Institution
ICFO – The Institute of Photonic Sciences
Facility•Barcelona, Spain•
About: ICFO – The Institute of Photonic Sciences is a facility organization based out in Barcelona, Spain. It is known for research contribution in the topics: Quantum & Quantum entanglement. The organization has 872 authors who have published 1965 publications receiving 56273 citations.
Topics: Quantum, Quantum entanglement, Plasmon, Graphene, Photon
Papers published on a yearly basis
Papers
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TL;DR: A first profile of the molecular events related to the dynamics of crocetin and crocin accumulation during tepal development is provided, and new information concerning apocarotenoid biosynthesis regulators and their accumulation in Crocus is presented.
Abstract: Crocins, the red soluble apocarotenoids of saffron, accumulate in the flowers of Crocus species in a developmental and tissue-specific manner In Crocus sieberi, crocins accumulate in stigmas but also in a distinct yellow tepal sector, which we demonstrate contains chromoplast converted from amyloplasts Secondary metabolites were analysed by LC-DAD-HRMS, revealing the progressive accumulation of crocetin and crocins in the yellow sector, which were also localized in situ by Raman microspectroscopy To understand the underlying mechanisms of crocin biosynthesis, we sequenced the C sieberi tepal transcriptome of two differentially pigmented sectors (yellow and white) at two developmental stages (6 and 8) by Illumina sequencing A total of 154 million high-quality reads were generated and assembled into 248,099 transcripts Differentially expressed gene analysis resulted in the identification of several potential candidate genes involved in crocin metabolism and regulation The results provide a first profile of the molecular events related to the dynamics of crocetin and crocin accumulation during tepal development, and present new information concerning apocarotenoid biosynthesis regulators and their accumulation in Crocus Further, reveals genes that were previously unknown to affect crocin formation, which could be used to improve crocin accumulation in Crocus plants and the commercial quality of saffron spice
37 citations
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University of Southern California1, Federal Fluminense University2, ICFO – The Institute of Photonic Sciences3, University of Melbourne4, Walter and Eliza Hall Institute of Medical Research5, Catalan Institution for Research and Advanced Studies6, University of Pennsylvania7, University of Barcelona8
TL;DR: This protocol describes enhanced number and brightness (eN&B), an approach that uses fluorescence fluctuation spectroscopy data to directly measure the oligomerization state and dynamics of fluorescently tagged proteins in living cells.
Abstract: Protein dimerization and oligomerization are essential to most cellular functions, yet measurement of the size of these oligomers in live cells, especially when their size changes over time and space, remains a challenge. A commonly used approach for studying protein aggregates in cells is number and brightness (N&B), a fluorescence microscopy method that is capable of measuring the apparent average number of molecules and their oligomerization (brightness) in each pixel from a series of fluorescence microscopy images. We have recently expanded this approach in order to allow resampling of the raw data to resolve the statistical weighting of coexisting species within each pixel. This feature makes enhanced N&B (eN&B) optimal for capturing the temporal aspects of protein oligomerization when a distribution of oligomers shifts toward a larger central size over time. In this protocol, we demonstrate the application of eN&B by quantifying receptor clustering dynamics using electron-multiplying charge-coupled device (EMCCD)-based total internal reflection microscopy (TIRF) imaging. TIRF provides a superior signal-to-noise ratio, but we also provide guidelines for implementing eN&B in confocal microscopes. For each time point, eN&B requires the acquisition of 200 frames, and it takes a few seconds up to 2 min to complete a single time point. We provide an eN&B (and standard N&B) MATLAB software package amenable to any standard confocal or TIRF microscope. The software requires a high-RAM computer (64 Gb) to run and includes a photobleaching detrending algorithm, which allows extension of the live imaging for more than an hour. This protocol describes enhanced number and brightness (eN&B), an approach that uses fluorescence fluctuation spectroscopy data to directly measure the oligomerization state and dynamics of fluorescently tagged proteins in living cells.
37 citations
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TL;DR: This review is an attempt to guide the reader into the possibilities that AO scanning laser ophthalmoscopy offers, as well as its limitations, which should be taken into account when planning on using it.
