Journal ArticleDOI
Biophysical regulation of epigenetic state and cell reprogramming
Timothy L. Downing,Jennifer Soto,Constant Morez,Constant Morez,Timothee Houssin,Timothee Houssin,Ashley L. Fritz,Falei Yuan,Falei Yuan,Julia Chu,Shyam Patel,David V. Schaffer,Song Li +12 more
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TLDR
It is shown that biophysical cues, in the form of parallel microgrooves on the surface of cell-adhesive substrates, can replace the effects of small-molecule epigenetic modifiers and significantly improve reprogramming efficiency and promote a mesenchymal-to-epithelial transition in adult fibroblasts.Abstract:
Somatic cells can be reprogrammed into induced pluripotent stem cells biochemically through the expression of a few transcription factors. It is now shown that aligned microgrooves or nanofibres on cell-adhesive substrates can promote the reprogramming of somatic cells more efficiently through epigenetic regulation of genes related to pluripotency and the mesenchymal-to-epithelial transition. The findings suggest that the epigenetic state can be regulated by variations in cell morphology.read more
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A Src-H3 acetylation signaling axis integrates macrophage mechanosensation with inflammatory response.
Praveen Krishna Veerasubramanian,Hanjuan Shao,Vijaykumar S. Meli,Tri Andrew Q. Phan,Thuy U. Luu,Wendy Liu,Timothy L. Downing +6 more
TL;DR: In this article, the authors reveal a cytoskeleton-dependent Src-H3 acetylation (H3Ac) axis responsible for inflammation-associated histone hyperacetylation.
Journal ArticleDOI
Micropillar-based culture platform induces epithelial-mesenchymal transition in the alveolar epithelial cell line.
TL;DR: A previously undefined role of mechanical microenvironment in EMT induction is reported, and preliminarily the role of PI3K/Akt signaling pathway in mechanical micro environment regulation of EMT is investigated.
Journal ArticleDOI
Microtopography Attenuates Endothelial Cell Proliferation by Regulating MicroRNAs
TL;DR: Insight is provided into the modulation of EC functions by microtopographic cues, and the rational design of microstructured materials for cell and tissue engineering is facilitated, suggested that microgrooved surface may regulate microRNA levels and thus EC functions.
Journal ArticleDOI
Targeting Epigenetic Dependencies in Solid Tumors: Evolutionary Landscape Beyond Germ Layers Origin
Francesca Citron,Linda Fabris +1 more
TL;DR: The pivotal role of chromatin remodeling in shaping the tumor architecture and modulating tumor fitness in a microenvironment-dependent context is discussed and recent advances in the epigenome targeting are presented.
Journal ArticleDOI
Early time-point cell morphology classifiers successfully predict human bone marrow stromal cell differentiation modulated by fiber density in nanofiber scaffolds.
TL;DR: In this paper, the predictive power of day 1 cell morphology, quantified by a machine learning based method, as an indicator of osteogenic differentiation modulated by nanofiber density was investigated.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Supporting Online Material for Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron M. Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: Yu et al. as discussed by the authors proposed online material for induced pluripotent stem cell lines derived from human Somatic Cells, which can be used for transplanting human stem cells to humans.