Showing papers by "Michigan Technological University published in 2002"

Fine root architecture of nine north american trees

Abstract: The fine roots of trees are concentrated on lateral branches that arise from perennial roots. They are important in the acquisition of water and essential nutrients, and at the ecosystem level, they make a significant contribution to biogeochemical cycling. Fine roots have often been studied according to arbitrary size classes, e.g., all roots less than 1 or 2 mm in diameter. Because of the size class approach, the position of an individual root on the complex lateral branching system has often been ignored, and relationships between the form of the branching root system and its function are poorly understood. The fine roots of both gymnosperms and angiosperms, which formed ectomycorrhizae (EM) and arbuscular mycorrhizae (AM) fungal associations, were sampled in 1998 and 1999. Study sites were chosen to encompass a wide variety of environments in four regions of North America. Intact lateral branches were collected from each species and 18 561 individual roots were dissected by order, with distal roots numbered as first-order roots. This scheme is similar to the one commonly used to number the order of streams. Fine root diameter, length, specific root length (SRL; m/g), and nitrogen (N) concentration of nine North American tree species (Acer saccharunz, Juniperus monosperma, Liriodendron tulipifera, Picea glauca, Pinus edulis, Pinus elliottii, Pinus resinosa, Populus balsamifera, and Quercus alba) were then compared and contrasted. Lateral roots 75% of the total number and length of individual roots sampled in all species except Liriodendron tulipifera. Both SRL and N concentration decreased with increasing root order in all nine species, and this pattern appears to be universal in all temperate and boreal trees. Nitrogen concentrations ranged from 8.5 to 30.9 g/kg and were highest in the first-order "root tips." On a mass basis, first- order roots are expensive to maintain per unit time (high tissue N concentration). Tissue N appears to be a key factor in understanding the C cost of maintaining first- and second- order roots, which dominate the display of absorbing root length. There were many sig- nificant differences among species in diameter, length, SRL, and N concentration. For example, two different species can have similar SRL but very different tissue N concen- trations. Our findings run contrary to the common idea that all roots of a given size class function the same way and that a common size class for fine roots works well for all species. Interestingly, fine root lateral branches are apparently deciduous, with a distinct lateral branch scar. The position of an individual root on the branching root system appears to be important in understanding the function of fine roots.

A Finite Element Model of the Human Knee Joint for the Study of Tibio-Femoral Contact

Abstract: As a step towards developing a finite element model of the knee that can be used to study how the variables associated with a meniscal replacement affect tibio-femoral contact, the goals of this study were 1) to develop a geometrically accurate three-dimensional solid model of the knee joint with special attention given to the menisci and articular cartilage, 2) to determine to what extent bony deformations affect contact behavior, and 3) to determine whether constraining rotations other than flexion/extension affects the contact behavior of the joint during compressive loading. The model included both the cortical and trabecular bone of the femur and tibia, articular cartilage of the femoral condyles and tibial plateau, both the medial and lateral menisci with their horn attachments, the transverse ligament, the anterior cruciate ligament, and the medial collateral ligament. The solid models for the menisci and articular cartilage were created from surface scans provided by a noncontacting, laser-based, three-dimensional coordinate digitizing system with an root mean squared error (RMSE) of less than 8 microns. Solid models of both the tibia and femur were created from CT images, except for the most proximal surface of the tibia and most distal surface of the femur which were created with the three-dimensional coordinate digitizing system. The constitutive relation of the menisci treated the tissue as transversely isotropic and linearly elastic. Under the application of an 800 N compressive load at 0 degrees of flexion, six contact variables in each compartment (ie., medial and lateral) were computed including maximum pressure, mean pressure, contact area, total contact force, and coordinates of the center of pressure. Convergence of the finite element solution was studied using three mesh sizes ranging from an average element size of 5 mm by 5 mm to 1 mm by 1 mm. The solution was considered converged for an average element size of 2 mm by 2 mm. Using this mesh size, finite element solutions for rigid versus deformable bones indicated that none of the contact variables changed by more than 2% when the femur and tibia were treated as rigid. However, differences in contact variables as large as 19% occurred when rotations other than flexion/extension were constrained. The largest difference was in the maximum pressure. Among the principal conclusions of the study are that accurate finite element solutions of tibio-femoral contact behavior can be obtained by treating the bones as rigid. However, unrealistic constraints on rotations other than flexion/extension can result in relatively large errors in contact variables.