Abstract: Adaptive optics (AO) retinal imaging has become very popular in the past few years, especially within the ophthalmic research community. Several different retinal techniques, such as fundus imaging cameras or optical coherence tomography systems, have been coupled with AO in order to produce impressive images showing individual cell mosaics over different layers of the in vivo human retina. The combination of AO with scanning laser ophthalmoscopy has been extensively used to generate impressive images of the human retina with unprecedented resolution, showing individual photoreceptor cells, retinal pigment epithelium cells, as well as microscopic capillary vessels, or the nerve fiber layer. Over the past few years, the technique has evolved to develop several different applications not only in the clinic but also in different animal models, thanks to technological developments in the field. These developments have specific applications to different fields of investigation, which are not limited to the study of retinal diseases but also to the understanding of the retinal function and vision science. This review is an attempt to summarize these developments in an understandable and brief manner in order to guide the reader into the possibilities that AO scanning laser ophthalmoscopy offers, as well as its limitations, which should be taken into account when planning on using it.
37 citations
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TL;DR: Preliminary nonlinear imaging investigations indicate that this system is promising as a high peak-power pulsed light source for nonlinear bio-imaging applications across the 1.0 μm - 1.3 μm spectral range.
Abstract: In this paper, we present the generation of high peak-power picosecond optical pulses in the 1.26 μm spectral band from a repetition-rate-tunable quantum-dot external-cavity passively mode-locked laser (QD-ECMLL), amplified by a tapered quantum-dot semiconductor optical amplifier (QD-SOA). The laser emission wavelength was controlled through a chirped volume Bragg grating which was used as an external cavity output coupler. An average power of 208.2 mW, pulse energy of 321 pJ, and peak power of 30.3 W were achieved. Preliminary nonlinear imaging investigations indicate that this system is promising as a high peak-power pulsed light source for nonlinear bio-imaging applications across the 1.0 μm - 1.3 μm spectral range.
36 citations
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TL;DR: It is experimentally demonstrated that the interaction between a relativistic electron and vibrational modes in nanostructures is fundamentally modified in the presence of plasmons, holding great potential for investigating sensing mechanisms and chemistry in complex nanomaterials down to the molecular level.
Abstract: Atomic vibrations and phonons are an excellent source of information on nanomaterials that we can access through a variety of methods including Raman scattering, infrared spectroscopy, and electron energy-loss spectroscopy (EELS). In the presence of a plasmon local field, vibrations are strongly modified and, in particular, their dipolar strengths are highly enhanced, thus rendering Raman scattering and infrared spectroscopy extremely sensitive techniques. Here, we experimentally demonstrate that the interaction between a relativistic electron and vibrational modes in nanostructures is fundamentally modified in the presence of plasmons. We finely tune the energy of surface plasmons in metallic nanowires in the vicinity of hexagonal boron nitride, making it possible to monitor and disentangle both strong phonon-plasmon coupling and plasmon-driven phonon enhancement at the nanometer scale. Because of the near-field character of the electron beam-phonon interaction, optically inactive phonon modes are also observed. Besides increasing our understanding of phonon physics, our results hold great potential for investigating sensing mechanisms and chemistry in complex nanomaterials down to the molecular level.
36 citations
Authors
Showing all 928 results
Name | H-index | Papers | Citations |
---|---|---|---|
Maciej Lewenstein | 104 | 931 | 47362 |
F. Javier García de Abajo | 75 | 351 | 30221 |
Antonio Acín | 72 | 324 | 19984 |
Frank H. L. Koppens | 69 | 239 | 32754 |
Romain Quidant | 68 | 248 | 18262 |
Leszek Kaczmarek | 67 | 302 | 15985 |
Sefaattin Tongay | 65 | 254 | 20628 |
Zhipei Sun | 65 | 270 | 27030 |
Lluis Torner | 64 | 566 | 17978 |
Georg Heinze | 63 | 354 | 16391 |
Yaroslav V. Kartashov | 54 | 487 | 11174 |
Francesco Ricci | 54 | 295 | 15492 |
Gerasimos Konstantatos | 53 | 160 | 19627 |
Niek F. van Hulst | 53 | 178 | 12400 |
Turgut Durduran | 53 | 289 | 10525 |