Journal ArticleDOI
Apparent sources of the A genomes of wheats inferred from polymorphism in abundance and restriction fragment length of repeated nucleotide sequences
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Four hundred random DNA fragment clones of wild diploid wheat Triticum monococcum ssp.Abstract:
Four hundred random DNA fragment clones of wild diploid wheat Triticum monococcum ssp. aegilopoides (syn. T. baeoticum) were screened for clones of repeated nucleotide sequences. Seven DNA fragment...read more
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Wild emmer genome architecture and diversity elucidate wheat evolution and domestication
Raz Avni,Moran Nave,Omer Barad,Kobi Baruch,Sven Twardziok,Heidrun Gundlach,Iago Hale,Martin Mascher,Manuel Spannagl,Krystalee Wiebe,Katherine W. Jordan,Guy Golan,Jasline Deek,Batsheva Ben-Zvi,Gil Ben-Zvi,Axel Himmelbach,Ron MacLachlan,Andrew G. Sharpe,Allan K. Fritz,Roi Ben-David,Hikmet Budak,Tzion Fahima,Abraham B. Korol,Justin D. Faris,Alvaro G. Hernandez,Mark A. Mikel,Avraham A. Levy,Brian J. Steffenson,Marco Maccaferri,Roberto Tuberosa,Luigi Cattivelli,Primetta Faccioli,Aldo Ceriotti,Khalil Kashkush,Mohammad Pourkheirandish,Takao Komatsuda,Tamar Eilam,Hanan Sela,Amir Sharon,Nir Ohad,Daniel A. Chamovitz,Klaus F. X. Mayer,Nils Stein,Gil Ronen,Zvi Peleg,Curtis J. Pozniak,Eduard Akhunov,Assaf Distelfeld +47 more
TL;DR: A 10.1-gigabase assembly of the 14 chromosomes of wild tetraploid wheat, as well as analyses of gene content, genome architecture, and genetic diversity reveal genomic regions bearing the signature of selection under domestication.
Journal ArticleDOI
Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the Triticum/Aegilops complex and the evolutionary history of polyploid wheat
Shaoxing Huang,Anchalee Sirikhachornkit,Xiujuan Su,Justin D. Faris,Bikram S. Gill,Robert Haselkorn,Piotr Gornicki +6 more
TL;DR: The data suggest that the Acc-1 and Pgk-1 loci have different histories in different lineages, indicating genome mosaicity and significant intraspecific differentiation.
Journal ArticleDOI
Durum wheat genome highlights past domestication signatures and future improvement targets
Marco Maccaferri,Marco Maccaferri,Neil S. Harris,Sven Twardziok,Raj K. Pasam,Heidrun Gundlach,Manuel Spannagl,Danara Ormanbekova,Thomas Lux,Verena M. Prade,Sara Giulia Milner,Axel Himmelbach,Martin Mascher,Paolo Bagnaresi,Primetta Faccioli,Paolo Cozzi,Massimiliano Lauria,Barbara Lazzari,Alessandra Stella,Andrea Manconi,Matteo Gnocchi,Marco Moscatelli,Raz Avni,Jasline Deek,Sezgi Biyiklioglu,Elisabetta Frascaroli,Simona Corneti,Silvio Salvi,Gabriella Sonnante,Francesca Desiderio,Caterina Marè,Cristina Crosatti,E. Mica,Hakan Özkan,Benjamin Kilian,Pasquale De Vita,Daniela Marone,Reem Joukhadar,Elisabetta Mazzucotelli,Domenica Nigro,Agata Gadaleta,Shiaoman Chao,Justin D. Faris,Arthur T. O. Melo,Michael O. Pumphrey,Nicola Pecchioni,Luciano Milanesi,Krystalee Wiebe,Jennifer Ens,Ron MacLachlan,John M. Clarke,Andrew G. Sharpe,Chu Shin Koh,Kevin Y. H. Liang,Gregory J. Taylor,Ron Knox,Hikmet Budak,Anna M. Mastrangelo,Steven S. Xu,Nils Stein,Iago Hale,Assaf Distelfeld,Matthew J. Hayden,Roberto Tuberosa,Sean Walkowiak,Klaus F. X. Mayer,Aldo Ceriotti,Curtis J. Pozniak,Luigi Cattivelli +68 more
TL;DR: The assembly of the genome of durum wheat cultivar Svevo enables genome-wide genetic diversity analyses highlighting modifications imposed by thousands of years of empirical selection and breeding.
Journal ArticleDOI
Uncovering hidden variation in polyploid wheat.