Snowmobile activity and glucocorticoid stress responses in wolves and elk

Abstract: The effect of human activities on animal populations is widely debated, particularly since a recent decision by the U.S. Department of the Interior to ban snowmobiles from national parks. Immunoassays of fecal glucocorticoid levels provide a sensitive and noninvasive method of measuring the physiological stress responses of wildlife to disturbances. We tested for associations between snowmobile activity and glucocorticoid levels in an elk (Cervus elaphus) population in Yellowstone National Park and wolf (Canis lupus) populations in Yellowstone, Voyageurs, and Isle Royale national parks. For wolves, comparisons among populations and years showed that fecal glucocorticoid levels were higher in areas and times of heavy snowmobile use. For elk, day-to-day variation in fecal glucocorticoid levels paralleled variation in the number of snowmobiles after we controlled for the effects of weather and age. Also for elk, glucocorticoid concentrations were higher in response to snowmobiles than to wheeled vehicles after we controlled for the effects of age, weather, and number of vehicles. Despite these stress responses, there was no evidence that current levels of snowmobile activity are affecting the population dynamics of either species in these locations.

Below‐ground carbon input to soil is controlled by nutrient availability and fine root dynamics in loblolly pine

Abstract: Summary • Availability of growth limiting resources may alter root dynamics in forest ecosystems, possibly affecting the land–atmosphere exchange of carbon. This was evaluated for a commercially important southern timber species by installing a factorial experiment of fertilization and irrigation treatments in an 8-yr-old loblolly pine (Pinus taeda) plantation. • After 3 yr of growth, production and turnover of fine, coarse and mycorrhizal root length was observed using minirhizotrons, and compared with stem growth and foliage development. • Fertilization increased net production of fine roots and mycorrhizal roots, but did not affect coarse roots. Fine roots had average lifespans of 166 d, coarse roots 294 d and mycorrhizal roots 507 d. Foliage growth rate peaked in late spring and declined over the remainder of the growing season, whereas fine roots experienced multiple growth flushes in the spring, summer and fall. • We conclude that increased nutrient availability might increase carbon input to soils through enhanced fine root turnover. However, this will depend on the extent to which mycorrhizal root formation is affected, as these mycorrhizal roots have much longer average lifespans than fine and coarse roots.

Altered performance of forest pests under atmospheres enriched by CO2 and O3.

Abstract: Human activity causes increasing background concentrations of the greenhouse gases C02 and O3. Increased levels of C02 can be found in all terrestrial ecosystems. Damaging O3 concentrations currently occur over 29% of the world's temperate and subpolar forests but are predicted to affect fully 60% by 2100 (ref. 3). Although individual effects of C02 and O3, on vegetation have been widely investigated, very little is known about their interaction, and long-term studies on mature trees and higher trophic levels are extremely rare. Here we present evidence from the most widely distributed North American tree species, Populus tremuloides, showing that C02 and O3, singly and in combination, affected productivity, physical and chemical leaf defences and, because of changes in plant quality, insect and disease populations. Our data show that feedbacks to plant growth from changes induced by C02 and O3 in plant quality and pest performance are likely. Assessments of global change effects on forest ecosystems must therefore consider the interacting effects of C02 and O3 on plant performance, as well as the implications of increased pest activity.

Root respiration in North American forests: effects of nitrogen concentration and temperature across biomes

