D
David T. Breault
Researcher at Boston Children's Hospital
Publications - 117
Citations - 5335
David T. Breault is an academic researcher from Boston Children's Hospital. The author has contributed to research in topics: Stem cell & Biology. The author has an hindex of 30, co-authored 92 publications receiving 4040 citations. Previous affiliations of David T. Breault include University of Connecticut Health Center & Harvard University.
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Journal ArticleDOI
Defining Molecular Cornerstones during Fibroblast to iPS Cell Reprogramming in Mouse
TL;DR: Using doxycycline-inducible vectors, it is shown that exogenous factors are required for about 10 days, after which cells enter a self-sustaining pluripotent state and markers are identified that define cell populations prior to and during this transition period.
Journal ArticleDOI
Mouse telomerase reverse transcriptase (mTert) expression marks slowly cycling intestinal stem cells
Robert K. Montgomery,Diana L. Carlone,Camilla A. Richmond,Loredana G. Farilla,Mariette E. G. Kranendonk,Daniel E. Henderson,Nana Yaa Baffour-Awuah,Dana M. Ambruzs,Laura K. Fogli,Selma Algra,David T. Breault +10 more
TL;DR: It is demonstrated that a slowly cycling stem cell population exists within the intestine and that mTert+ cells give rise to all differentiated intestinal cell types, persist long term, and contribute to the regenerative response following injury.
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Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids
Magdalena Kasendra,Alessio Tovaglieri,Alessio Tovaglieri,Alexandra Sontheimer-Phelps,Alexandra Sontheimer-Phelps,Sasan Jalili-Firoozinezhad,Sasan Jalili-Firoozinezhad,Amir Bein,Angeliki Chalkiadaki,William Scholl,Cheng Zhang,Hannah Rickner,Camilla A. Richmond,Hu Li,David T. Breault,David T. Breault,Donald E. Ingber,Donald E. Ingber,Donald E. Ingber +18 more
TL;DR: The Intestine Chip may be useful as a research tool for applications where normal intestinal function is crucial, including studies of metabolism, nutrition, infection, and drug pharmacokinetics, as well as personalized medicine.
Journal ArticleDOI
A Complex Human-Gut Microbiome Cultured in an Anaerobic Intestine-on-a-Chip
Sasan Jalili-Firoozinezhad,Sasan Jalili-Firoozinezhad,Francesca S. Gazzaniga,Francesca S. Gazzaniga,Elizabeth Calamari,Diogo M. Camacho,Cicely W. Fadel,Amir Bein,Ben Swenor,Bret Nestor,Michael J. Cronce,Alessio Tovaglieri,Alessio Tovaglieri,Oren Levy,Katherine E. Gregory,Katherine E. Gregory,David T. Breault,David T. Breault,Joaquim M. S. Cabral,Dennis L. Kasper,Richard M. Novak,Donald E. Ingber,Donald E. Ingber,Donald E. Ingber +23 more
TL;DR: The extended coculture of living human intestinal epithelium with stable communities of aerobic and anaerobic human gut microbiota is shown, using a microfluidic intestine-on-a-chip that permits the control and real-time assessment of physiologically relevant oxygen gradients.
Journal ArticleDOI
Intestinal enteroendocrine lineage cells possess homeostatic and injury-inducible stem cell activity
Kelley S. Yan,Kelley S. Yan,Olivier Gevaert,Grace X.Y. Zheng,Benedict Anchang,Chris Probert,Kathryn A. Larkin,Paige S. Davies,Zhuan fen Cheng,John S. Kaddis,Arnold Han,Arnold Han,Kelly Roelf,Ruben I. Calderon,Esther Cynn,Xiaoyi Hu,Komal Mandleywala,Julie Wilhelmy,Susan M. Grimes,David C Corney,Stéphane C. Boutet,Jessica M. Terry,Phillip Belgrader,Solongo B. Ziraldo,Tarjei S. Mikkelsen,Fengchao Wang,Richard J. von Furstenberg,Nicholas R. Smith,Parthasarathy Chandrakesan,Randal May,Mary Ann S. Chrissy,Rajan Jain,Christine A. Cartwright,Joyce C. Niland,Young-Kwon Hong,Jill L. Carrington,David T. Breault,Jonathan I. Epstein,Courtney W. Houchen,John P. Lynch,Martin G. Martin,Sylvia K. Plevritis,Christina Curtis,Hanlee P. Ji,Linheng Li,Susan J. Henning,Melissa H. Wong,Calvin J. Kuo +47 more
TL;DR: The data suggest that the EE lineage, including mature EE cells, comprises a reservoir of homeostatic and injury-inducible ISCs, extending the understanding of cellular plasticity and stemness.