Ksenia V. Krasileva,Ksenia V. Krasileva,Ksenia V. Krasileva,Hans A. Vasquez-Gross,Tyson Howell,Paul Bailey,Francine Paraiso,Leah Clissold,James Simmonds,Ricardo H. Ramirez-Gonzalez,Xiaodong Wang,Philippa Borrill,Christine Fosker,Sarah Ayling,Andrew L. Phillips,Cristobal Uauy,Jorge Dubcovsky,Jorge Dubcovsky +17 more
TL;DR: The protein coding regions of 2,735 mutant lines of tetraploid and hexaploid wheat were sequenced and a public database including more than 10 million mutations was developed, enabling rapid identification of mutations in the different copies of the wheat genes.
Journal ArticleDOI
Multiple wheat genomes reveal global variation in modern breeding.
Sean Walkowiak,Sean Walkowiak,Liangliang Gao,Cécile Monat,Georg Haberer,Mulualem T. Kassa,Jemima Brinton,Ricardo H. Ramirez-Gonzalez,Markus C. Kolodziej,Emily Delorean,Dinushika Thambugala,Valentyna Klymiuk,Brook Byrns,Heidrun Gundlach,Venkat Bandi,Jorge Nunez Siri,Kirby T. Nilsen,Catharine Aquino,Axel Himmelbach,Dario Copetti,Dario Copetti,Tomohiro Ban,Luca Venturini,Michael W. Bevan,Bernardo J. Clavijo,Dal-Hoe Koo,Jennifer Ens,Krystalee Wiebe,Amidou N’Diaye,Allen K. Fritz,Carl Gutwin,Anne Fiebig,Christine Fosker,Bin Xiao Fu,Gonzalo Garcia Accinelli,Keith A. Gardner,Nick Fradgley,Juan J. Gutierrez-Gonzalez,Gwyneth Halstead-Nussloch,Masaomi Hatakeyama,Chu Shin Koh,Jasline Deek,Alejandro C. Costamagna,Pierre R. Fobert,Darren Heavens,Hiroyuki Kanamori,Kanako Kawaura,Fuminori Kobayashi,Ksenia V. Krasileva,Tony Kuo,Tony Kuo,Neil McKenzie,Kazuki Murata,Yusuke Nabeka,Timothy Paape,Sudharsan Padmarasu,Lawrence Percival-Alwyn,Sateesh Kagale,Uwe Scholz,Jun Sese,Philomin Juliana,Ravi P. Singh,Rie Shimizu-Inatsugi,David Swarbreck,James Cockram,Hikmet Budak,Toshiaki Tameshige,Tsuyoshi Tanaka,Hiroyuki Tsuji,Jonathan M. Wright,Jianzhong Wu,Burkhard Steuernagel,Ian Small,Sylvie Cloutier,Gabriel Keeble-Gagnère,Gary J. Muehlbauer,Josquin Tibbets,Shuhei Nasuda,Joanna Melonek,Pierre Hucl,Andrew G. Sharpe,Matthew D. Clark,Erik Legg,Arvind K. Bharti,Peter Langridge,Anthony Hall,Cristobal Uauy,Martin Mascher,Simon G. Krattinger,Simon G. Krattinger,Hirokazu Handa,Kentaro Shimizu,Kentaro Shimizu,Assaf Distelfeld,Kenneth J. Chalmers,Beat Keller,Klaus F. X. Mayer,Jesse Poland,Nils Stein,Nils Stein,Curt A. McCartney,Manuel Spannagl,Thomas Wicker,Curtis J. Pozniak +103 more
TL;DR: Comparative analysis of multiple genome assemblies from wheat reveals extensive diversity that results from the complex breeding history of wheat and provides a basis for further potential improvements to this important food crop.
References
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Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Jeffrey Vieira,Joachim Messing +1 more
TL;DR: A series of plasmid vectors containing the multiple cloning site (MCS7) of M13mp7 has been constructed and a kanamycin-resistance marker has been inserted into the center of the symmetrical MCS7 to yield a restriction-site-mobilizing element (RSM).
Journal ArticleDOI
Dideoxy sequencing method using denatured plasmid templates.
TL;DR: The dideoxy sequencing method in which denatured plasmid DNA is used as a template was improved and enabled us to determine the sequence of more than a thousand nucleotides from a single template DNA.
Journal ArticleDOI
Morphological evidence concerning the origin of the b genome in wheat
TL;DR: In order to discover an unknown parent involved in the phylogeny of an allopolyploid, the method of genome analysis can be used only by a process of trial and error, and chromosome pairing in the hybrids between tetraploid wheat and its supposed diploid relatives has not clarified the relationship of the B genome.
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