Variable numbers of pepsinogen genes are located in the centromeric region of human chromosome 11 and determine the high-frequency electrophoretic polymorphism.
TLDR
It is demonstrated that this genetic polymorphism involving intensity variation of individual pepsinogen isozymogens results from chromosome haplotypes that contain different numbers of genes.Abstract:
A panel of 26 mouse-human somatic cell hybrids containing different human chromosome complements was analyzed with a cloned human pepsinogen cDNA probe to determine the chromosomal location and the number of genes encoding these proteins. A complex containing variable numbers of pepsinogen genes was localized to the centromeric region of human chromosome 11 (p11----q13). Examination of somatic cell hybrids containing single copies of chromosome 11 and the corresponding human parental cell lines revealed a restriction fragment length polymorphism determined by pepsinogen haplotypes that contained two or three genes, respectively. Concurrent studies of DNA from individuals exhibiting the most common pepsinogen electrophoretic phenotypes with exon-specific probes demonstrated that the absence of one gene among the different restriction fragment patterns correlated with the absence of one specific isozymogen (Pg 5). Thus, our studies demonstrate that this genetic polymorphism involving intensity variation of individual pepsinogen isozymogens results from chromosome haplotypes that contain different numbers of genes. The regional localization of this polymorphic gene complex will facilitate detailed linkage analysis of human chromosome 11.read more
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References
More filters
Journal ArticleDOI
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing
TL;DR: An approach to DNA sequencing using chain-terminating inhibitors (Sanger et al., 1977) combined with cloning of small fragments of DNA in a single-stranded DNA bacteriophage is described, determining the 2771-nucleotide sequence of the largest MboI restriction enzyme fragment from human mitochondrial DNA.
Journal ArticleDOI
Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing
Michael G. Rosenfeld,Jean-Jacques Mermod,Susan G. Amara,Larry W. Swanson,Paul E. Sawchenko,Jean Rivier,Wylie Vale,Ronald M. Evans +7 more
TL;DR: The approach described here permits the application of recombinant DNA technology to analyses of complex neurobiological systems in the absence of prior structural or biological information.
Journal ArticleDOI
Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products
TL;DR: A model in which developmental regulation of RNA processing is used to increase the diversity of neuroendocrine gene expression is proposed.
Journal ArticleDOI
A Method for Isolation of Intact, Translationally Active Ribonucleic Acid
Guy Cathala,Jean-François Savouret,Bernardita Mendez,Brian L. West,Michael Karin,Joseph Martial,John D. Baxter +6 more
TL;DR: A method for isolation of large, translationally active RNA species is presented and yields large mRNA precursors up to 10 kb and mRNA species which translate very well, however, small RNA species are recovered with a poor yield.
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Polymorphic DNA region adjacent to the 5' end of the human insulin gene
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