Journal ArticleDOI
Biophysical regulation of epigenetic state and cell reprogramming
Timothy L. Downing,Jennifer Soto,Constant Morez,Constant Morez,Timothee Houssin,Timothee Houssin,Ashley L. Fritz,Falei Yuan,Falei Yuan,Julia Chu,Shyam Patel,David V. Schaffer,Song Li +12 more
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TLDR
It is shown that biophysical cues, in the form of parallel microgrooves on the surface of cell-adhesive substrates, can replace the effects of small-molecule epigenetic modifiers and significantly improve reprogramming efficiency and promote a mesenchymal-to-epithelial transition in adult fibroblasts.Abstract:
Somatic cells can be reprogrammed into induced pluripotent stem cells biochemically through the expression of a few transcription factors. It is now shown that aligned microgrooves or nanofibres on cell-adhesive substrates can promote the reprogramming of somatic cells more efficiently through epigenetic regulation of genes related to pluripotency and the mesenchymal-to-epithelial transition. The findings suggest that the epigenetic state can be regulated by variations in cell morphology.read more
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Molecular Regulators of Cellular Mechanoadaptation at Cell-Material Interfaces
TL;DR: Understanding cellular responses to various extracellular forces is expected to provide new insights into how cellular mechanoadaptation is modulated by manipulating the mechanics of extrace cellular matrix and the application of these materials in clinical aspects.
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Thermally Stable Honeycomb‐Patterned Porous Films of a Poly(L‐lactic acid) and Poly(D‐lactic acid) Stereo Complex Prepared Using the Breath Figure Technique
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ParaStamp and Its Applications to Cell Patterning, Drug Synergy Screening, and Rewritable Devices for Droplet Storage
TL;DR: The ParaStamp technique is revealed to possess applications for cell patterning, drug screening, and rewritable functional patterning and to meet the high‐throughput requirement for industries.
Journal ArticleDOI
Is Human-induced Pluripotent Stem Cell the Best Optimal?
TL;DR: The recent availability of human cardiomyocytes derived from iPSCs opens new opportunities to build in vitro models of cardiac disease, screening for new drugs and patient-specific cardiac therapy, and several important issues remain to be addressed.
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Genetically Engineered Plant Viral Nanoparticles Direct Neural Cells Differentiation and Orientation.
TL;DR: The genetically modified viruses assembled with aligned orientation in the capillary were able to dictate directional neurite outgrowth of N2a cells, and could be potentially applied for neural tissue engineering, as a neural conduit for repairing peripheral nerve injuries.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
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Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Supporting Online Material for Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron M. Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: Yu et al. as discussed by the authors proposed online material for induced pluripotent stem cell lines derived from human Somatic Cells, which can be used for transplanting human stem cells to humans.