Abstract: Root respiration rates have been shown to be correlated with temperature and root N concentration in studies of individual forest types or species, but it is not known how universal these relationships are across for- est species adapted to widely different climatic and eda- phic conditions. In order to test for broad, cross-species relationships, we measured fine root respiration, as O 2 consumption, over a range of temperatures on excised root samples from ten forested study sites across North America in 1997. Significant differences existed among study sites in root respiration rates, with patterns among sites in respiration rate at a given temperature corre- sponding to differences among sites in fine root N con- centrations. Root respiration rates were highly correlated with root N concentrations at all measurement tempera- tures (r 2 >0.81, P<0.001, for 6, 18 and 24°C). Lower root respiration rates in gymnosperms than in angiosperms were largely explained by lower fine root N concentra- tions in gymnosperms, and root N concentrations and respiration rates (at a given temperature) tended to be lower at warm sites (New Mexico, Florida, and Georgia) than at cool sites with short growing seasons (Michigan and Alaska). Root respiration rates increased exponen- tially with temperature at all sites. The Q10 for root respi- ration ranged from 2.4 to 3.1, but there were no signifi- cant differences among the forest types. The average Q10s for gymnosperms (Q10=2.7) and angiosperms (Q 10 =2.6) were almost identical, as were the average Q 10 s for roots of ectomycorrhizal species ( Q 10 =2.7) and arbuscular mycorrhizal species (Q10=2.6). In 1998, fine root respiration at the study sites was measured in the field as CO 2 production at ambient soil temperature. Respiration rates under field conditions were dependent on both ambient soil temperature and root N concentra- tion. Relationships between respiration (adjusted for temperature) and root N concentration for the field mea- surements were similar to those observed in the 1997 laboratory experiments. For root respiration in tree spe- cies, it appears that basic relationships with temperature and nitrogen exist across species and biomes.

Global optimization by energy landscape paving.

Abstract: We introduce a novel heuristic global optimization method, energy landscape paving (ELP), which combines core ideas from energy surface deformation and tabu search. In appropriate limits, ELP reduces to existing techniques. The approach is very general and flexible and is illustrated here on two protein folding problems. For these examples, the technique gives faster convergence to the global minimum than previous approaches.

Human muscle sympathetic neural and haemodynamic responses to tilt following spaceflight.

Abstract: Orthostatic intolerance is common when astronauts return to Earth: after brief spaceflight, up to two-thirds are unable to remain standing for 10 min. Previous research suggests that susceptible individuals are unable to increase their systemic vascular resistance and plasma noradrenaline concentrations above pre-flight upright levels. In this study, we tested the hypothesis that adaptation to the microgravity of space impairs sympathetic neural responses to upright posture on Earth. We studied six astronauts approximately 72 and 23 days before and on landing day after the 16 day Neurolab space shuttle mission. We measured heart rate, arterial pressure and cardiac output, and calculated stroke volume and total peripheral resistance, during supine rest and 10 min of 60 deg upright tilt. Muscle sympathetic nerve activity was recorded in five subjects, as a direct measure of sympathetic nervous system responses. As in previous studies, mean (+/- S.E.M.) stroke volume was lower (46 +/- 5 vs. 76 +/- 3 ml, P = 0.017) and heart rate was higher (93 +/- 1 vs. 74 +/- 4 beats min(-1), P = 0.002) during tilt after spaceflight than before spaceflight. Total peripheral resistance during tilt post flight was higher in some, but not all astronauts (1674 +/- 256 vs. 1372 +/- 62 dynes s cm(-5), P = 0.32). No crew member exhibited orthostatic hypotension or presyncopal symptoms during the 10 min of postflight tilting. Muscle sympathetic nerve activity was higher post flight in all subjects, in supine (27 +/- 4 vs. 17 +/- 2 bursts min(-1), P = 0.04) and tilted (46 +/- 4 vs. 38 +/- 3 bursts min(-1), P = 0.01) positions. A strong (r(2) = 0.91-1.00) linear correlation between left ventricular stroke volume and muscle sympathetic nerve activity suggested that sympathetic responses were appropriate for the haemodynamic challenge of upright tilt and were unaffected by spaceflight. We conclude that after 16 days of spaceflight, muscle sympathetic nerve responses to upright tilt are normal.

The effect of prey and predator densities on wolf predation

Abstract: Predator kills rate (i.e., kills per predator per time) is routinely presupposed to depend exclusively on prey density. However, per capita rates of killing may typically depend on the density of both prey and predator. Unfortunately, our perception of many ecological phenomena may be limited by the inappropriate assumption that kill rates do not depend on predator density. One of many ways to represent the influence of predator density is ratio-dependent predation, where kill rate depends on the ratio of prey to predator rather than the actual numbers of prey and predator. Determining the role of ratio dependency in predation theory has been contentious. Assessments of the influence of predator density on kill rate have been primarily limited to theoretical considerations, indirect evidence, and simplified laboratory demonstrations. We directly observed the influence of both prey and predator density on kill rates in an unmanipulated terrestrial system of large mammals— wolves ( Canis lupus) and moose (Alces alces). Predator density explained more variation in kill rate than did prey density (R 2 5 0.36 vs. R 2 5 0.17, respectively). Moreover, the ratio-dependent model greatly outperformed the prey-dependent model. Nevertheless, the ratio-dependent model failed to explain most of the variation in kill rate (i.e., R 2 5 0.34). The ratio-dependent-prey-dependent controversy may dissipate with greater appreciation and acknowledgment that both models may be overly simplistic, both have value, and neither deserves primacy.

