Ulrike Boehm

TL;DR: The QUAREP-LiMi project as mentioned in this paper aims to improve reproducibility for light microscopy image data through quality control (QC) management of instruments and images through a common set of QC guidelines for hardware calibration and image acquisition, management and analysis.

TL;DR: The QUAREP-LiMi initiative as mentioned in this paper aims to improve the overall quality and reproducibility of light microscope image data by introducing broadly accepted standard practices and accurately captured image data metrics.

TL;DR: The 4D Nucleome Initiative (4DN) and the BioImaging North America (BINA)-OME (NBO namespace) as mentioned in this paper have proposed a set of metadata specifications for light microscopy data that scale with experimental intent and with the complexity of the instrumentation and analytical requirements.

TL;DR: The 4D Nucleome Initiative (4DN) and the BioImaging North America (BINA)-OME (NBO namespace) as mentioned in this paper have proposed a set of metadata specifications for light microscopy data that scale with experimental intent and with the complexity of the instrumentation and analytical requirements.

Abstract: In April 2020, the QUality Assessment and REProducibility for Instruments and Images in Light Microscopy (QUAREP-LiMi) initiative was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality control (QC) in light microscopy. The ultimate goal of the QUAREP-LiMi initiative is to establish a set of common QC standards, guidelines, metadata models, and tools, including detailed protocols, with the ultimate aim of improving reproducible advances in scientific research. This White Paper 1) summarizes the major obstacles identified in the field that motivated the launch of the QUAREP-LiMi initiative; 2) identifies the urgent need to address these obstacles in a grassroots manner, through a community of stakeholders including, researchers, imaging scientists, bioimage analysts, bioimage informatics developers, corporate partners, funding agencies, standards organizations, scientific publishers, and observers of such; 3) outlines the current actions of the QUAREP-LiMi initiative, and 4) proposes future steps that can be taken to improve the dissemination and acceptance of the proposed guidelines to manage QC. To summarize, the principal goal of the QUAREP-LiMi initiative is to improve the overall quality and reproducibility of light microscope image data by introducing broadly accepted standard practices and accurately captured image data metrics.