Showing papers by "Utsunomiya University published in 2016"

Ultrafast laser processing of materials: from science to industry

Abstract: Processing of materials by ultrashort laser pulses has evolved significantly over the last decade and is starting to reveal its scientific, technological and industrial potential. In ultrafast laser manufacturing, optical energy of tightly focused femtosecond or picosecond laser pulses can be delivered to precisely defined positions in the bulk of materials via two-/multi-photon excitation on a timescale much faster than thermal energy exchange between photoexcited electrons and lattice ions. Control of photo-ionization and thermal processes with the highest precision, inducing local photomodification in sub-100-nm-sized regions has been achieved. State-of-the-art ultrashort laser processing techniques exploit high 0.1–1 μm spatial resolution and almost unrestricted three-dimensional structuring capability. Adjustable pulse duration, spatiotemporal chirp, phase front tilt and polarization allow control of photomodification via uniquely wide parameter space. Mature opto-electrical/mechanical technologies have enabled laser processing speeds approaching meters-per-second, leading to a fast lab-to-fab transfer. The key aspects and latest achievements are reviewed with an emphasis on the fundamental relation between spatial resolution and total fabrication throughput. Emerging biomedical applications implementing micrometer feature precision over centimeter-scale scaffolds and photonic wire bonding in telecommunications are highlighted.

Lateral branching oxidoreductase acts in the final stages of strigolactone biosynthesis in Arabidopsis

Abstract: Strigolactones are a group of plant compounds of diverse but related chemical structures. They have similar bioactivity across a broad range of plant species, act to optimize plant growth and development, and promote soil microbe interactions. Carlactone, a common precursor to strigolactones, is produced by conserved enzymes found in a number of diverse species. Versions of the MORE AXILLARY GROWTH1 (MAX1) cytochrome P450 from rice and Arabidopsis thaliana make specific subsets of strigolactones from carlactone. However, the diversity of natural strigolactones suggests that additional enzymes are involved and remain to be discovered. Here, we use an innovative method that has revealed a missing enzyme involved in strigolactone metabolism. By using a transcriptomics approach involving a range of treatments that modify strigolactone biosynthesis gene expression coupled with reverse genetics, we identified LATERAL BRANCHING OXIDOREDUCTASE (LBO), a gene encoding an oxidoreductase-like enzyme of the 2-oxoglutarate and Fe(II)-dependent dioxygenase superfamily. Arabidopsis lbo mutants exhibited increased shoot branching, but the lbo mutation did not enhance the max mutant phenotype. Grafting indicated that LBO is required for a graft-transmissible signal that, in turn, requires a product of MAX1. Mutant lbo backgrounds showed reduced responses to carlactone, the substrate of MAX1, and methyl carlactonoate (MeCLA), a product downstream of MAX1. Furthermore, lbo mutants contained increased amounts of these compounds, and the LBO protein specifically converts MeCLA to an unidentified strigolactone-like compound. Thus, LBO function may be important in the later steps of strigolactone biosynthesis to inhibit shoot branching in Arabidopsis and other seed plants.

Strigolactones are required for nitric oxide to induce root elongation in response to nitrogen and phosphate deficiencies in rice

Abstract: The response of the root system architecture to nutrient deficiencies is critical for sustainable agriculture. Nitric oxide (NO) is considered a key regulator of root growth, although the mechanisms remain unknown. Phenotypic, cellular and genetic analyses were undertaken in rice to explore the role of NO in regulating root growth and strigolactone (SL) signalling under nitrogen-deficient and phosphate-deficient conditions (LN and LP). LN-induced and LP-induced seminal root elongation paralleled NO production in root tips. NO played an important role in a shared pathway of LN-induced and LP-induced root elongation via increased meristem activity. Interestingly, no responses of root elongation were observed in SL d mutants compared with wild-type plants, although similar NO accumulation was induced by sodium nitroprusside (SNP) application. Application of abamine (the SL inhibitor) reduced seminal root length and pCYCB1;1::GUS expression induced by SNP application in wild type; furthermore, comparison with wild type showed lower SL-signalling genes in nia2 mutants under control and LN treatments and similar under SNP application. Western blot analysis revealed that NO, similar to SL, triggered proteasome-mediated degradation of D53 protein levels. Therefore, we presented a novel signalling pathway in which NO-activated seminal root elongation under LN and LP conditions, with the involvement of SLs.

