Showing papers by "University of Hamburg published in 1992"

The skeletal muscle chloride channel in dominant and recessive human myotonia.

Abstract: Autosomal recessive generalized myotonia (Becker's disease) (GM) and autosomal dominant myotonia congenita (Thomsen's disease) (MC) are characterized by skeletal muscle stiffness that is a result of muscle membrane hyperexcitability. For both diseases, alterations in muscle chloride or sodium currents or both have been observed. A complementary DNA for a human skeletal muscle chloride channel (CLC-1) was cloned, physically localized on chromosome 7, and linked to the T cell receptor beta (TCRB) locus. Tight linkage of these two loci to GM and MC was found in German families. An unusual restriction site in the CLC-1 locus in two GM families identified a mutation associated with that disease, a phenylalanine-to-cysteine substitution in putative transmembrane domain D8. This suggests that different mutations in CLC-1 may cause dominant or recessive myotonia.

Fennoscandian summers from ad 500: temperature changes on short and long timescales

Abstract: Quantitative estimates of 1480 years of summer temperatures in northern Fennoscandia have previously been derived from continuous treering records from northern Sweden. Here we show the results of spectral analyses of these data. Only a few peaks in the spectra are consistently significant when the data are analyzed over a number of sub-periods. Relatively timestable peaks are apparent at periods of 2.1, 2.5, 3.1, 3.6, 4.8, ∼ 32–33 and for a range between ∼ 55–100 years. These results offer no strong evidence for solar-related forcing of summer temperatures in these regions. Our previously published reconstruction was limited in its ability to represent long-timescale temperature change because of the method used to standardize the original tree-ring data. Here we employ an alternative standardization technique which enables us to capture temperature change on longer timescales. Considerable variance is now reconstructed on timescales of several centuries. In comparison with modern normals (1951–70) generally extended periods when cool conditions prevailed, prior to the start of the instrumental record, include 500–700, 790–870, 1110–1150, 1190–1360, 1570–1750 (A.D.) with the most significant cold troughs centred on about 660, 800, 1140, 1580–1620 and 1640. Predominantly warm conditions occurred in 720–790, 870–1110 and 1360–1570 with peaks of warmth around 750, 930, 990, 1060, 1090, 1160, 1410, 1430, 1760 and 1820.

Using Orientation Information for Qualitative Spatial Reasoning

Abstract: A new approach to representing qualitative spatial knowledge and to spatial reasoning is presented This approach is motivated by cognitive considerations and is based on relative orientation information about spatial environments The approach aims at exploiting properties of physical space which surface when the spatial knowledge is structured according to conceptual neighborhood of spatial relations The paper introduces the notion of conceptual neighborhood and its relevance for qualitative temporal reasoning The extension of the benefits to spatial reasoning is suggested Several approaches to qualitative spatial reasoning are briefly reviewed Differences between the temporal and the spatial domain are outlined A way of transferring a qualitative temporal reasoning method to the spatial domain is proposed The resulting neighborhood-oriented representation and reasoning approach is presented and illustrated An example for an application of the approach is discussed

A chloride channel widely expressed in epithelial and non-epithelial cells.

Abstract: Chloride channels have several functions, including the regulation of cell volume, stabilizing membrane potential, signal transduction and transepithelial transport. The plasma membrane Cl- channels already cloned belong to different structural classes: ligand-gated channels, voltage-gated channels, and possibly transporters of the ATP-binding-cassette type (if the cystic fibrosis transmembrane regulator is a Cl- channel). The importance of chloride channels is illustrated by the phenotypes that can result from their malfunction: cystic fibrosis, in which transepithelial transport is impaired, and myotonia, in which ClC-1, the principal skeletal muscle Cl- channel, is defective. Here we report the properties of ClC-2, a new member of the voltage-gated Cl- channel family. Its sequence is approximately 50% identical to either the Torpedo electroplax Cl- channel, ClC-0 (ref. 8), or the rat muscle Cl- channel, ClC-1 (ref. 9). Isolated initially from rat heart and brain, it is also expressed in pancreas, lung and liver, for example, and in pure cell lines of fibroblastic, neuronal, and epithelial origin, including tissues and cells affected by cystic fibrosis. Expression in Xenopus oocytes induces Cl- currents that activate slowly upon hyperpolarization and display a linear instantaneous current-voltage relationship. The conductivity sequence is Cl- greater than or equal to Br- greater than I-. The presence of ClC-2 in such different cell types contrasts with the highly specialized expression of ClC-1 (ref. 9) and also with the cloned cation channels, and suggests that its function is important for most cells.

