Journal ArticleDOI
Biophysical regulation of epigenetic state and cell reprogramming
Timothy L. Downing,Jennifer Soto,Constant Morez,Constant Morez,Timothee Houssin,Timothee Houssin,Ashley L. Fritz,Falei Yuan,Falei Yuan,Julia Chu,Shyam Patel,David V. Schaffer,Song Li +12 more
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TLDR
It is shown that biophysical cues, in the form of parallel microgrooves on the surface of cell-adhesive substrates, can replace the effects of small-molecule epigenetic modifiers and significantly improve reprogramming efficiency and promote a mesenchymal-to-epithelial transition in adult fibroblasts.Abstract:
Somatic cells can be reprogrammed into induced pluripotent stem cells biochemically through the expression of a few transcription factors. It is now shown that aligned microgrooves or nanofibres on cell-adhesive substrates can promote the reprogramming of somatic cells more efficiently through epigenetic regulation of genes related to pluripotency and the mesenchymal-to-epithelial transition. The findings suggest that the epigenetic state can be regulated by variations in cell morphology.read more
Citations
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Electrospun biomaterial scaffolds with varied topographies for neuronal differentiation of human-induced pluripotent stem cells.
Nima Khadem Mohtaram,Junghyuk Ko,Craig King,Lin Sun,Nathan J. Muller,Martin B.G. Jun,Stephanie M. Willerth +6 more
TL;DR: The novel bimodal scaffolds supported the neuronal differentiation of human iPSCs as they presented both physical and chemical cues to these cells, encouraging their differentiation.
Journal ArticleDOI
Nanotechnology-Based Approaches for Guiding Neural Regeneration
TL;DR: The nanotechnology-based approaches this group has recently developed to guide stem-cell-based neural regeneration offer the precise physicochemical control required to generate tools suitable for applications in neuroscience.
Journal ArticleDOI
The biophysical, biochemical, and biological toolbox for tenogenic phenotype maintenance in vitro
TL;DR: Recreating an artificial in vivo tendon niche by engineering functional in vitro microenvironments is a research priority and Clinically relevant cell based therapies for tendon repair and regeneration could be created using tools that harness biophysical beacons, biochemical cues, and biological signals.
Journal ArticleDOI
Modulating the Substrate Stiffness to Manipulate Differentiation of Resident Liver Stem Cells and to Improve the Differentiation State of Hepatocytes
Angela Maria Cozzolino,Valeria Noce,Cecilia Battistelli,Alessandra Marchetti,Germana Grassi,Carla Cicchini,Marco Tripodi,Laura Amicone +7 more
TL;DR: Cell lines as in vitro models of liver stem cells and hepatocytes and an innovative culture method that takes into account the substrate stiffness to obtain a rapid and efficient differentiation process and the maintenance of the fully differentiated phenotype are proposed.
Journal ArticleDOI
Matrix elasticity, replicative senescence and DNA methylation patterns of mesenchymal stem cells.
Anne Schellenberg,Sylvia Joussen,Kristin Moser,Kristin Moser,Nico Hampe,Nils Hersch,Hatim Hemeda,Jan Schnitker,Bernd Denecke,Qiong Lin,Norbert Pallua,Martin Zenke,Rudolf Merkel,Bernd Hoffmann,Wolfgang Wagner +14 more
TL;DR: The results support the notion that matrix elasticity influences cellular behavior while the cells reside on the substrate, but it does not have major impact on cell-intrinsic lineage determination, replicative senescence or DNAm patterns.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Supporting Online Material for Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron M. Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: Yu et al. as discussed by the authors proposed online material for induced pluripotent stem cell lines derived from human Somatic Cells, which can be used for transplanting human stem cells to humans.