Evaluation of electrical conductivity models for conductive polymer composites

Abstract: The electrical conductivity of polymeric materials can be increased by the addition of carbon fillers, such as carbon fibers and graphite. The resulting composites could be used in applications such as interference shielding and electrostatic dissipation. Electrical conductivity models are often proposed to predict the conductivity behavior of these materials in order to achieve more efficient material design that could reduce costly experimental work. The electrical conductivity of carbon-filled polymers was studied by adding four single fillers to nylon 6,6 and polycarbonate in increasing concentrations. The fillers used in this project include chopped and milled forms of polyacrylonitrile (PAN) carbon fiber, ThermocarbTM Specialty Graphite, and Ni-coated PAN carbon fiber. Material was extruded and injection-molded into test specimens, and then the electrical conductivity was measured. Data analysis included a comparison of the results to existing conductivity models. The results show that the model proposed by Mamunya, which takes into account the filler aspect ratio and the surface energy of the filler and polymer, most closely matched the conductivity data. This information will then be used in the development of improved conductivity models. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 83: 1341–1356, 2002

Differential Expression of Two Distinct Phenylalanine Ammonia-Lyase Genes in Condensed Tannin-Accumulating and Lignifying Cells of Quaking Aspen

Abstract: Lignins, along with condensed tannins (CTs) and salicylate-derived phenolic glycosides, constitute potentially large phenylpropanoid carbon sinks in tissues of quaking aspen (Populus tremuloides Michx.). Metabolic commitment to each of these sinks varies during development and adaptation, and depends on L-phenylalanine ammonia-lyase (PAL), an enzyme catalyzing the deamination of L-phenylalanine to initiate phenylpropanoid metabolism. In Populus spp., PAL is encoded by multiple genes whose expression has been associated with lignification in primary and secondary tissues. We now report cloning two differentially expressed PAL cDNAs that exhibit distinct spatial associations with CT and lignin biosynthesis in developing shoot and root tissues of aspen. PtPAL1 was expressed in certain CT-accumulating, non-lignifying cells of stems, leaves, and roots, and the pattern of PtPAL1 expression varied coordinately with that of CT accumulation along the primary to secondary growth transition in stems. PtPAL2 was expressed in heavily lignified structural cells of shoots, but was also expressed in non-lignifying cells of root tips. Evidence of a role for Pt4CL2, encoding 4-coumarate:coenzyme A ligase, in determining CT sink strength was gained from cellular co-expression analysis with PAL1 and CTs, and from experiments in which leaf wounding increased PAL1 and 4CL2 expression as well as the relative allocation of carbon to CT with respect to phenolic glycoside, the dominant phenolic sink in aspen leaves. Leaf wounding also increased PAL2 and lignin pathway gene expression, but to a smaller extent. The absence of PAL2 in most CT-accumulating cells provides in situ support for the idea that PAL isoforms function in specific metabolic milieus.

Performance evaluation of track fusion with information matrix filter

Abstract: In a multisensor environment, each sensor detects multiple targets and creates corresponding tracks. Fusion of tracks from these, possibly dissimilar, sensors yields more accurate kinematic and attribute information regarding the target. Two methodologies have been employed for such purpose, which are: measurement fusion and state vector fusion. It is well known that the measurement fusion approach is optimal but computationally inefficient and the state vector fusion algorithms are more efficient but suboptimal, in general. This is so because the state vector estimates to be fused obtained from two sensors, are not conditionally independent in general due to the common process noise from the target being tracked. It is to be noted that there are three approaches to state vector fusion, which are: weighted covariance, information matrix, and pseudomeasurement. This research is restricted solely to performance evaluation of the information matrix form of state vector fusion. Closed-form analytical solution of steady state fused covariance has been derived as a measure of performance using this approach. Note that the results are derived under the assumptions that the two sensors are synchronized and no misassociation or merged measurement is considered in the study. Results are compared with those using Monte Carlo simulation, which was used in the past to predict fusion system performance by various authors. These results provide additional insight into the mechanism of track fusion and greatly simplify evaluation of fusion performance. In addition, availability of such a solution facilitates the trade-off studies for designing fusion systems under various operating conditions.