Fairy Lights in Femtoseconds: Aerial and Volumetric Graphics Rendered by Focused Femtosecond Laser Combined with Computational Holographic Fields

Abstract: We present a method of rendering aerial and volumetric graphics using femtosecond lasers. A high-intensity laser excites physical matter to emit light at an arbitrary three-dimensional position. Popular applications can thus be explored, especially because plasma induced by a femtosecond laser is less harmful than that generated by a nanosecond laser. There are two methods of rendering graphics with a femtosecond laser in air: producing holograms using spatial light modulation technology and scanning of a laser beam by a galvano mirror. The holograms and workspace of the system proposed here occupy a volume of up to 1 cm3; however, this size is scalable depending on the optical devices and their setup. This article provides details of the principles, system setup, and experimental evaluation, and discusses the scalability, design space, and applications of this system. We tested two laser sources: an adjustable (30--100fs) laser that projects up to 1,000 pulses/s at an energy of up to 7mJ/pulse and a 269fs laser that projects up to 200,000 pulses/s at an energy of up to 50μJ/pulse. We confirmed that the spatiotemporal resolution of volumetric displays implemented using these laser sources is 4,000 and 200,000 dots/s, respectively. Although we focus on laser-induced plasma in air, the discussion presented here is also applicable to other rendering principles such as fluorescence and microbubbles in solid or liquid materials.

Time Gating of Chloroplast Autofluorescence Allows Clearer Fluorescence Imaging In Planta.

Abstract: Chloroplast, an organelle facilitating photosynthesis, exhibits strong autofluorescence, which is an undesired background signal that restricts imaging experiments with exogenous fluorophore in plants. In this study, the autofluorescence was characterized in planta under confocal laser microscopy, and it was found that the time-gated imaging technique completely eliminates the autofluorescence. As a demonstration of the technique, a clearer signal of fluorescent protein-tagged phototropin, a blue-light photoreceptor localized at the chloroplast periphery, was visualized in planta.

RNA-seq analysis of the gonadal transcriptome during Alligator mississippiensis temperature-dependent sex determination and differentiation

Abstract: The American alligator (Alligator mississippiensis) displays temperature-dependent sex determination (TSD), in which incubation temperature during embryonic development determines the sexual fate of the individual. However, the molecular mechanisms governing this process remain a mystery, including the influence of initial environmental temperature on the comprehensive gonadal gene expression patterns occurring during TSD. Our characterization of transcriptomes during alligator TSD allowed us to identify novel candidate genes involved in TSD initiation. High-throughput RNA sequencing (RNA-seq) was performed on gonads collected from A. mississippiensis embryos incubated at both a male and a female producing temperature (33.5 °C and 30 °C, respectively) in a time series during sexual development. RNA-seq yielded 375.2 million paired-end reads, which were mapped and assembled, and used to characterize differential gene expression. Changes in the transcriptome occurring as a function of both development and sexual differentiation were extensively profiled. Forty-one differentially expressed genes were detected in response to incubation at male producing temperature, and included genes such as Wnt signaling factor WNT11, histone demethylase KDM6B, and transcription factor C/EBPA. Furthermore, comparative analysis of development- and sex-dependent differential gene expression revealed 230 candidate genes involved in alligator sex determination and differentiation, and early details of the suspected male-fate commitment were profiled. We also discovered sexually dimorphic expression of uncharacterized ncRNAs and other novel elements, such as unique expression patterns of HEMGN and ARX. Twenty-five of the differentially expressed genes identified in our analysis were putative transcriptional regulators, among which were MYBL2, MYCL, and HOXC10, in addition to conventional sex differentiation genes such as SOX9, and FOXL2. Inferred gene regulatory network was constructed, and the gene-gene and temperature-gene interactions were predicted. Gonadal global gene expression kinetics during sex determination has been extensively profiled for the first time in a TSD species. These findings provide insights into the genetic framework underlying TSD, and expand our current understanding of the developmental fate pathways during vertebrate sex determination.

