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Journal ArticleDOI

Detection and identification of opportunistic Exophiala species using the rolling circle amplification of ribosomal internal transcribed spacers.

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TLDR
Rolling circle amplification (RCA) is applied to species of Exophiala known to cause systemic infections in humans and proved to successfully amplify DNA of the target fungi at the level of species; while no cross-reactivity was observed.
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This article is published in Journal of Microbiological Methods.The article was published on 2013-09-01. It has received 44 citations till now. The article focuses on the topics: Exophiala & Rolling circle replication.

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Citations
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Fungal diversity notes 111–252—taxonomic and phylogenetic contributions to fungal taxa

Guo Jie Li, +164 more
- 23 May 2016 - 
TL;DR: This paper is a compilation of notes on 142 fungal taxa, including five new families, 20 new genera, and 100 new species, representing a wide taxonomic and geographic range.
Journal ArticleDOI

Identification of horizontally transferred genes in the genus Colletotrichum reveals a steady tempo of bacterial to fungal gene transfer.

TL;DR: A constant flux of genes from bacteria to fungi throughout the evolution of subphylum Pezizomycotina is revealed, occurring at a steady rate during their evolution.
Journal ArticleDOI

Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification.

TL;DR: In this article, rolling circle amplification (RCA) was used for species-specific identification of human-pathogenic Sporothrix infection using padlock probes targeting polymorphisms in the gene encoding calmodulin.
Journal ArticleDOI

Identification of Mycoses in Developing Countries.

TL;DR: Next-generation sequencing and other innovative technologies such as clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostic tools may lead to efficient, alternate methods that can be used in point-of-care assays, which may supplement or replace some of the current technologies and improve the diagnostics of fungal infections in developing countries.
Journal ArticleDOI

Rapid Identification of Black Grain Eumycetoma Causative Agents Using Rolling Circle Amplification

TL;DR: A rapid, simple, and highly efficient molecular based method for identification of agents of black grain eumycetoma is introduced, aiming to improve diagnostic in endemic areas and is highly reproducible and can be performed within a single day.
References
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Journal ArticleDOI

Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi

Conrad L. Schoch, +160 more
TL;DR: Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probability of successful identification for the broadest range of fungi, with the most clearly defined barcode gap between inter- and intraspecific variation.
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Melanized Fungi in Human Disease

TL;DR: Though they are uncommon causes of disease, melanized fungi have been increasingly recognized as important pathogens, with most reports occurring in the past 20 years, and the spectrum of diseases with which they are associated has also broadened.
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Occurrence and relevance of filamentous fungi in respiratory secretions of patients with cystic fibrosis – a review

TL;DR: In CF patients, fungi may sometimes be responsible for deterioration of lung function, as occurs in allergic broncho-pulmonary aspergillosis (ABPA), which is the most common fungal disease in this context.
Journal ArticleDOI

Spectrum of Clinically Relevant Exophiala Species in the United States

TL;DR: The re-identified 188 clinical isolates from the United States, which had a preliminary morphological identification of Exophiala species, were re- identified, by sequencing internal transcribed spacer (ITS) region of the rRNA.
Journal ArticleDOI

Dishwashers--a man-made ecological niche accommodating human opportunistic fungal pathogens.

TL;DR: It is concluded that high temperature, high moisture and alkaline pH values typically occurring in dishwashers can provide an alternative habitat for species also known to be pathogenic to humans.
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