Chromosome number variation in three mouse embryonic stem cell lines during culture
TLDR
The results remark the necessity to perform the karyotype analysis during culture in order to develop new culture conditions to maintain the correct chromosome complement in long-term culture of mESC lines.Abstract:
Although mouse embryonic stem cell lines (mESCs) have been established since 1981, systematic studies about chromosomal changes during culture are lacking. In this study, we report the results of a cytogenetic analysis performed on three mESC lines (named UPV02, UPV06 and UPV08) cultured for a period of 3 months. At time intervals, the variation of the chromosome number together with the expression of markers of the undifferentiated status, i.e., OCT-4, SSEA-1, FOM-1 and alkaline phosphatase activity, were determined. The three mESC lines showed a progressive loss of euploid metaphases during the 3 months period of culture. Chromosome abnormalities were accumulated at the latest passages analysed. Metacentric chromosomes were the most frequent chromosome abnormality observed throughout the period of culture. Interestingly, in coincidence with, or few passages after, the drop of euploidy, the alkaline phosphatase activity was partially or totally lost, whereas the OCT-4, SSEA-1 and FOM-1 stem markers were always positive throughout the period of culture. Our results remark the necessity to perform the karyotype analysis during culture in order to develop new culture conditions to maintain the correct chromosome complement in long-term culture of mESC lines.read more
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Zscan4 regulates telomere elongation and genomic stability in ES cells
Michal Zalzman,Geppino Falco,Lioudmila V. Sharova,Akira Nishiyama,Marshall Thomas,Sung Lim Lee,Carole A. Stagg,Hien G. Hoang,Hsih Te Yang,Fred E. Indig,Robert P. Wersto,Minoru S.H. Ko +11 more
TL;DR: Zscan4 knockdown shortens telomeres, increases karyotype abnormalities and spontaneous sister chromatid exchange, and slows down cell proliferation until reaching crisis by passage eight, which shows a unique mode of genome maintenance in ES cells.
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ES cell cycle progression and differentiation require the action of the histone methyltransferase Dot1L
Evan Barry,Winfried Krueger,Caroline M. Jakuba,Eric S Veilleux,Dominic J. Ambrosi,Craig E. Nelson,Theodore P. Rasmussen +6 more
TL;DR: The histone methyltransferase Dot1L is responsible for methylation of histone H3 at lysine 79 (H3K79me) in mouse embryonic stem cells as discussed by the authors.
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Emerging strategies for cell and gene therapy of the muscular dystrophies.
TL;DR: The therapeutic developments for Duchenne muscular dystrophy is focused on as a model of the types of approaches being considered for various types of dystrophies, as well as prospects and recent successes in the context of future clinical applications.
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C-MYC Transcriptionally Amplifies SOX2 Target Genes to Regulate Self-Renewal in Multipotent Otic Progenitor Cells
TL;DR: It is proposed that SOX2 and C-MYC regulate cell-cycle progression of these cells and that downregulation of C- MYC expression after growth factor withdrawal serves as a molecular switch for differentiation.
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SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy
John P. Didion,Ryan J. Buus,Zohreh Naghashfar,David W. Threadgill,Herbert C. Morse,Fernando Pardo-Manuel de Villena +5 more
TL;DR: The results demonstrate that SNP array profiling is an effective method to combat cell line misidentification and may have substantial implications for studies that are dependent on the expected background of their cell cultures.
References
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Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
Establishment in culture of pluripotential cells from mouse embryos
TL;DR: The establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts are reported, able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo.
Journal ArticleDOI
Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
TL;DR: In this article, the authors described the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice and demonstrated the pluripotency of these embryonic stem cells by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types.
Journal ArticleDOI
Altered cell cycle arrest and gene amplification potential accompany loss of wild-type p53.
Laura R. Livingstone,Alicia White,Alicia White,Jason Sprouse,Jason Sprouse,Elizabeth Livanos,Elizabeth Livanos,Tyler Jacks,Thea D. Tlsty,Thea D. Tlsty +9 more
TL;DR: Loss of wild-type p53 may lead to amplification, possibly caused by changes in cell cycle progression, since tumor cells with wild- type p53 have the ability to amplify genes.
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