Journal ArticleDOI
Biophysical regulation of epigenetic state and cell reprogramming
Timothy L. Downing,Jennifer Soto,Constant Morez,Constant Morez,Timothee Houssin,Timothee Houssin,Ashley L. Fritz,Falei Yuan,Falei Yuan,Julia Chu,Shyam Patel,David V. Schaffer,Song Li +12 more
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TLDR
It is shown that biophysical cues, in the form of parallel microgrooves on the surface of cell-adhesive substrates, can replace the effects of small-molecule epigenetic modifiers and significantly improve reprogramming efficiency and promote a mesenchymal-to-epithelial transition in adult fibroblasts.Abstract:
Somatic cells can be reprogrammed into induced pluripotent stem cells biochemically through the expression of a few transcription factors. It is now shown that aligned microgrooves or nanofibres on cell-adhesive substrates can promote the reprogramming of somatic cells more efficiently through epigenetic regulation of genes related to pluripotency and the mesenchymal-to-epithelial transition. The findings suggest that the epigenetic state can be regulated by variations in cell morphology.read more
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A Blueprint for Translational Regenerative Medicine
James P. K. Armstrong,Timothy J. Keane,Anne C. Roques,P. Stephen Patrick,Claire M. Mooney,Wei-Li Kuan,Venkat Pisupati,Richard O.C. Oreffo,Daniel J. Stuckey,Fiona M. Watt,Stuart J. Forbes,Roger A. Barker,Molly M. Stevens +12 more
TL;DR: In this article, the authors present a proposed blueprint for translational regenerative medicine, which offers principles to help guide the selection of cells and materials, present key in vivo imaging modalities, and argue that the host immune response should be considered throughout design and development.
Journal ArticleDOI
Immunogold FIB‐SEM: Combining Volumetric Ultrastructure Visualization with 3D Biomolecular Analysis to Dissect Cell–Environment Interactions
Sahana Gopal,Ciro Chiappini,Ciro Chiappini,James P. K. Armstrong,Qu Chen,Andrea Serio,Andrea Serio,Chia-Chen Hsu,Christoph Meinert,Travis J. Klein,Dietmar W. Hutmacher,Stephen Rothery,Molly M. Stevens +12 more
TL;DR: Investigation of the localization of histone epigenetic modifications in neural stem cells cultured on microstructured substrates and the distribution of nuclear pore complexes in myoblasts differentiated on a soft hydrogel surface using immunogold FIB‐SEM shows the potential for broad applicability to correlate structure and function with nanoscale resolution when addressing questions across cell biology, biomaterials, and regenerative medicine.
Journal ArticleDOI
Egg-in-cube: design and fabrication of a novel artificial eggshell with functionalized surface
TL;DR: A novel artificial eggshell with functionalized surface is proposed, which allows the total amount of oxygen to pass into the egg for the chick embryo culturing and has high observability and accessibility for embryo manipulation.
Journal ArticleDOI
Grid-like surface structures in thermoplastic polyurethane induce anti-inflammatory and anti-fibrotic processes in bone marrow-derived mesenchymal stem cells
Yvonne Roger,Luisa Marilena Schäck,Anastasia Koroleva,Sandra Noack,Kestutis Kurselis,Christian Krettek,Boris N. Chichkov,Thomas Lenarz,Athanasia Warnecke,Andrea Hoffmann +9 more
TL;DR: In this article, a grid-like square cavities were generated on thermoplastic polyurethane (TPU) surfaces using a combination of femtosecond laser ablation and replication methods, which were tested as potential scaffolds for human bone marrow-derived mesenchymal stem cells (MSCs) in order to use it on neural prostheses.
Journal ArticleDOI
Reprogramming Enhancers in Somatic Cell Nuclear Transfer, iPSC Technology, and Direct Conversion.
TL;DR: This review lists the majorReprogramming enhancers verified in the representative reprogramming technologies of somatic cell nuclear transfer, induced pluripotent stem cell technology, and direct conversion, and categorizes them according to their functions.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Supporting Online Material for Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron M. Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: Yu et al. as discussed by the authors proposed online material for induced pluripotent stem cell lines derived from human Somatic Cells, which can be used for transplanting human stem cells to humans.