Distribution of ADH2 and ALDH2 genotypes in different populations

Abstract: The distribution of the human liver alcohol dehydrogenase, ADH2, and aldehyde dehydrogenase, ALDH2, genotypes in 21 different populations comprising Mongoloids, Caucasoids, and Negroids was determined by hybridization of the amplified genomic DNA with allele-specific oligonucleotide probes. Whereas the frequency of the ADH1(2) allele was found to be relatively high in the Caucasoids, Mexican Mestizos, Brazilian Indios, Swedish Lapps, Papua New Guineans and Negroids, the frequency of the ADH2(2) gene was considerably higher in the Mongoloids and Australian Aborigines. The atypical ALDH2 gene (ALDH2(2)) was found to be extremely rare in Caucasoids, Negroids, Papua New Guineans, Australian Aborigines and Aurocanians (South Chile). In contrast, this mutant gene was found to be widely prevalent among the Mongoloids. Individuals possessing the abnormal ALDH2 gene show alcohol-related sensitivity responses (e.g. facial flushing), have the tendency not to be habitual drinkers, and apparently suffer less from alcoholism and alcohol-related liver disease.

Regions involved in the opening of CIC-2 chloride channel by voltage and cell volume.

Abstract: Regulation of cell volume is essential for every cell and is accomplished by the regulated loss or gain of intracellular ions or other osmolytes. Regulatory volume decrease often involves the parallel activation of potassium and chloride channels. Overexpression of P-glycoprotein leads to volume-activated Cl- currents but its physiological importance for volume regulation is unclear. CIC-2 is a ubiquitously expressed Cl- channel activatable by non-physiologically strong hyperpolarization. We now show that CIC-2 can be activated by extracellular hypotonicity, which suggests that it has a widespread role in volume regulation. Domains necessary for activation by both voltage and volume are localized to the amino terminus. Mutations in an 'essential' region lead to constitutively open channels unresponsive to medium tonicity, whereas deletions in a 'modulating' region produce partially opened channels responsive to both hypo- and hypertonicity. These domains can be transplanted to different regions of the protein without loss of function.

Role of octreotide in the prevention of postoperative complications following pancreatic resection.

Abstract: Though morbidity and mortality rates following pancreatic resection have improved in recent years, they are still around 35% and 5%, respectively. Typical complications, such as pancreatic fistula, abscess, and subsequent sepsis, are chiefly associated with exocrine pancreatic secretion. In order to clarify whether the perioperative inhibition of exocrine pancreatic secretion prevents complications, we assessed the efficacy of octreotide, a long-acting somatostatin analogue. We conducted a randomized, double-blind, placebo-controlled, multicenter trial in 246 patients undergoing major elective pancreatic surgery. Patients were stratified into a high-risk stratum (limited to patients with pancreatic and periampullary tumors) or low-risk stratum (patients with chronic pancreatitis). Patients received octreotide (3 x 100 micrograms) or placebo subcutaneously for 7 days perioperatively. Eleven complications were defined: death, leakage of anastomosis, pancreatic fistula, abscess, fluid collection, shock, sepsis, bleeding, pulmonary insufficiency, renal insufficiency, and postoperative pancreatitis. Two hundred patients underwent pancreatic head resection, 31 patients underwent left resection, and 15 patients had other procedures. The overall mortality rate within 90 days was 4.5%, with 3.2% in the octreotide group and 5.8% in the placebo group. The complication rate was 32% in the patients receiving octreotide (40 of 125 patients) and 55% in patients receiving placebo (67 of 121 patients) (p less than 0.005). In the patients in the high-risk stratum, complications were observed in 26 of the 68 (38%) patients treated with octreotide and in 46 of 71 (65%) patients given placebo (p less than 0.01). Whereas in patients in the low-risk stratum, the complication rate was 25% (14 of 57 patients) in those treated with octreotide and 42% (21 of 50 patients) in patients given placebo (p = NS). The perioperative application of octreotide reduces the occurrence of typical postoperative complications after pancreatic resection, particularly in patients with tumors.