Intracellular Delivery of Proteins via Fusion Peptides in Intact Plants.

Abstract: In current plant biotechnology, the introduction of exogenous DNA encoding desired traits is the most common approach used to modify plants. However, general plant transformation methods can cause random integration of exogenous DNA into the plant genome. To avoid these events, alternative methods, such as a direct protein delivery system, are needed to modify the plant. Although there have been reports of the delivery of proteins into cultured plant cells, there are currently no methods for the direct delivery of proteins into intact plants, owing to their hierarchical structures. Here, we demonstrate the efficient fusion-peptide-based delivery of proteins into intact Arabidopsis thaliana. Bovine serum albumin (BSA, 66 kDa) was selected as a model protein to optimize conditions for delivery into the cytosol. The general applicability of our method to large protein cargo was also demonstrated by the delivery of alcohol dehydrogenase (ADH, 150 kDa) into the cytosol. The compatibility of the fusion peptide system with the delivery of proteins to specific cellular organelles was also demonstrated using the fluorescent protein Citrine (27 kDa) conjugated to either a nuclear localization signal (NLS) or a peroxisomal targeting signal (PTS). In conclusion, our designed fusion peptide system can deliver proteins with a wide range of molecular weights (27 to 150 kDa) into the cells of intact A. thaliana without interfering with the organelle-targeting peptide conjugated to the protein. We expect that this efficient protein delivery system will be a powerful tool in plant biotechnology.

RNA-seq-based evaluation of bicolor tepal pigmentation in Asiatic hybrid lilies (Lilium spp.).

Abstract: Color patterns in angiosperm flowers are produced by spatially and temporally restricted deposition of pigments. Identifying the mechanisms responsible for restricted pigment deposition is a topic of broad interest. Some dicots species develop bicolor petals, which are often caused by the post-transcriptional gene silencing (PTGS) of chalcone synthase (CHS) genes. An Asiatic hybrid lily (Lilium spp.) cultivar Lollypop develops bicolor tepals with pigmented tips and white bases. Here, we analyzed the global transcription of pigmented and non-pigmented tepal parts from Lollypop, to determine the main transcriptomic differences. De novo assembly of RNA-seq data yielded 49,239 contigs (39,426 unigenes), which included a variety of novel transcripts, such as those involved in flavonoid-glycosylation and sequestration and in regulation of anthocyanin biosynthesis. Additionally, 1258 of the unigenes exhibited significantly differential expression between the tepal parts (false discovery rates 2-fold higher in the pigmented parts. Thus, LhMYB12 should be involved in the transcriptional regulation of the biosynthesis genes in bicolor tepals. Other factors that potentially suppress or enhance the expression of anthocyanin biosynthesis genes, including a WD40 gene, were identified, and their involvement in bicolor development is discussed. Our results indicate that the bicolor trait of Lollypop tepals is caused by the transcriptional regulation of anthocyanin biosynthesis genes and that the transcription profile of LhMYB12 provides a clue for elucidating the mechanisms of the trait. The tepal transcriptome constructed in this study will accelerate investigations of the genetic controls of anthocyanin color patterns, including the bicolor patterns, of Lilium spp.

Constructive spin-orbital angular momentum coupling can twist materials to create spiral structures in optical vortex illumination

Abstract: It was discovered that optical vortices twist isotropic and homogenous materials, e.g., azo-polymer films to form spiral structures on a nano- or micro-scale. However, the formation mechanism has not yet been established theoretically. To understand the mechanism of the spiral surface relief formation in the azo-polymer film, we theoretically investigate the optical radiation force induced in an isotropic and homogeneous material under irradiation using a continuous-wave optical vortex with arbitrary topological charge and polarization. It is revealed that the spiral surface relief formation in azo-polymer films requires the irradiation of optical vortices with a positive (negative) spin angular momentum and a positive (negative) orbital angular momentum (constructive spin-orbital angular momentum coupling), i.e., the degeneracy among the optical vortices with the same total angular momentum is resolved.

Dynamic terahertz spectroscopy of gas molecules mixed with unwanted aerosol under atmospheric pressure using fibre-based asynchronous-optical-sampling terahertz time-domain spectroscopy.

