Cell stiffness is a biomarker of the metastatic potential of ovarian cancer cells.
Wenwei Xu,Roman Mezencev,Byungkyu Kim,Lijuan Wang,John F. McDonald,Todd Sulchek,Todd Sulchek +6 more
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TLDR
In this paper, a detailed examination of highly invasive ovarian cancer cells relative to their less invasive parental cells (HEY) demonstrates that deformability is also an accurate biomarker of metastatic potential.Abstract:
The metastatic potential of cells is an important parameter in the design of optimal strategies for the personalized treatment of cancer. Using atomic force microscopy (AFM), we show, consistent with previous studies conducted in other types of epithelial cancer, that ovarian cancer cells are generally softer and display lower intrinsic variability in cell stiffness than non-malignant ovarian epithelial cells. A detailed examination of highly invasive ovarian cancer cells (HEY A8) relative to their less invasive parental cells (HEY), demonstrates that deformability is also an accurate biomarker of metastatic potential. Comparative gene expression analyses indicate that the reduced stiffness of highly metastatic HEY A8 cells is associated with actin cytoskeleton remodeling and microscopic examination of actin fiber structure in these cell lines is consistent with this prediction. Our results indicate that cell stiffness may be a useful biomarker to evaluate the relative metastatic potential of ovarian and perhaps other types of cancer cells.read more
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Conformal piezoelectric systems for clinical and experimental characterization of soft tissue biomechanics.
Canan Dagdeviren,Canan Dagdeviren,Yan Shi,Yan Shi,Pauline Joe,Roozbeh Ghaffari,Guive Balooch,Karan Usgaonkar,Onur Gur,Phat L. Tran,Jessica R. Crosby,Marcin Meyer,Yewang Su,Yewang Su,R. Chad Webb,Andrew S. Tedesco,Marvin J. Slepian,Yonggang Huang,John A. Rogers +18 more
TL;DR: Developing conformal and piezoelectric devices that enable in vivo measurements of soft tissue viscoelasticity in the near-surface regions of the epidermis for rapid and non-invasive characterization of skin mechanical properties.
Journal ArticleDOI
Characterizing deformability and surface friction of cancer cells
Sangwon Byun,Sungmin Son,Dario Amodei,Nathan Cermak,Josephine Shaw,Joon Ho Kang,Vivian C. Hecht,Monte M. Winslow,Monte M. Winslow,Tyler Jacks,Parag Mallick,Scott R. Manalis +11 more
TL;DR: A device that enables the precise measurement of the size and velocity of a single cell, given by its buoyant mass, and the velocity of the cell as it transits through the constriction, suggesting that these parameters can give insight into the factors affecting the passage of each cell.
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Discrimination Between Normal and Cancerous Cells Using AFM
TL;DR: The AFM studies directly comparing the mechanical properties of normal and cancerous cells are summarized and presented together with a few important issues related to the relativeness of Young’s modulus.
Journal ArticleDOI
Cytotoxic T Cells Use Mechanical Force to Potentiate Target Cell Killing.
Roshni Basu,Benjamin M. Whitlock,Julien Husson,Audrey Le Floc’h,Weiyang Jin,Alon Oyler-Yaniv,Farokh Dotiwala,Grégory Giannone,Claire Hivroz,Nicolas Biais,Judy Lieberman,Lance C. Kam,Morgan Huse +12 more
TL;DR: An unappreciated physical dimension to lymphocyte function is revealed and cells use mechanical forces to control the activity of outgoing chemical signals and data indicate that CTLs coordinate perforin release and force exertion in space and time.
Journal ArticleDOI
Mechanical forces in the immune system
TL;DR: The current understanding of how mechanical force regulates cell-surface receptor activation, cell migration, intracellular signalling and intercellular communication is reviewed, highlighting the biological ramifications of these effects in various immune cell types.
References
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Journal Article
R: A language and environment for statistical computing.
TL;DR: Copyright (©) 1999–2012 R Foundation for Statistical Computing; permission is granted to make and distribute verbatim copies of this manual provided the copyright notice and permission notice are preserved on all copies.
Journal ArticleDOI
Significance analysis of microarrays applied to the ionizing radiation response
TL;DR: A method that assigns a score to each gene on the basis of change in gene expression relative to the standard deviation of repeated measurements is described, suggesting that this repair pathway for UV-damaged DNA might play a previously unrecognized role in repairing DNA damaged by ionizing radiation.
Journal ArticleDOI
Cancer statistics, 2010
TL;DR: The American Cancer Society as mentioned in this paper estimated the number of new cancer cases and deaths expected in the United States in the current year and compiles the most recent data regarding cancer incidence, mortality, and survival based on incidence data from the National Cancer Institute, the Centers for Disease Control and Prevention, and the North American Association of Central Cancer Registries and mortality data from National Center for Health Statistics.
Journal ArticleDOI
Calibration of atomic‐force microscope tips
TL;DR: In this article, the authors describe a simple, nondestructive procedure for measuring the force constant, resonant frequency, and quality factor of an AFM cantilever spring and the effective radius of curvature of an AU tip.
Journal ArticleDOI
Cell mechanics and the cytoskeleton
TL;DR: An important insight emerging from this work is that long-lived cytoskeletal structures may act as epigenetic determinants of cell shape, function and fate.