Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
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TLDR
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.Abstract:
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium (ectoderm). These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine.read more
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Journal ArticleDOI
Prospective identification of cardiac progenitors by a novel single cell-based cardiomyocyte induction
Jun K. Yamashita,Jun K. Yamashita,Makoto Takano,Mina Hiraoka-Kanie,Mina Hiraoka-Kanie,Chikashi Shimazu,Yan Peishi,Kentoku Yanagi,Akiko Nakano,Emi Inoue,Fumiyo Kita,Shin-Ichi Nishikawa +11 more
TL;DR: It is shown that cardiomyocytes can be successfully induced from a single Flk1+ cell on 2‐dimensional culture, enabling the direct observation of differentiating cardiomers and the prospective identification of cardiac progenitor potentials.
Book ChapterDOI
Preparation of Magnetically Labeled Cells for Cell Tracking by Magnetic Resonance Imaging
TL;DR: This chapter describes the preparation of magnetically labeled cells for cell tracking by magnetic resonance imaging (MRI), and a significant improvement of labeling of nonphagocytic cells has been achieved by linking the particles to the human immunodeficiency virus (HIV) tat peptide.
Journal ArticleDOI
Large-scale expansion of pluripotent human embryonic stem cells in stirred-suspension bioreactors.
Roman Krawetz,Jaymi T. Taiani,Shiying Liu,Guoliang Meng,Xiangyun Li,Michael S. Kallos,Derrick E. Rancourt +6 more
TL;DR: The use of bioreactors enables the expansion of hES cells in the absence of feeder layers or matrices, which will facilitate the adaptation of good manufacturing process (GMP) standards to the development of h ES cell therapies.
Journal ArticleDOI
Critical microcarrier properties affecting the expansion of undifferentiated human embryonic stem cells.
TL;DR: Conventional microcarriers intended for propagation of mammalian cells are not suitable for long term propagation of hESC, so laminin coatedmicrocarriers (DE53 and Cytodex 1) are capable of supporting the longterm propagation and pluripotency of HES-2 and H ES-3 cell lines.
Journal ArticleDOI
Human oligodendrocytes from embryonic stem cells: conserved SHH signaling networks and divergent FGF effects.
TL;DR: It is shown that sonic hedgehog-dependent sequential activation of the transcription factors OLIG2, NKX2.2 and SOX10 is required for sequential specification of ventral spinal OLig2-expressing progenitors, pre-oligodendrocyte precursor cells and OPCs from hESC-derived neuroepithelia, indicating that a conserved transcriptional network underlies OPC specification in human as in other vertebrates.
References
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Journal ArticleDOI
Establishment in culture of pluripotential cells from mouse embryos
TL;DR: The establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts are reported, able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
TL;DR: In this article, the authors described the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice and demonstrated the pluripotency of these embryonic stem cells by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types.
Journal ArticleDOI
Extension of life-span by introduction of telomerase into normal human cells
Andrea G. Bodnar,Michel M. Ouellette,Maria Frolkis,Shawn E. Holt,Choy-Pik Chiu,Gregg B. Morin,Calvin B. Harley,Jerry W. Shay,Serge Lichtsteiner,Woodring E. Wright +9 more
TL;DR: In this article, two telomerase-negative normal human cell types, retinal pigment epithelial cells and foreskin fibroblasts, were transfected with vectors encoding the human telomere catalytic subunit.
Journal ArticleDOI
Telomere length predicts replicative capacity of human fibroblasts.
Richard C. Allsopp,Homayoun Vaziri,Christopher Patterson,S Goldstein,Edward V. Younglai,A B Futcher,Carol W. Greider,Calvin B. Harley +7 more
TL;DR: Telomere length is a biomarker of somatic cell aging in humans and is consistent with a causal role for telomere loss in this process, and fibroblasts from Hutchinson-Gilford progeria donors had short telomeres, consistent with their reduced division potential in vitro.