Journal ArticleDOI
Biophysical regulation of epigenetic state and cell reprogramming
Timothy L. Downing,Jennifer Soto,Constant Morez,Constant Morez,Timothee Houssin,Timothee Houssin,Ashley L. Fritz,Falei Yuan,Falei Yuan,Julia Chu,Shyam Patel,David V. Schaffer,Song Li +12 more
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TLDR
It is shown that biophysical cues, in the form of parallel microgrooves on the surface of cell-adhesive substrates, can replace the effects of small-molecule epigenetic modifiers and significantly improve reprogramming efficiency and promote a mesenchymal-to-epithelial transition in adult fibroblasts.Abstract:
Somatic cells can be reprogrammed into induced pluripotent stem cells biochemically through the expression of a few transcription factors. It is now shown that aligned microgrooves or nanofibres on cell-adhesive substrates can promote the reprogramming of somatic cells more efficiently through epigenetic regulation of genes related to pluripotency and the mesenchymal-to-epithelial transition. The findings suggest that the epigenetic state can be regulated by variations in cell morphology.read more
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Lateral confined growth of cells activates Lef1 dependent pathways to regulate cell-state transitions
TL;DR: In this article , the authors identify Lef1 as a critical somatic transcription factor for the mechanical regulation of de-differentiation pathways and show that knockdown results in down-regulation of fibroblast dedifferentiation.
Microfabricated systems integrated with biomolecular probes for cell mechanics
TL;DR: In this paper, the authors proposed a method to solve the problem of the problem: the one-dimensional graph.By.By, by.by, by, and by.
Journal ArticleDOI
Nanoengineered materials to control cell fate.
TL;DR: Surfaces can be nanoengineered in order to affect adhesion plaque size, orientation and shape, thus affecting the mechanical identity of the cell through its cytoskeletal assembly, and the capability of manipulating material properties at the nanoscale is key central.
Journal ArticleDOI
A Multisensory Network Drives Nuclear Mechanoadaptation
TL;DR: The nucleus has the intrinsic structural components needed to receive and interpret mechanical inputs, but also rely on extra nuclear mechano-sensors that activate nuclear regulators in response to force, which ensures that the nucleus has a tunable response to mechanical cues.
Journal ArticleDOI
Mechanotransduction Regulates Reprogramming Enhancement in Adherent 3D Keratocyte Cultures
Shenyang Li,Chengcheng Ding,Yonglong Guo,Yanan Zhang,Hao Wang,Xihao Sun,Jun Zhang,Zekai Cui,Jiansu Chen,Jiansu Chen +9 more
TL;DR: Polydimethylsiloxane (PDMS), with hydrophobic and soft substrate properties, facilitates adherent spheroid formation that promotes cellular physical reprogramming into stem-like cells without using transcription factors as mentioned in this paper.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Supporting Online Material for Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron M. Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: Yu et al. as discussed by the authors proposed online material for induced pluripotent stem cell lines derived from human Somatic Cells, which can be used for transplanting human stem cells to humans.