Abstract: Terahertz (THz) spectroscopy is a promising method for analysing polar gas molecules mixed with unwanted aerosols due to its ability to obtain spectral fingerprints of rotational transition and immunity to aerosol scattering. In this article, dynamic THz spectroscopy of acetonitrile (CH3CN) gas was performed in the presence of smoke under the atmospheric pressure using a fibre-based, asynchronous-optical-sampling THz time-domain spectrometer. To match THz spectral signatures of gas molecules at atmospheric pressure, the spectral resolution was optimized to 1 GHz with a measurement rate of 1 Hz. The spectral overlapping of closely packed absorption lines significantly boosted the detection limit to 200 ppm when considering all the spectral contributions of the numerous absorption lines from 0.2 THz to 1 THz. Temporal changes of the CH3CN gas concentration were monitored under the smoky condition at the atmospheric pressure during volatilization of CH3CN droplets and the following diffusion of the volatilized CH3CN gas without the influence of scattering or absorption by the smoke. This system will be a powerful tool for real-time monitoring of target gases in practical applications of gas analysis in the atmospheric pressure, such as combustion processes or fire accident.

Peptide Toxins in Solitary Wasp Venoms.

Abstract: Solitary wasps paralyze insects or spiders with stinging venom and feed the paralyzed preys to their larva. Accordingly, the venoms should contain a variety of constituents acting on nervous systems. However, only a few solitary wasp venoms have been chemically studied despite thousands of species inhabiting the planet. We have surveyed bioactive substances in solitary wasp venoms found in Japan and discovered a variety of novel bioactive peptides. Pompilidotoxins (PMTXs), in the venoms of the pompilid wasps Anoplius samariensis and Batozonellus maculifrons, are small peptides consisting of 13 amino acids without a disulfide bond. PMTXs slowed Na+ channel inactivation, in particular against neuronal type Na+ channels, and were rather selective to the Nav1.6 channel. Mastoparan-like cytolytic and antimicrobial peptides are the major components of eumenine wasp venoms. They are rich in hydrophobic and basic amino acids, adopting a α-helical secondary structure, and showing mast cell degranulating, antimicrobial and hemolytic activities. The venom of the spider wasp Cyphononyx fulvognathus contained four bradykinin-related peptides. They are hyperalgesic and, dependent on the structure, differently associated with B1 or B2 receptors. Further survey led to the isolation of leucomyosuppressin-like FMRFamide peptides from the venoms of the digger wasps Sphex argentatus and Isodontia harmandi. These results of peptide toxins in solitary wasp venoms from our studies are summarized.

Enhanced electrical power generation using flame-oxidized stainless steel anode in microbial fuel cells and the anodic community structure

Abstract: Carbon-based materials are commonly used as anodes in microbial fuel cells (MFCs), whereas metal and metal-oxide-based materials are not used frequently because of low electrical output. Stainless steel is a low-cost material with high conductivity and physical strength. In this study, we investigated the power generation using flame-oxidized (FO) stainless steel anodes (SSAs) in single-chambered air-cathode MFCs. The FO-SSA performance was compared to the performance of untreated SSA and carbon cloth anode (CCA), a common carbonaceous electrode. The difference in the anodic community structures was analyzed using high-throughput sequencing of the V4 region in 16S rRNA gene. Flame oxidation of SSA produced raised node-like sites, predominantly consisting of hematite (Fe2O3), on the surface, as determined by X-ray diffraction spectroscopy. The flame oxidation enhanced the maximum power density (1063 mW/m2) in MFCs, which was 184 and 24 % higher than those for untreated SSA and CCA, respectively. The FO-SSA exhibited 8.75 and 2.71 times higher current production than SSA and CCA, respectively, under potentiostatic testing conditions. Bacteria from the genus Geobacter were detected at a remarkably higher frequency in the biofilm formed on the FO-SSA (8.8–9.2 %) than in the biofilms formed on the SSA and CCA (0.7–1.4 %). Bacterial species closely related to Geobacter metallireducens (>99 % identity in the gene sequence) were predominant (93–96 %) among the genus Geobacter in the FO-SSA biofilm, whereas bacteria with a 100 % identity to G. anodireducens were abundant (>55 %) in the SSA and CCA biofilms. This is the first demonstration of power generation using an FO-SSA in MFCs. Flame oxidation of the SSA enhances electricity production in MFCs, which is higher than that with the common carbonaceous electrode, CCA. The FO-SSA is not only inexpensive but also can be prepared using a simple method. To our knowledge, this study reveals, for the first time, that the predominant Geobacter species in the biofilm depends on the anode material. The high performance of the FO-SSA could result from the particularly high population of bacteria closely related to G. metallireducens in the biofilm.

