Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
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TLDR
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.Abstract:
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium (ectoderm). These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine.read more
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Advanced biomaterials for skeletal tissue regeneration: Instructive and smart functions
TL;DR: Recent developments in the design of biomaterials that integrate the understanding of cellular and molecular mechanisms with materials science are reviewed, including those that can activate cellular processes and tissue formation solely by their intrinsic physicochemical and three dimensional spatial properties.
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Human Fetal Membranes: A Source of Stem Cells for Tissue Regeneration and Repair?
TL;DR: This review will focus on the stem cell like properties of stromal and epithelial cells derived from human fetal membranes and their potential use in stem cell based therapies.
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A negative feedback loop of transcription factors that controls stem cell pluripotency and self-renewal
TL;DR: Evidence is presented that Oct4, Nanog, and FoxD3 form a negative feedback loop to maintain their expression in pluripotent ES cells and an interdependent network of transcription factors that regulate stem cell pluripotency and self‐renewal.
Journal ArticleDOI
Effects of three-dimensional culture and growth factors on the chondrogenic differentiation of murine embryonic stem cells.
Nathaniel S. Hwang,Myoung Sook Kim,Somponnat Sampattavanich,Jin Hyen Baek,Zijun Zhang,Jennifer H. Elisseeff +5 more
TL;DR: It is hypothesized that a supportive three‐dimensional environment provides ES cell‐derived cells an environment that more closely mimics chondrogenesis in vivo, and suggests that EB‐PEG hydrogel culture, with an appropriate growth factor, may provide a suitable environment forChondrogenic differentiation of intact ES cell-derived EBs.
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Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells
Jin Zhang,Esther Nuebel,Dona R.R. Wisidagama,Kiyoko Setoguchi,Jason S. Hong,Christine M Van Horn,Sarah S Imam,Laurent Vergnes,Cindy S. Malone,Carla M. Koehler,Michael A. Teitell +10 more
TL;DR: Protocols for analyzing energy metabolism in hPSCs and their early differentiated progenies that are generally applicable to mature cell types as well are provided and Companion methods are provided to aid researchers in developing more sophisticated experimental regimens for extended analyses of cellular bioenergetics.
References
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Establishment in culture of pluripotential cells from mouse embryos
TL;DR: The establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts are reported, able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo.
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The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
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Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
TL;DR: In this article, the authors described the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice and demonstrated the pluripotency of these embryonic stem cells by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types.
Journal ArticleDOI
Extension of life-span by introduction of telomerase into normal human cells
Andrea G. Bodnar,Michel M. Ouellette,Maria Frolkis,Shawn E. Holt,Choy-Pik Chiu,Gregg B. Morin,Calvin B. Harley,Jerry W. Shay,Serge Lichtsteiner,Woodring E. Wright +9 more
TL;DR: In this article, two telomerase-negative normal human cell types, retinal pigment epithelial cells and foreskin fibroblasts, were transfected with vectors encoding the human telomere catalytic subunit.
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Telomere length predicts replicative capacity of human fibroblasts.
Richard C. Allsopp,Homayoun Vaziri,Christopher Patterson,S Goldstein,Edward V. Younglai,A B Futcher,Carol W. Greider,Calvin B. Harley +7 more
TL;DR: Telomere length is a biomarker of somatic cell aging in humans and is consistent with a causal role for telomere loss in this process, and fibroblasts from Hutchinson-Gilford progeria donors had short telomeres, consistent with their reduced division potential in vitro.