Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
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TLDR
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.Abstract:
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium (ectoderm). These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine.read more
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Human embryonic stem cells express a unique set of microRNAs
Mi-Ra Suh,Yoontae Lee,Jung Yeon Kim,Soo-Kyoung Kim,Sung-Hwan Moon,Ji Yeon Lee,Cha Kwang-Yul,Hyung-Min Chung,Hyun Soo Yoon,Shin Yong Moon,V. Narry Kim,Kye Seong Kim +11 more
TL;DR: It is shown that some of the ES-specific miRNA genes are highly related to each other, organized as clusters, and transcribed as polycistronic primary transcripts, suggesting that they may operate key regulatory networks conserved in mammalian pluripotent stem cells.
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Human embryonic stem cells express an immunogenic nonhuman sialic acid
TL;DR: Levels of Neu5Gc on HESC and embryoid bodies dropped after culture in heat-inactivated anti-Neu5 Gc antibody–negative human serum, reducing binding of antibodies and complement from high-titer sera, while allowing maintenance of the undifferentiated state.
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Human mesenchymal stem cells - current trends and future prospective
TL;DR: Recent research findings in the area of hMSCs sources, expression of cell surface markers, long-term in vitro culturing, in vitro differentiation potential, immunomodulatory features, its homing capacity, banking and cryopreservation, its application in the treatment of chronic diseases and its use in clinical trials are highlighted.
Journal ArticleDOI
Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines
Christoph Bock,Evangelos Kiskinis,Evangelos Kiskinis,Griet Verstappen,Griet Verstappen,Hongcang Gu,Gabriella L. Boulting,Zachary D. Smith,Zachary D. Smith,Michael J. Ziller,Michael J. Ziller,Gist F. Croft,Mackenzie W. Amoroso,Derek H. Oakley,Andreas Gnirke,Kevin Eggan,Kevin Eggan,Alexander Meissner,Alexander Meissner +18 more
TL;DR: This resource enabled us to assess the epigenetic and transcriptional similarity of ES and iPS cells and to predict the differentiation efficiency of individual cell lines, and yields a scorecard for quick and comprehensive characterization of pluripotent cell lines.
Journal ArticleDOI
Derivation of novel human ground state naive pluripotent stem cells
Ohad Gafni,Leehee Weinberger,Abed AlFatah Mansour,Yair S. Manor,Elad Chomsky,Dalit Ben-Yosef,Yael Kalma,Sergey Viukov,Itay Maza,Asaf Zviran,Yoach Rais,Zohar Shipony,Zohar Mukamel,Vladislav Krupalnik,Mirie Zerbib,Shay Geula,Inbal Caspi,Dan Schneir,Tamar Shwartz,Shlomit Gilad,Daniela Amann-Zalcenstein,Sima Benjamin,Ido Amit,Amos Tanay,Rada Massarwa,Noa Novershtern,Jacob H. Hanna +26 more
TL;DR: The novel naive pluripotent cells validated herein retain molecular characteristics and functional properties that are highly similar to mouse naive ES cells, and distinct from conventional primed human pluripotency.
References
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Establishment in culture of pluripotential cells from mouse embryos
TL;DR: The establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts are reported, able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo.
Journal ArticleDOI
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Leonard Hayflick,P.S. Moorhead +1 more
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Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
TL;DR: In this article, the authors described the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice and demonstrated the pluripotency of these embryonic stem cells by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types.
Journal ArticleDOI
Extension of life-span by introduction of telomerase into normal human cells
Andrea G. Bodnar,Michel M. Ouellette,Maria Frolkis,Shawn E. Holt,Choy-Pik Chiu,Gregg B. Morin,Calvin B. Harley,Jerry W. Shay,Serge Lichtsteiner,Woodring E. Wright +9 more
TL;DR: In this article, two telomerase-negative normal human cell types, retinal pigment epithelial cells and foreskin fibroblasts, were transfected with vectors encoding the human telomere catalytic subunit.
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Telomere length predicts replicative capacity of human fibroblasts.
Richard C. Allsopp,Homayoun Vaziri,Christopher Patterson,S Goldstein,Edward V. Younglai,A B Futcher,Carol W. Greider,Calvin B. Harley +7 more
TL;DR: Telomere length is a biomarker of somatic cell aging in humans and is consistent with a causal role for telomere loss in this process, and fibroblasts from Hutchinson-Gilford progeria donors had short telomeres, consistent with their reduced division potential in vitro.