Soft tissue reactions to non-submerged unloaded titanium implants in beagle dogs.

Abstract: The soft tissue reactions to non-submerged unloaded titanium implants were examined. A total of 24 implants were placed in 6 beagle dogs. The implants differed in their crestal area by having either a rough sandblasted, a fine sandblasted, or a polished surface. After 3 months, all implants were firmly anchored in the bone and had no clinical signs of peri-implant inflammation. Undecalcified histologic sections demonstrated that all implants achieved osseointegration with direct bone contact. The epithelial structures showed a peri-implant sulcus with a non-keratinized sulcular epithelium and a junctional epithelium. None of the sections exhibited epithelial downgrowth to the alveolar crest. In the supracrestal area, a direct connective tissue contact to the implant post was observed. An approximately 50 to 100 microns wide zone of dense circular fibers was found close to the implant surface. It was free of blood vessels and resembled closely an inflammation-free scar tissue formation. This zone was surrounded by a looser connective tissue with a 3-dimensional network of collagen fibers running in different directions. No significant differences concerning soft tissue reactions were found between the 3 implant surfaces. In particular, the length of direct connective tissue contact was similar. Concerning bone reactions, a significantly shorter distance from the top of the implant to the most coronal bone-implant contact was observed for rough surfaces. It is concluded that non-submerged unloaded titanium implants achieved a complication-free tissue integration with a dense connective tissue in direct contact to the implant surface in the supracrestal area, and epithelial structures similar to those around natural teeth. The different surface textures did not influence the healing pattern of the soft tissues, but had an influence on the location of the most coronal bone-implant contact.

The World Health Organization's Histological Classification of Salivary Gland Tumors. A commentary on the second edition.

Abstract: The second edition of the World Health Organization's Histological Classification of Salivary Gland Tumors is more extensive and detailed than the previous edition published 20 years ago. The new edition is based on data regarding newly described tumor entities and the behavior and prognosis of the previously classified tumors. The distinct morphologic features of monomorphic adenomas justify their separation for purposes of identification. Among the carcinomas, various types were distinguished for purposes of recognition, prognosis, and treatment. The term tumor was replaced by carcinoma in the following two entities: acinic cell carcinoma and mucoepidermoid carcinoma. The tumor-like lesions were described in more detail.

Spin-singlet-spin-triplet oscillations in quantum dots

Abstract: Two interacting electrons confined to a disk on a semiconductor surface are considered in a perpendicular magnetic field. As it is appropriate for experimental realizations, we use a two-dimensional harmonic-oscillator well to confine the electrons in the plane of the disk. We predict oscillations between spin-singlet and spin-triplet ground states as a function of the magnetic field strnegth. Phase diagrams describing this peculiar manifestation of the electron-electron interaction in a quantum dot are calculated for GaAs and experiments to verify them are proposed.

Ion transport anomalies in glasses.

Abstract: A model for ion transport in glass is presented which is based on the experimental evidence that cations in glass create and maintain their own characteristic environments. By postulating the existence of a site memory effect, we are able to visualize the formation of conducting pathways. We develop a quantitative theory which explains the general occurrence of the mixed cation (alkali) erect in glassy material and, in addition, shows that the anomalous dependence of conductivity on the modifer content in single alkali glasses follows a simple power-law relation

