Journal ArticleDOI
Biophysical regulation of epigenetic state and cell reprogramming
Timothy L. Downing,Jennifer Soto,Constant Morez,Constant Morez,Timothee Houssin,Timothee Houssin,Ashley L. Fritz,Falei Yuan,Falei Yuan,Julia Chu,Shyam Patel,David V. Schaffer,Song Li +12 more
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TLDR
It is shown that biophysical cues, in the form of parallel microgrooves on the surface of cell-adhesive substrates, can replace the effects of small-molecule epigenetic modifiers and significantly improve reprogramming efficiency and promote a mesenchymal-to-epithelial transition in adult fibroblasts.Abstract:
Somatic cells can be reprogrammed into induced pluripotent stem cells biochemically through the expression of a few transcription factors. It is now shown that aligned microgrooves or nanofibres on cell-adhesive substrates can promote the reprogramming of somatic cells more efficiently through epigenetic regulation of genes related to pluripotency and the mesenchymal-to-epithelial transition. The findings suggest that the epigenetic state can be regulated by variations in cell morphology.read more
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Mechanoresponsive stem cells to target cancer metastases through biophysical cues
Linan Liu,Shirley X. Zhang,Wenbin Liao,Henry P. Farhoodi,Chi Wut Wong,Claire C. Chen,Aude I. Segaliny,Jenu V. Chacko,Lily Nguyen,Mengrou Lu,George Polovin,Egest J. Pone,Timothy L. Downing,Devon A. Lawson,Michelle A. Digman,Michelle A. Digman,Weian Zhao +16 more
TL;DR: The proposed mechanoresponsive cell system (MRCS) indicates that biophysical cues, specifically matrix stiffness, are appealing targets for cancer treatment due to their long persistence in the body, making them refractory to the development of resistance to treatment.
Journal ArticleDOI
Pore orientation mediated control of mechanical behavior of scaffolds and its application in cartilage-mimetic scaffold design
TL;DR: It is established that mechanical behavior of porous scaffolds can be modulated by varying pore orientation alone and paved the way to recreate the structural and consequent mechanical anisotropy of articular cartilage tissue using zonally varied pore Orientation in scaffolds.
Journal ArticleDOI
The effect of exposure to nanoparticles and nanomaterials on the mammalian epigenome
TL;DR: The challenge for the future will be to determine the molecular pathways driving these epigenetic alterations, the possible functional consequences, and the potential effects on health.
Journal ArticleDOI
An epigenetic bioactive composite scaffold with well-aligned nanofibers for functional tendon tissue engineering.
Can Zhang,Xianliu Wang,Erchen Zhang,Long Yang,Huihua Yuan,Wenjing Tu,Huilan Zhang,Zi Yin,Weiliang Shen,Xiao Chen,Yanzhong Zhang,Hongwei Ouyang +11 more
TL;DR: It is demonstrated that HDAC was involved in the teno-differentiation with aligned fiber topography, and the combination of HDAC with aligned topography might be a more efficient strategy to promote tenogenesis of stem cells.
Journal ArticleDOI
Chemical reprogramming and transdifferentiation.
TL;DR: The recent achievements of chemical reprogramming/transdifferentiation are reviewed and the advantages and challenges facing this strategy in future applications are discussed.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Supporting Online Material for Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron M. Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: Yu et al. as discussed by the authors proposed online material for induced pluripotent stem cell lines derived from human Somatic Cells, which can be used for transplanting human stem cells to humans.