Tephra fallout in the eruption of Soufrière Hills Volcano, Montserrat

Abstract: Four mechanisms caused tephra fallout at Soufriere Hills Volcano, Montserrat, during the 1995-1999 period: explosive activity (mainly of Vulcanian type), dome collapses, ash-venting and phreatic explosions. The first two mechanisms contributed most of the tephra-fallout deposits (minimum total dense-rock equivalent volume of 23 x 10 6 m 3 ), which vary from massive to layered and represent the amalgamation of the deposits from a large numbers of events. The volume of co-pyroclastic-flow fallout tephra is in the range 4-16° of the associated pyroclastic flow deposits. Dome-collapse fallout tephra is characterized by ash particles generated by fragmentation in the pyroclastic flows and by elutriation of fines. Vulcanian fallout tephra is coarser grained, as it is formed by magma fragmentation in the conduit and by elutriation from the fountain-collapse flows and initial surges. Vulcanian fallout tephra is typically polymodal, whereas dome-collapse fallout tephra is predominantly unimodal. Polymodality is attributed to: overlapping of fallout tephra of different types, premature fallout of fine particles, multiple tephra-fallout sources, and differences in density and grain-size distribution of different components. During both dome collapses and explosions, ash fell as aggregates of various sizes and types. Accretionary lapilli grain size is independent of their diameter and is characterized by multiple subpopulations with a main mode at 5o. Satellite data indicate that very fine ash can stay in a volcanic cloud for several hours and show that exponential thinning rates observed in proximal areas cannot apply in distal areas.

Influence of Exotic Earthworms on the Soil Organic Horizon and the Rare Fern Botrychium mormo

Abstract: Forests north of the last glacial extent have no native earthworms. Exotic earthworms are now colonizing forests that are naturally free of earthworms. It is currently unknown how these exotic earthworms might affect rare plants. To determine whether there is an association between the presence of an exotic earthworm species and extirpation of the rare fern Botrychium mormo, I surveyed 28 populations documented and counted previously. I estimated current population sizes of B. mormo, soil horizon thickness, earthworm species present, and carbon content, nitrogen content, and pH of the A soil horizon. Two earthworm species were abundant, Lumbricus rubellus and Dendrobaena octaedra. Dendrobaena octaedra had no significant association with any soil variable or with B. mormo extirpation. Lumbricus rubellus was significantly associated with B. mormo extirpation and a mull humus type. Where L. rubellus was present, O1 and O2 horizons were significantly thinner. I conducted a laboratory microcosm experiment to determine whether L. rubellus could create the conditions it was associated with in the field. Microcosms with L. rubellus resulted in a significant reduction in the thickness of the O1 and O2 horizon and a significant increase in the thickness of the A horizon. This experiment suggests that L. rubellus created the conditions with which it was associated in the field. The intrinsic rate of increase (r) of B. mormo was best explained by the O2 depth, which implies that this soil layer supplies a critical resource. My results support the idea that exotic earthworms alter the forest floor, leading to negative changes in native vegetation.

Association mapping, using a mixture model for complex traits

Abstract: Association mapping for complex diseases using unrelated individuals can be more powerful than family-based analysis in many settings. In addition, this approach has major practical advantages, including greater efficiency in sample recruitment. Association mapping may lead to false-positive findings, however, if population stratification is not properly considered. In this paper, we propose a method that makes it possible to infer the number of subpopulations by a mixture model, using a set of independent genetic markers and then testing the association between a genetic marker and a trait. The proposed method can be effectively applied in the analysis of both qualitative and quantitative traits. Extensive simulations demonstrate that the method is valid in the presence of a population structure.