The detector simulation program for the OPAL experiment at LEP

Abstract: The Monte Carlo program for the OPAL experiment at the LEP ee collider is described. This program is based on the GEANT simulation package. The general organization of the program is outlined, and a description is given of the techniques used for simulating the various subdetectors of OPAL. The performance of the program is illustrated by comparisons with recent data recorded by OPAL at LEP. (Submitted to Nucl. Instr. and Meth.) School of Physics and Space Research, University of Birmingham, Birmingham, B15 2TT, UK Dipartimento di Fisica dell' Universit a di Bologna and INFN, Bologna, 40126, Italy CNAF-INFN, Bologna, Italy Physikalisches Institut, Universitat Bonn, D-5300 Bonn 1, FRG Department of Physics, University of California, Riverside, CA 92521 USA Cavendish Laboratory, Cambridge, CB3 0HE, UK Carleton University, Dept of Physics, Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada Centre for Research in Particle Physics, Carleton University, Ottawa, Ontario K1S 5B6, Canada CERN, European Organisation for Particle Physics, 1211 Geneva 23, Switzerland Enrico Fermi Institute and Department of Physics, University of Chicago, Chicago Illinois 60637, USA Fakult at f ur Physik, Albert Ludwigs Universitat, D-7800 Freiburg, FRG Universitat Hamburg/DESY, II Inst. f ur Experimental Physik, 2000 Hamburg 52, FRG Physikalisches Institut, Universitat Heidelberg, Heidelberg, FRG Queen Mary and West eld College, University of London, London, E1 4NS, UK University College London, London, WC1E 6BT, UK Department of Physics, Schuster Laboratory, The University, Manchester, M13 9PL, UK Department of Physics and Astronomy, University of Maryland, College Park, Maryland 20742, USA Laboratoire de Physique Nucl eaire, Universit e de Montr eal, Montr eal, Quebec, H3C 3J7, Canada Rutherford Appleton Laboratory, Chilton, Didcot, Oxfordshire, OX11 0QX, UK DPhPE, CEN Saclay, F-91191 Gif-sur-Yvette, France International Centre for Elementary Particle Physics, University of Tokyo, Tokyo 113, Japan Brunel University, Uxbridge, Middlesex, UB8 3PH UK Nuclear Physics Department, Weizmann Institute of Science, Rehovot, 76100, Israel Present address: EPFL, Lausanne, Switzerland Present address: Applied Silicon Inc, Ottawa, Canada Present address: Rutherford Appleton Laboratory, Chilton, Didcot, Oxfordshire, OX11 0QX, UK On leave from Birmingham University, Birmingham B15 2TT, UK Present address: Culham Laboratory, Culham, Oxfordshire, UK Present address: Meiji Gakuin University, Yokohama 244, Japan

Metabolism of dibenzo-p-dioxin by Sphingomonas sp. strain RW1.

Abstract: In the course of our screening for dibenzo-p-dioxin-utilizing bacteria, a Sphingomonas sp. strain was isolated from enrichment cultures inoculated with water samples from the river Elbe. The isolate grew with both the biaryl ethers dibenzo-p-dioxin and dibenzofuran (DF) as the sole sources of carbon and energy, showing doubling times of about 8 and 5 h, respectively. Biodegradation of the two aromatic compounds initially proceeded after an oxygenolytic attack at the angular position adjacent to the ether bridge, producing 2,2',3-trihydroxydiphenyl ether or 2,2',3-trihydroxybiphenyl from the initially formed dihydrodiols, which represent extremely unstable hemiacetals. Results obtained from determinations of enzyme activities and oxygen consumption suggest meta cleavage of the trihydroxy compounds. During dibenzofuran degradation, hydrolysis of 2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate yielded salicylate, which was branched into the catechol meta cleavage pathway and the gentisate pathway. Catechol obtained from the product of meta ring fission of 2,2',3-trihydroxydiphenyl ether was both ortho and meta cleaved by Sphingomonas sp. strain RW1 when this organism was grown with dibenzo-p-dioxin.

The crystal barrel spectrometer at LEAR

Abstract: The crystal Barrel spectrometer used at LEAR, CERN to study the products of pd annihilations is described. A 1380 element array of Csl crystals measures photons from the decay of π0, η, η′ and ω mesons. A segmented drift chamber in a 1.5T magnetic field is used to identify and measure charged particles. A fast on-line trigger on charged and neutral multiplicities and on the invariant mass of secondary particles is available. The performance of the detector is discussed.

Evaluation of Leptospirillum ferrooxidans for Leaching.

