Journal ArticleDOI
Biophysical regulation of epigenetic state and cell reprogramming
Timothy L. Downing,Jennifer Soto,Constant Morez,Constant Morez,Timothee Houssin,Timothee Houssin,Ashley L. Fritz,Falei Yuan,Falei Yuan,Julia Chu,Shyam Patel,David V. Schaffer,Song Li +12 more
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TLDR
It is shown that biophysical cues, in the form of parallel microgrooves on the surface of cell-adhesive substrates, can replace the effects of small-molecule epigenetic modifiers and significantly improve reprogramming efficiency and promote a mesenchymal-to-epithelial transition in adult fibroblasts.Abstract:
Somatic cells can be reprogrammed into induced pluripotent stem cells biochemically through the expression of a few transcription factors. It is now shown that aligned microgrooves or nanofibres on cell-adhesive substrates can promote the reprogramming of somatic cells more efficiently through epigenetic regulation of genes related to pluripotency and the mesenchymal-to-epithelial transition. The findings suggest that the epigenetic state can be regulated by variations in cell morphology.read more
Citations
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RNA-seq reveals diverse effects of substrate stiffness on mesenchymal stem cells.
TL;DR: It is found that stiffness can regulate the expression of MSC immunomodulatory markers in response to cytokine stimulation, and the value of coupling -omics approaches with biophysical experiments is demonstrated.
Journal ArticleDOI
Mechanotransduction via the LINC complex regulates DNA replication in myonuclei.
Shuoshuo Wang,Elizabeth Stoops,Unnikannan Cp,Barak Markus,Adriana Reuveny,Elly Ordan,Talila Volk +6 more
TL;DR: De deleting components of the linker of nucleoskeleton and cytoskeleton (LINC) complex in Drosophila melanogaster larval muscles abolishes the controlled and synchronized DNA endoreplication, typical of nuclei across my ofibers, resulting in increased and variable DNA content in myonuclei of individual myofibers.
Journal ArticleDOI
Laterally confined growth of cells induces nuclear reprogramming in the absence of exogenous biochemical factors.
Bibhas Roy,Saradha Venkatachalapathy,Prasuna Ratna,Yejun Wang,Doorgesh Sharma Jokhun,Mallika Nagarajan,G. V. Shivashankar +6 more
TL;DR: It is shown that laterally confined growth of fibroblasts on micropatterned substrates induces nuclear reprogramming with high efficiency in the absence of any exogenous reprograming factors, and the induction of cancer stemness properties in MCF7 cells grown in such laterals confined conditions.
Journal ArticleDOI
Mechanical Regulation of Transcription: Recent Advances.
Kaustubh Wagh,Kaustubh Wagh,Momoko Ishikawa,David A. Garcia,David A. Garcia,Diana A. Stavreva,Arpita Upadhyaya,Gordon L. Hager +7 more
TL;DR: In this paper, the authors highlight the importance of mechanotransduction as one of the governing principles of cancer progression and emphasize the need to conduct further studies of the molecular mechanisms involved in sensing mechanical cues and coordinating transcriptional responses.
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Nanofiber-mediated microRNA delivery to enhance differentiation and maturation of oligodendroglial precursor cells
TL;DR: A nanofiber-mediated microRNA (miR) delivery method to control oligodendrocyte differentiation and myelination through a combination of fiber topography and gene silencing is developed and demonstrates the efficacy of nanofibers in providing topographical cues and microRNA reverse transfection to direct OPC differentiation.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Supporting Online Material for Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron M. Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: Yu et al. as discussed by the authors proposed online material for induced pluripotent stem cell lines derived from human Somatic Cells, which can be used for transplanting human stem cells to humans.