Journal ArticleDOI
Biophysical regulation of epigenetic state and cell reprogramming
Timothy L. Downing,Jennifer Soto,Constant Morez,Constant Morez,Timothee Houssin,Timothee Houssin,Ashley L. Fritz,Falei Yuan,Falei Yuan,Julia Chu,Shyam Patel,David V. Schaffer,Song Li +12 more
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TLDR
It is shown that biophysical cues, in the form of parallel microgrooves on the surface of cell-adhesive substrates, can replace the effects of small-molecule epigenetic modifiers and significantly improve reprogramming efficiency and promote a mesenchymal-to-epithelial transition in adult fibroblasts.Abstract:
Somatic cells can be reprogrammed into induced pluripotent stem cells biochemically through the expression of a few transcription factors. It is now shown that aligned microgrooves or nanofibres on cell-adhesive substrates can promote the reprogramming of somatic cells more efficiently through epigenetic regulation of genes related to pluripotency and the mesenchymal-to-epithelial transition. The findings suggest that the epigenetic state can be regulated by variations in cell morphology.read more
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Harnessing nanotopography and integrin–matrix interactions to influence stem cell fate
TL;DR: How cell adhesions interact with nanotopography is discussed, and insight is provided as to how materials scientists can exploit these interactions to direct stem cell fate and to understand how the behaviour of stem cells in their niche can be controlled.
Journal ArticleDOI
Materials as stem cell regulators
TL;DR: Recent evidence that shows that inherent material properties may be engineered to dictate stem cell fate decisions are discussed, and a subset of the operative signal transduction mechanisms that have begun to emerge are overviewed.
Journal ArticleDOI
Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications
Anna Gilpin,Yong Yang +1 more
TL;DR: The focus of this review is to compare the advantages and disadvantages of these methods in terms of their ability to retain desired ECM characteristics for particular tissues and organs.
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Engineered biomaterials for in situ tissue regeneration
TL;DR: Two biomaterials approaches to control the regenerative capacity of the body for tissue-specific regeneration by modulating the extracellular microenvironment or driving cellular reprogramming are outlined.
Journal ArticleDOI
Bioengineering strategies to accelerate stem cell therapeutics
TL;DR: Stem cells hold tremendous regenerative potential, and several exciting clinical applications are on the horizon, so bioengineering technologies are poised to overcome current bottlenecks and revolutionize the field of regenerative medicine.
References
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Journal ArticleDOI
Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
Journal ArticleDOI
Reprogramming of human somatic cells to pluripotency with defined factors
In-Hyun Park,In-Hyun Park,Rui Zhao,Rui Zhao,Jason A. West,Jason A. West,Akiko Yabuuchi,Akiko Yabuuchi,Hongguang Huo,Hongguang Huo,Tan A. Ince,Paul H. Lerou,M. William Lensch,M. William Lensch,George Q. Daley,George Q. Daley +15 more
TL;DR: The data demonstrate that defined factors can reprogramme human cells to pluripotency, and establish a method whereby patient-specific cells might be established in culture.
Journal ArticleDOI
In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
Marius Wernig,Alexander Meissner,Ruth K. Foreman,Tobias Brambrink,Manching Ku,Konrad Hochedlinger,Konrad Hochedlinger,Bradley E. Bernstein,Bradley E. Bernstein,Rudolf Jaenisch +9 more
TL;DR: The results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells.
Journal ArticleDOI
Growth Factors, Matrices, and Forces Combine and Control Stem Cells
TL;DR: Multifaceted technologies are increasingly required to produce and interrogate cells ex vivo, to build predictive models, and, ultimately, to enhance stem cell integration in vivo for therapeutic benefit.
Journal ArticleDOI
Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes by Defined Factors
Masaki Ieda,Ji-Dong Fu,Paul Delgado-Olguin,Vasanth Vedantham,Yohei Hayashi,Benoit G. Bruneau,Deepak Srivastava +6 more
TL;DR: It is believed that functional cardiomyocytes can be directly reprogrammed from differentiated somatic cells by defined factors, and the reprogramming of endogenous or explanted fibroblasts might provide a source of cardiomeocytes for regenerative approaches.