Journal ArticleDOI
Biophysical regulation of epigenetic state and cell reprogramming
Timothy L. Downing,Jennifer Soto,Constant Morez,Constant Morez,Timothee Houssin,Timothee Houssin,Ashley L. Fritz,Falei Yuan,Falei Yuan,Julia Chu,Shyam Patel,David V. Schaffer,Song Li +12 more
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TLDR
It is shown that biophysical cues, in the form of parallel microgrooves on the surface of cell-adhesive substrates, can replace the effects of small-molecule epigenetic modifiers and significantly improve reprogramming efficiency and promote a mesenchymal-to-epithelial transition in adult fibroblasts.Abstract:
Somatic cells can be reprogrammed into induced pluripotent stem cells biochemically through the expression of a few transcription factors. It is now shown that aligned microgrooves or nanofibres on cell-adhesive substrates can promote the reprogramming of somatic cells more efficiently through epigenetic regulation of genes related to pluripotency and the mesenchymal-to-epithelial transition. The findings suggest that the epigenetic state can be regulated by variations in cell morphology.read more
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Harnessing nanotopography and integrin–matrix interactions to influence stem cell fate
TL;DR: How cell adhesions interact with nanotopography is discussed, and insight is provided as to how materials scientists can exploit these interactions to direct stem cell fate and to understand how the behaviour of stem cells in their niche can be controlled.
Journal ArticleDOI
Materials as stem cell regulators
TL;DR: Recent evidence that shows that inherent material properties may be engineered to dictate stem cell fate decisions are discussed, and a subset of the operative signal transduction mechanisms that have begun to emerge are overviewed.
Journal ArticleDOI
Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications
Anna Gilpin,Yong Yang +1 more
TL;DR: The focus of this review is to compare the advantages and disadvantages of these methods in terms of their ability to retain desired ECM characteristics for particular tissues and organs.
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Engineered biomaterials for in situ tissue regeneration
TL;DR: Two biomaterials approaches to control the regenerative capacity of the body for tissue-specific regeneration by modulating the extracellular microenvironment or driving cellular reprogramming are outlined.
Journal ArticleDOI
Bioengineering strategies to accelerate stem cell therapeutics
TL;DR: Stem cells hold tremendous regenerative potential, and several exciting clinical applications are on the horizon, so bioengineering technologies are poised to overcome current bottlenecks and revolutionize the field of regenerative medicine.
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Journal ArticleDOI
Current methods for inducing pluripotency in somatic cells
TL;DR: An overview on current reprogramming protocols is provided, discusses their pros and cons and future challenges to provide safe and transgene‐free iPSCs.