Government of India

About: Government of India is a government organization based out in New Delhi, India. It is known for research contribution in the topics: Population & Government. The organization has 2945 authors who have published 2999 publications receiving 44942 citations. The organization is also known as: Union Government & Central Government.

The Starch of Pueraria tuberosa — Comparison with Maize Starch

Abstract: Pueraria tuberosa starch was isolated from the tuber and purified. Scanning electron microscopy of the starch showed polygonal shaped granules which were almost of the same particle size as those of maize starch. P. tuberosa starch has lower amylose content, almost same gelatinization temperature range and water binding capacity, more swelling and solubility compared to maize starch. Paste viscosity characteristics show high peak viscosity but also indicate fragile nature of granules in comparison to maize starch.

Microwave Dielectric Properties of Low‐Temperature Sinterable BaCe2(MoO4)4 Ceramics

Abstract: Low-temperature sinterable BaCe2(MoO4)4 ceramics were prepared through solid-state ceramic route. X-ray diffraction, laser Raman spectroscopy and scanning electron microscopy techniques have been used to investigate the structure and microstructure of BaCe2(MoO4)4 ceramics. The laser Raman study reveals distorted MoO42− tetrahedral networks in the unit cell of the BaCe2(MoO4)4 ceramics. The BaCe2(MoO4)4 exhibits a dielectric constant of 12.3, an unloaded quality factor of 3320, and a temperature coefficient of resonant frequency of −37 ppm/°C.

Comparative analysis of synthetic DNA promoters for high-level gene expression in plants

Abstract: Main conclusion We have designed two near-constitutive and stress-inducible promoters (CmYLCV9.11 and CmYLCV4); those are highly efficient in both dicot and monocot plants and have prospective to substitute the CaMV 35S promoter. We performed structural and functional studies of the full-length transcript promoter from Cestrum yellow leaf curling virus (CmYLCV) employing promoter/leader deletion and activating cis-sequence analysis. We designed a 465-bp long CmYLCV9.11 promoter fragment (−329 to +137 from transcription start site) that showed enhanced promoter activity and was highly responsive to both biotic and abiotic stresses. The CmYLCV9.11 promoter was about 28-fold stronger than the CaMV35S promoter in transient and stable transgenic assays using β-glucuronidase (GUS) reporter gene. The CmYLCV9.11 promoter also demonstrated stronger activity than the previously reported CmYLCV promoter fragments, CmpC (−341 to +5) and CmpS (−349 to +59) in transient systems like maize protoplasts and onion epidermal cells as well as transgenic systems. A good correlation between CmYLCV9.11 promoter-driven GUS-accumulation/enzymatic activities with corresponding uidA-mRNA level in transgenic tobacco plants was shown. Histochemical (X-Gluc) staining of transgenic seedlings, root and floral parts expressing the GUS under the control of CmYLCV9.11, CaMV35S, CmpC and CmpS promoters also support the above findings. The CmYLCV9.11 promoter is a constitutive promoter and the expression level in tissues of transgenic tobacco plants was in the following order: root > leaf > stem. The tobacco transcription factor TGA1a was found to bind strongly to the CmYLCV9.11 promoter region, as shown by Gel-shift assay and South-Western blot analysis. In addition, the CmYLCV9.11 promoter was regulated by a number of abiotic and biotic stresses as studied in transgenic Arabidopsis and tobacco plants. The newly derived CmYLCV9.11 promoter is an efficient tool for biotechnological applications.