Characterization of uniaxial compressive response of bulk amorphous Zr–Ti–Cu–Ni–Be alloy

Abstract: Uniaxial compressive response of bulk amorphous Zr–Ti–Cu–Ni–Be alloy, also called as Vitreloy-1, was investigated at quasistatic and high strain rates in the range of 10−3 and 103 s−1, respectively. The Vitreloy-1 specimens exhibited elastic response followed by catastrophic fracture along a narrow shear band. The ultimate strength of the specimens varied between 1800 and 2200 MPa irrespective of the strain rate and independent of the aspect ratio of the specimens. The quasistatically deformed specimens fractured into two or three large fragments. The fracture surfaces were relatively smooth and revealed well developed and uniformly distributed veinal pattern. The dynamically loaded specimens, on the other hand, fractured into several fragments with relatively rough fracture surfaces containing nonuniformly distributed and partially developed veinal patterns. Evidence of melting in the form of ‘liquid bubbles’ was also observed along the cracks on the fracture surfaces of the specimens subjected to high strain-rate loading. A comparison of the mechanical response of Vitreloy-1 with other bulk metallic glass systems is also presented.

Accelerated ultraviolet weathering of PVC/wood‐flour composites

Abstract: Ultraviolet weathering performance of polyvinyl chloride (PVC) filled with different concentrations of wood flour was studied. Extruded PVC/wood-flour composite samples were subjected to cyclic ultraviolet lamps/condensation exposures and assessed over a total of 400 and 2600 hours. Each assessment consisted of DRIFT-FTIR and XPS collections, contact angle measurement, color measurement, and tensile property testing. The experimental results indicated that wood flours are effective chromophore materials since their incorporation into a rigid PVC matrix accelerated the degradation of the polymeric matrix. Photodegradation converted unfilled PVC samples to a colored material of lower extensibility. Although composite samples exhibited greater discoloration than unfilled PVC samples, they retained all their original strength and stiffness properties even after 2600 hours of cyclic UV irradiation/condensation exposures.

On the state of strength-three covering arrays

Abstract: A covering array of sizeN, strengtht, degree k, and order υ is a k × N array on υ symbols in which every t × N subarray contains every possible t × 1 column at least once. We present explicit constructions, constructive upper bounds on the size of various covering arrays, and compare our results with those of a commercial product. Applications of covering arrays include software testing, drug screening, and data compression. © 2002 Wiley Periodicals, Inc. J Combin Designs 10: 217–238, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jcd.10002

Differential substrate inhibition couples kinetically distinct 4-coumarate:coenzyme a ligases with spatially distinct metabolic roles in quaking aspen.

Abstract: 4-Coumarate:coenzyme A ligase (4CL) activates hydroxycinnamates for entry into phenylpropanoid branchways that support various metabolic activities, including lignification and flavonoid biosynthesis. However, it is not clear whether and how 4CL proteins with their broad substrate specificities fulfill the specific hydroxycinnamate requirements of the branchways they supply. Two tissue-specific 4CLs, Pt4CL1 and Pt4CL2, have previously been cloned from quaking aspen (Populus tremuloides Michx.), but whether they are catalytically adapted for the distinctive metabolic roles they are thought to support is not apparent from published biochemical data. Therefore, single- and mixed-substrate assays were conducted to determine whether the 4CLs from aspen exhibit clear catalytic identities under certain metabolic circumstances. Recombinant Pt4CL1 and Pt4CL2 exhibited the expected preference for p-coumarate in single-substrate assays, but strong competitive inhibition favored utilization of caffeate and p-coumarate, respectively, in mixed-substrate assays. The Pt4CL1 product, caffeoyl-CoA, predominated in mixed-substrate assays with xylem extract, and this was consistent with the near absence of Pt4CL2 expression in xylem tissue as determined by in situ hybridization. It is interesting that the Pt4CL2 product p-coumaroyl-CoA predominated in assays with developing leaf extract, although in situ hybridization revealed that both genes were coexpressed. The xylem extract and recombinant 4CL1 data allow us to advance a mechanism by which 4CL1 can selectively utilize caffeate for the support of monolignol biosynthesis in maturing xylem and phloem fibers. Loblolly pine (Pinus taeda), in contrast, possesses a single 4CL protein exhibiting broad substrate specificity in mixed-substrate assays. We discuss these 4CL differences in terms of the contrasts in lignification between angiosperm trees and their gymnosperm progenitors.