamura,Ayano Meguro,Manami Negishi,Isamu Ohta,Tomoya Ohta,Masako Okamoto,Nozomi Ono,Shoko Saji,Miyuki Sakaguchi,Kumiko Sakai,Michie Shibata,Takanori Shimokawa,Jianyu Song,Yuka Takazaki,Kimihiro Terasawa,Mika Tsugane,Kumiko Tsuji,Shigenori Ueda,Kazunori Waki,Harumi Yamagata,Mayu Yamamoto,Shinichi Yamamoto,Hiroko Yamane,Shoji Yoshiki,Rie Yoshihara,Kazuko Yukawa,Huisun Zhong,Masahiro Yano,Takuji Sasaki,Qiaoping Yuan,Shu Ouyang,Jia Liu,Kristine Jones,Kristen Gansberger,Kelly Moffat,Jessica Hill,Jayati Bera,Douglas Fadrosh,Shaohua Jin,Shivani Johri,Mary Kim,Larry Overton,Matthew Reardon,Tamara Tsitrin,Hue Vuong,Bruce Weaver,Anne Ciecko,Luke J. Tallon,Jacqueline Jackson,Grace Pai,Susan Van Aken,T. Utterback,Steve Reidmuller,Tamara Feldblyum,Joseph Hsiao,Victoria Zismann,Stacey E. Iobst,Aymeric R. De Vazeille,C. Robin Buell,Kai Ying,Ying Li,Tingting Lu,Y. Huang,Qiang Zhao,Qi Feng,Lei Zhang,Jingjie Zhu,Qijun Weng,Jie Mu,Yiqi Lu,Danlin Fan,Yilei Liu,Jianping Guan,Yujun Zhang,Shuliang Yu,Xiaohui Liu,Yu Zhang,Guofan Hong,Bin Han,Nathalie Choisne,Nadia Demange,Gisela Orjeda,Sylvie Samain,Laurence Cattolico,Eric Pelletier,Arnaud Couloux,Béatrice Segurens,Patrick Wincker,Angélique D'Hont,Claude Scarpelli,Jean Weissenbach,Marcel Salanoubat,Francis Quetier,Yeisoo Yu,Hyeran Kim,Teri Rambo,Jennifer Currie,Kristi Collura,Meizhong Luo,Tae-Jin Yang,Jetty S.S. Ammiraju,Friedrich Engler,Carol Soderlund,Rod A. Wing,Lance E. Palmer,Melissa De La Bastide,Lori Spiegel,Lidia Nascimento,Theresa Zutavern,A. O'Shaughnessy,Sujit Dike,Neilay Dedhia,R. Preston,Vivekanand Balija,W. Richard McCombie,Teh Yuan Chow,Hong-Hwa Chen,Mei-Chu Chung,Ching San Chen,J. F. Shaw,Hong Pang Wu,Kwang-Jen Hsiao,Ya Ting Chao,Mu Kuei Chu,Chia Hsiung Cheng,Ai Ling Hour,Pei Fang Lee,Shu Jen Lin,Yao-Cheng Lin,John Yu Liou,Shu Mei Liu,Yue-Ie C. Hsing,Saurabh Raghuvanshi,Amitabh Mohanty,Arvind K. Bharti,Arvind K. Bharti,Anupama Gaur,Vipin Gupta,Dibyendu Kumar,Vydianathan Ravi,Shubha Vij,Anita Kapur,Parul Khurana,Paramjit Khurana,Jitendra P. Khurana,Akhilesh K. Tyagi,Kishor Gaikwad,Alok Singh,Vivek Dalal,Subodh K. Srivastava,Anupam Dixit,Ajit K. Pal,Irfan Ahmad Ghazi,Mahavir Yadav,A. Pandit,Atul Bhargava,K. Sureshbabu,Kamlesh Batra,Tilak Raj Sharma,Trilochan Mohapatra,Nagendra K. Singh,Joachim Messing,Amy Bronzino Nelson,Galina Fuks,Steve Kavchok,Gladys Keizer,Eric Linton Victor Llaca,Rentao Song,Bahattin Tanyolac,Steve Young,Kim Yul Ho,Jang Ho Hahn,G. Sangsakoo,Apichart Vanavichit,Luiz Anderson Teixeira de Mattos,Paulo Dejalma Zimmer,Gaspar Malone,Odir Antônio Dellagostin,Antonio Costa de Oliveira,Michael W. Bevan,Ian Bancroft,Patrick Minx,Holly Cordum,Richard K. Wilson,Zhukuan Cheng,Weiwei Jin,Jiming Jiang,Sally A. Leong,Hisakazu Iwama,Takashi Gojobori,Takeshi Itoh,Takeshi Itoh,Yoshihito Niimura,Yasuyuki Fujii,Takuya Habara,Hiroaki Sakai,Hiroaki Sakai,Yoshiharu Sato,Gregory Wilson,Kiran Kumar,Susan R. McCouch,Nikoleta Juretic,Douglas R. Hoen,Stephen I. Wright,Richard Bruskiewich,Thomas E. Bureau,Akio Miyao,Hirohiko Hirochika,Tomotaro Nishikawa,Koh Ichi Kadowaki,Masahiro Sugiura,Benjamin Burr +265 more
TL;DR: A map-based, finished quality sequence that covers 95% of the 389 Mb rice genome, including virtually all of the euchromatin and two complete centromeres, and finds evidence for widespread and recurrent gene transfer from the organelles to the nuclear chromosomes.