Massively parallel femtosecond laser processing.

Abstract: Massively parallel femtosecond laser processing with more than 1000 beams was demonstrated. Parallel beams were generated by a computer-generated hologram (CGH) displayed on a spatial light modulator (SLM). The key to this technique is to optimize the CGH in the laser processing system using a scheme called in-system optimization. It was analytically demonstrated that the number of beams is determined by the horizontal number of pixels in the SLM NSLM that is imaged at the pupil plane of an objective lens and a distance parameter pd obtained by dividing the distance between adjacent beams by the diffraction-limited beam diameter. A performance limitation of parallel laser processing in our system was estimated at NSLM of 250 and pd of 7.0. Based on these parameters, the maximum number of beams in a hexagonal close-packed structure was calculated to be 1189 by using an analytical equation.

Study on finishing characteristics of magnetic abrasive finishing process using low-frequency alternating magnetic field

Abstract: This paper describes a new ultra-precision magnetic abrasive finishing (MAF) process using alternating magnetic field. The process principle and the finishing characteristics are described. Specifically, the effects of finishing parameters such as cutting fluid, rotational speed of magnetic pole, and current frequency on change in material removal and surface finish are investigated respectively. Experimental results indicate that neat cutting oil is more suitable for this processing, which can obtain higher material removal and smoother finished surface compared with water-soluble cutting fluid and silicone fluid. The finishing force and material removal are gradually increasing with the increase of rotational speed of magnetic pole. It is confirmed that the angle variation of magnetic particles is decreasing with the increase of current frequency, and the few nanometer surface can be acquired in the condition of low frequency.

Neverland regulates embryonic moltings through the regulation of ecdysteroid synthesis in the water flea Daphnia magna, and may thus act as a target for chemical disruption of molting

Abstract: Embryo development in arthropods is accompanied by a series of moltings. A cladoceran crustacean Daphnia magna molts three times before reaching first instar neonate during embryogenesis. Previous studies argued ecdysteroids might regulate D. magna embryogenesis. However, no direct evidence between innate ecdysteroids fluctuation and functions has been forthcoming. Recently, we identified genes involved in ecdysteroid synthesis called, neverland (neverland1 and neverland 2) and shade and in the ecdysteroid degradation (Cyp18a1). To understand the physiological roles of ecdysteroids in D. magna embryos, we performed expression and functional analyzes of those genes. Examining innate ecdysteroids titer during embryogenesis showed two surges of ecdysteroids titer at 41 and 61 h after oviposition. The first and second embryonic moltings occurred at each ecdysteroid surge. Expression of neverland1 and shade began to increase before the first peak in ecdysteroid. Knockdown of neverland1 or shade by RNAi technique caused defects in embryonic moltings and subsequent development. The ecdysteroids titer seemingly decreased in nvd1-knowckdown embryos. Knockdown of Cyp18a1 resulted in early embryonic lethality before the first molting. Our in situ hybridization analysis revealed that nvd1 was prominently expressed in embryonic gut epithelium suggesting the site for an initial step of ecdysteroidgenesis, a conversion of cholesterol to 7-dehydrocholesterol and possibly for ecdysone production. Taken together, de novo ecdysteroid synthesis by nvd1 in the gut epithelial cells stimulates molting, which is indispensable for D. magna embryo development. These findings identify neverland as a possible target for chemicals, including various pesticides that are known to disrupt molting, development and reproduction. Copyright © 2016 John Wiley & Sons, Ltd.

