Journal ArticleDOI
Delimitation and properties of DNA sequence: required for the regulated expression of human interferon-β gene
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TLDR
Estimation study of mutant IFN-beta genes demonstrates that sequences upstream, but not downstream, of -40 from the cap site are responsible for the viral induction of the gene, and the upstream boundary of the DNA sequences required to support the maximum level of induction lies between -117 and -105 from the caps.About:
This article is published in Cell.The article was published on 1985-06-01. It has received 178 citations till now. The article focuses on the topics: Upstream activating sequence & Regulation of gene expression.read more
Citations
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Journal ArticleDOI
Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor.
Peter Angel,Masayoshi Imagawa,Robert Chiu,Bernd Stein,Richard J. Imbra,Hans J. Rahmsdorf,Carsten Jonat,Peter Herrlich,Michael Karin +8 more
TL;DR: Results strongly suggest that AP-1 is at the receiving end of a complex pathway responsible for transmitting the effects of phorbol ester tumor promoters from the plasma membrane to the transcriptional machinery.
Journal ArticleDOI
Interferons, interferon‐like cytokines, and their receptors
TL;DR: This review summarizes the history of the purification of human IFNs and the key aspects of the current state of knowledge of humanIFN genes, proteins, and receptors and provides some new insights into the development of these proteins as major elements of innate immunity.
Journal ArticleDOI
Regulation of inducible and tissue-specific gene expression
TL;DR: A number of cases of inducible and tissue-specific gene expression involve the activation of preexisting transcription factors, rather than the synthesis of new proteins, which may involve covalent modification of the protein or an allosteric change in its structure.
Journal ArticleDOI
IRF family of transcription factors as regulators of host defense
TL;DR: Their functional roles, through interactions with their own or other members of the family of transcription factors, are becoming clearer in the regulation of host defense, such as innate and adaptive immune responses and oncogenesis.
Journal ArticleDOI
Regulated expression of a gene encoding a nuclear factor, IRF-1, that specifically binds to IFN-β gene regulatory elements
Masaaki Miyamoto,Takashi Fujita,Yoko Kimura,Mitsuo Maruyama,Hisashi Harada,Yoshiaki Sudo,Takashi Miyata,Tadatsugu Taniguchi +7 more
TL;DR: It is shown here that the IRF-1 gene possesses virus-inducible promoter and is also involved in the regulation of other genes such as IFN-alpha and MHC class I genes.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
Journal ArticleDOI
Expression of a β-globin gene is enhanced by remote SV40 DNA sequences
TL;DR: The activation of genes by specific enhancer elements seems to be a widespread mechanism that may be used for the regulation of gene expression and defines a class of DNA elements with a mode of action that has not been heretofore described.
Journal ArticleDOI
Transformation of mammalian cells with genes from procaryotes and eucaryotes
Michael Wigler,Raymond W. Sweet,Gek Kee Sim,Barbara Wold,A. Pellicer,Elizabeth Lacy,Tom Maniatis,Saul Silverstein,Richard Axel +8 more
TL;DR: This co-transformation system should allow the introduction and stable integration of virtually any defined gene into cultured cells and is likely to represent a subpopulation of competent cells which are likely to integrate other unlinked genes at frequencies higher than the general population.
Journal ArticleDOI
In vivo sequence requirements of the SV40 early promoter region
TL;DR: To investigate the sequences necessary for proper initiation of transcription of SV40 early genes, several deletion mutants are constructed in the promoter region of the TATA box region.