Abstract: The importance of Leptospirillum ferrooxidans for leach processes has been evaluated by studying the lithotrophic flora of three mine biotopes and a heap leaching operation, by percolation experiments with inoculated, sterilized ore, and by morphological, physiological, and genetic investigations of pure and mixed cultures of L. ferrooxidans, Thiobacillus ferrooxidans, and Thiobacillus thiooxidans. In biotopes of 20 degrees C or above, Leptospirillum-like bacteria are as abundant as T. ferrooxidans. Leptospirilli represent at least one-half of the ferrous-iron-oxidizing population. Percolation experiments confirmed this result. Leptospirilli were as numerous as T. ferrooxidans. At reduced temperatures, the generation times of leptospirilli increase more so than those of T. ferrooxidans. At 14 degrees C, Leptospirillum grows slowly and T. ferrooxidans dominates the population. Physiological investigations indicate that L. ferrooxidans is a strict chemolithoautotroph, metabolizing only ferrous iron and pyrite. Even an addition of 0.05% (wt/vol) yeast extract inhibited its growth. The maximum ferrous-iron-oxidizing activity of L. ferrooxidans amounts to about 40% of the activity of T. ferrooxidans. After growth on sulfidic ore, both species exhibit reduced iron-oxidizing activities, L. ferrooxidans exhibiting one-third and T. ferrooxidans exhibiting one-seventh of their maximum activities. Surprisingly, the absolute values are similar. For indirect leaching, L. ferrooxidans is as important as T. ferrooxidans. This was confirmed by the results of percolation experiments. L. ferrooxidans together with T. thiooxidans mobilized metals at least as well as T. ferrooxidans did. The best results were obtained with a mixed culture of all three species.

Increased messenger RNA level of the inhibitory G protein alpha subunit Gi alpha-2 in human end-stage heart failure.

Abstract: In human heart failure the positive inotropic and cAMP-elevating effects of both beta-adrenoceptor agonists and phosphodiesterase inhibitors are diminished. This has been explained at least in part by an increase in the inhibitory signal-transducing G protein (Gi) and unchanged stimulatory G protein (Gs). In the present study we determined the mRNA expression pattern of the alpha subunits of Gi-1, Gi-2, Gi-3, and Gs in myocardial tissue samples of patients undergoing heart transplantation. Northern blot analysis of total RNA extracted from left ventricles with 32P-labeled cDNAs demonstrated expression of Gi alpha-2, Gi alpha-3, and Gs alpha mRNA. In contrast, Gi alpha-1 mRNA was not detectable. To investigate whether the increased ratio of Gi/Gs might be due to altered gene expression, we compared mRNA levels of Gi alpha-2, Gi alpha-3, and Gs alpha in left ventricular myocardium from failing hearts with idiopathic dilated cardiomyopathy (n = 8) and ischemic cardiomyopathy (n = 6) and from nonfailing hearts from transplant donors (n = 8). Compared with nonfailing control hearts, the Gi alpha-2 mRNA was increased by 75 +/- 26% (p less than 0.05) in idiopathic dilated cardiomyopathy hearts and 90 +/- 26% (p less than 0.05) in ischemic cardiomyopathy hearts. Gi alpha-3 and Gs alpha mRNA levels were similar in the three groups. The results suggest that as in other mammalian species, Gi alpha-2 and Gi alpha-3 mRNA are the predominant Gi alpha mRNA subtypes in human ventricular myocardium.(ABSTRACT TRUNCATED AT 250 WORDS)

A comparison of ePTFE membranes alone or in combination with platelet-derived growth factors and insulin-like growth factor-I or demineralized freeze-dried bone in promoting bone formation around immediate extraction socket implants.

Abstract: The purpose of this study was to compare bone promotion around implants which were augmented with ePTFE membranes alone or in combination with cortical demineralized freeze-dried bone (DFDB) or the combination of platelet derived growth factor-BB (PDGF) and insulin like growth factor I (PDGF/IGF-I). Membranes were placed over titanium implants which had been inserted into fresh extraction sockets with large buccal dehiscences. Twenty-four implants were placed in 4 dogs. At 18 weeks clinical bone height measurements were taken, the animals were sacrificed, and all specimens retrieved for histologic evaluation. Clinically, a significant gain in bone levels was present in both the ePTFE membrane alone group (P < 0.005) and PTFE plus PDGF/IGF-I group (P <0.01), but not in the PTFE plus DFDB group. Results from histometric measurements revealed an approximately 2-fold increase in the percentage of implant surface in contact with bone, area of bone adjacent to the implant surface, and in the total length of the...

Fractional diffusion equation for transport phenomena in random media

Abstract: A differential equation for diffusion in isotropic and homogeneous fractal structures is derived within the context of fractional calculus. It generalizes the fractional diffusion equation valid in Euclidean systems. The asymptotic behavior of the probability density function is obtained exactly and coincides with the accepted asymptotic form obtained using scaling argument and exact enumeration calculations on large percolation clusters at criticality. The asymptotic frequency dependence of the scattering function is derived exactly from the present approach, which can be studied by X-ray and neutron scattering experiments on fractals.