Cross-Field Aerial Haptics: Rendering Haptic Feedback in Air with Light and Acoustic Fields

Abstract: We present a new method of rendering aerial haptic images that uses femtosecond-laser light fields and ultrasonic acoustic fields. In conventional research, a single physical quantity has been used to render aerial haptic images. In contrast, our method combines multiple fields (light and acoustic fields) at the same time. While these fields have no direct interference, combining them provides benefits such as multi-resolution haptic images and a synergistic effect on haptic perception. We conducted user studies with laser haptics and ultrasonic haptics separately and tested their superposition. The results showed that the acoustic field affects the tactile perception of the laser haptics. We explored augmented reality/virtual reality (AR/VR) applications such as providing haptic feedback of the combination of these two methods. We believe that the results of this study contribute to the exploration of laser haptic displays and expand the expression of aerial haptic displays based on other principles.

Cuticular protein and transcription factor genes expressed during prepupal-pupal transition and by ecdysone pulse treatment in wing discs of Bombyx mori.

Abstract: We aimed to understand the underlying mechanism that regulates successively expressed cuticular protein (CP) genes around pupation in Bombyx mori. Quantitative PCR was conducted to clarify the expression profile of CP genes and ecdysone-responsive transcription factor (ERTF) genes around pupation. Ecdysone pulse treatment was also conducted to compare the developmental profiles and the ecdysone induction of the CP and ERTF genes. Fifty-two CP genes (RR-1 13, RR-2 18, CPG 8, CPT 3, CPFL 2, CPH 8) in wing discs of B. mori were examined. Different expression profiles were found, which suggests the existence of a mechanism that regulates CP genes. We divided the genes into five groups according to their peak stages of expression. RR-2 genes were expressed until the day of pupation and RR-1 genes were expressed before and after pupation and for longer than RR-2 genes; this suggests different construction of exo- and endocuticular layers. CPG, CPT, CPFL and CPH genes were expressed before and after pupation, which implies their involvement in both cuticular layers. Expression profiles of ERTFs corresponded with previous reports. Ecdysone pulse treatment showed that the induction of CP and ERTF genes in vitro reflected developmental expression, from which we speculated that ERTFs regulate CP gene expression around pupation.

Measurement of mean thickness of transparent samples using simultaneous phase shifting interferometry with four interferograms.

Abstract: In this research a novel interferometric system is reported, which allows the generation of four simultaneous interferograms with phase shifts of π/2 The system consists of three coupled interferometers: a rectangular Sagnac interferometer which generates a primary pattern with crossed circular polarizations, coupled to two Michelson interferometers which operate as a multiplexing system, and generating replicas of the primary pattern The two coupled Michelson interferometers generate four patterns retaining their polarization properties, which allow independent phase shifts by placing a linear polarizer over each pattern, thereby, four interferograms with relative phase shifts of π/2 are obtained The optical phase is calculated using the well-known four-step algorithm With knowledge of the optical phase, different properties of the samples can be calculated or analyzed; in this case, by knowing the mean refractive index, we can calculate the mean thickness of test objects The results obtained for static transparent samples are presented The capability of the system to analyze dynamic events is shown when results for the calculation of a temperature field of a heat flow are presented

Cesium absorption through bark of Japanese cedar (Cryptomeria japonica)

Abstract: Absorption of radiocesium (137Cs and 134Cs) through bark, and its subsequent translocation into wood and needles, has been suggested as a potential source of tree contamination, but the process is not well understood. Field experiments were conducted to confirm whether Cs could enter a Japanese cedar tree through the bark and how Cs moves within a tree. Stable Cs (133Cs) was applied to the bark at 1.2-m height on 10- and 26-year-old Japanese cedars. The 133Cs concentrations were determined in the bark, sapwood, and heartwood (for 26-year-old cedar only) of stem disks from several heights, as well as in current-year needles from the canopy. The 133Cs concentrations were considerably higher in the sapwood and heartwood of stem disks from 1.2-m height in treated trees than in untreated trees, suggesting that 133Cs penetrated the bark to enter the wood. The average 133Cs concentrations were higher in the heartwood than the sapwood, indicating 133Cs accumulation in the heartwood. High 133Cs concentrations in the needles of treated trees implied acropetal movement of 133Cs to actively growing organs. Our results demonstrate that Cs can enter Japanese cedar trees through the bark and that Cs is transported radially to the heartwood and vertically to the apex.

Holographic Correloscopy—Unconventional Holographic Techniques For Imaging a Three-Dimensional Object Through an Opaque Diffuser or Via a Scattering Wall: A Review

Abstract: Techniques of unconventional holography, called holographic correloscopy, for imaging a three-dimensional (3-D) object through an opaque diffuser or via a scattering wall are reviewed. This paper is not intended to be a general overview. Instead, it will focus and shed light on less-known techniques termed coherence holography, photon-correlation holography, remote imaging digital holography, and dual-reference holography. This paper will introduce how they perform 3-D imaging through a diffuser or via a scattering wall with a thin but strongly scattering layer.

Preparation of Ru/ZrO2 Catalysts by NaBH4 Reduction and Their Catalytic Activity for NH3 Decomposition To Produce H2

Abstract: ZrO2-supported Ru catalysts were prepared by an impregnation method and by NaBH4 reduction from RuCl3·3H2O and a commercial ZrO2 support. The catalyst prepared by NaBH4 reduction showed high activity (around 60%) at 673 K for NH3 decomposition, whereas the catalyst prepared by the impregnation method exhibited low activity (10%) under the same reaction conditions. X-ray fluorescence spectrometry indicated that the catalyst prepared by NaBH4 reduction was free of Cl, whereas the catalyst prepared by the impregnation method contained more than 5 wt % Cl from the Ru precursor. This was main factor that caused the differences in catalytic activity. In addition, the Ru/ZrO2 catalyst prepared by NaBH4 reduction was active for NH3 decomposition without H2 pretreatment, and its activity was slightly higher than that of the catalyst pretreated with H2. The transmission electron microscopy and H2 pulse chemical adsorption results demonstrated that the Ru metal particle size also affected the activity.

Effects of radial growth rate on anatomical characteristics and wood properties of 10-year-old Dysoxylum mollissimum trees planted in Bengkulu, Indonesia

Abstract: The growth characteristics, anatomical characteristics, and wood properties of Dysoxylum mollissimum, a fastgrowing tree species, were investigated. To clarify the effect of radial growth rate on these characteristics and properties, 10-yearold trees planted in Bengkulu, Indonesia were examined and classified into three categories (fast-, medium-, and slow-growing). Xylem maturation type was also evaluated in this species. The mean values of stem diameter, tree height, stem volume, and stresswave velocity in the 50 measured trees were 18.0 cm, 10.6 m, 0.119 m, and 3.48 km s, respectively. Mean values of anatomical characteristics and wood properties in nine selected trees were as follows: vessel diameter, 129 μm; vessel frequency, 3.5 vessels mm; cell wall thickness of wood fiber, 1.2 μm; percentages of vessels, wood fiber, ray parenchyma, axial parenchyma, and cell wall, 8.8, 72.9, 12.4, 5.9, and 33.5%, respectively; vessel element length, 0.36 mm; wood fiber length, 1.04 mm; basic density, 0.45 g cm; and compressive strength parallel to the grain in green condition, 29.9 MPa. These obtained values were similar to or smaller than previous results obtained in research on other Dysoxylum spp. For almost all of the anatomical characteristics and wood properties, no significant differences among the categories were found. This suggests that the trees with faster radial growth characteristics do not always produce lower quality wood in this species. Judging from the radial variation of anatomical characteristics, the xylem maturation of this species depends on the diameter growth, and it might occur at around 6 to 8 cm from the pith.

Seed treatment with salicylic acid invokes defence mechanism of Helianthus annuus against Orobanche cumana

Abstract: The root holoparasitic angiosperm sunflower broomrape (Orobanche cumana) specifically affects sunflower (Helianthus annuus) growth and causes severe damage all over the world. This investigation was designed to examine the protective effects of salicylic acid (SA) treatment to the seeds of an O. cumana-susceptible cultivar of sunflower (TK0409). Sunflower seeds were pretreated with different concentrations (0, 0.5 and 1 mM) of SA and inoculated with O. cumana for 4 weeks. O. cumana infection resulted in reduction in plant biomass, endogenous SA level, and the expression of SA-related genes including pal, chs and NPR1. By contrast, O. cumana infection enhanced the production of reactive oxygen species, activities of antioxidant enzymes as well as contents of phenolics and lignin. Seed treatment with 1 mM SA increased sunflower biomass in terms of plant height, fresh weight and dry weight by 10%, 13% and 26%, respectively, via reducing the number and biomass of established O. cumana. The increase of hydrogen peroxide contents by 14% in the 1 mM SA treated sunflower plants appeared to be because of the inhibition of ascorbate peroxidase and catalase by exogenous SA. The enhanced expression of pathogenesis-related genes (PR3 and PR12, encoding chitinase and defensin, respectively) after 4 weeks of inoculation indicated that systemic acquired resistance was induced in the SA treated sunflower in which the level of endogenous SA was also elevated in a dose-dependent manner. The increased expression of a hypersensitive-responsive (HR) gene hsr indicated that the resistance of sunflowers might be associated with a hypersensitive reaction which was activated by exogenous SA treatment.

Turning the fate of reprogramming cells from retinal disorder to regeneration by Pax6 in newts.

Abstract: The newt, a urodele amphibian, has an outstanding ability– even as an adult –to regenerate a functional retina through reprogramming and proliferation of the retinal pigment epithelium (RPE) cells, even though the neural retina is completely removed from the eye by surgery. It remains unknown how the newt invented such a superior mechanism. Here we show that disability of RPE cells to regenerate the retina brings about a symptom of proliferative vitreoretinopathy (PVR), even in the newt. When Pax6, a transcription factor that is re-expressed in reprogramming RPE cells, is knocked down in transgenic juvenile newts, these cells proliferate but eventually give rise to cell aggregates that uniformly express alpha smooth muscle actin, Vimentin and N-cadherin, the markers of myofibroblasts which are a major component of the sub-/epi-retinal membranes in PVR. Our current study demonstrates that Pax6 is an essential factor that directs the fate of reprogramming RPE cells toward the retinal regeneration. The newt may have evolved the ability of retinal regeneration by modifying a mechanism that underlies the RPE-mediated retinal disorders.

Qualitative-Modeling-Based Silicon Neurons and Their Networks.

Abstract: The ionic conductance models of neuronal cells can finely reproduce a wide variety of complex neuronal activities. However, the complexity of these models has prompted the development of qualitative neuron models. They are described by differential equations with a reduced number of variables and right-hand sides expressed with low-dimensional polynomials, which retain their core mathematical structures. Such simple models form the foundation of a bottom-up approach in computational and theoretical neuroscience. We proposed a qualitative-modeling- based approach for designing silicon neuron circuits, in which the mathematical structures in the polynomial-based qualitative models are reproduced by differential equations with silicon-native expressions. This approach can realize low-power-consuming circuits that can be configured to realize various classes of neuronal cells. In this article, our qualitative-modeling-based silicon neuron circuits for analog and digital implementations are quickly reviewed. One of our CMOS analog silicon neuron circuits can realize a variety of neuronal activities with a power consumption of about 50 nW. The square-wave bursting mode of this circuit is explained. Another circuit can realize Class I and II neuronal activities with about 3 nW. Our digital silicon neuron circuit can also realize these classes. An associative memory realized on an all-to-all connected network of these silicon neurons is also reviewed, in which the neuron class plays important roles in its performance.

Topology Optimization Based on Regularized Level-Set Function for Solving 3-D Nonlinear Magnetic Field System With Spatial Symmetric Condition

Abstract: Topology optimization (TO) is an effective method for producing practical design of electrical machines. TO is conventionally implemented based on the material density; however, this approach can yield a large number of grayscale areas and unfeasible shapes. On the other hand, when the level-set function is applied to the TO of the magnetic field problem, the potential for obtaining a feasible solution improves. This paper implemented a TO method that uses the regularized level-set function to solve the 3-D nonlinear magnetic field problem. Furthermore, a spatial symmetry condition (SSC), which can introduce the mirror and periodic symmetry of the level-set function from the viewpoint of the electromagnetism, is proposed. To achieve symmetry in the electromagnetic field distribution, the validity of the SSC is investigated and confirmed in a 3-D magnetic shielding problem.