Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
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TLDR
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.Abstract:
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium (ectoderm). These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine.read more
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Analysis of DNA Methylation in a Three-Generation Family Reveals Widespread Genetic Influence on Epigenetic Regulation
Jason Gertz,Katherine E. Varley,Timothy E. Reddy,Kevin M. Bowling,Florencia Pauli,Stephanie L. Parker,Katerina S. Kucera,Huntington F. Willard,Richard M. Myers +8 more
TL;DR: It is found that 75% of genotype-dependent differential methylation events in the family are also seen in unrelated individuals and that overall genotype can explain 80% of the variation in DNA methylation.
Journal ArticleDOI
OCT4 spliced variants are differentially expressed in human pluripotent and nonpluripotent cells.
TL;DR: A distinctive expression pattern for OCT4 spliced variants in different cell types is demonstrated and the necessity of defining the type of OCT4 when addressing the expression of this gene in different human cells is highlighted.
Journal ArticleDOI
Pluripotency in the Embryo and in Culture
Jennifer Nichols,Austin Smith +1 more
TL;DR: The proposition that early epiblast cells and ESCs may represent a naïve ground state without any prespecification of lineage choice, whereas later epiblasts and EpiSCs may be primed in favor of particular fates is considered.
Journal ArticleDOI
Electrospun scaffolds for stem cell engineering.
Shawn H. Lim,Hai-Quan Mao +1 more
TL;DR: The feasibility of using Electrospun fibrous scaffolds to present integrated topographical and biochemical signals that are essential to stem cell manipulation has been demonstrated and future application of this versatile scaffold platform to human embryonic and induced pluripotent stem cells for functional tissue repair and regeneration will further expand its potential for regenerative therapies.
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Transgenic Monkeys Produced by Retroviral Gene Transfer into Mature Oocytes
TL;DR: In this article, the green fluorescent protein (GFP) transgene was detected by both direct and indirect fluorescence imaging from a fraternal set of twins, miscarried at 73 days.
References
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Establishment in culture of pluripotential cells from mouse embryos
TL;DR: The establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts are reported, able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo.
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The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
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Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
TL;DR: In this article, the authors described the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice and demonstrated the pluripotency of these embryonic stem cells by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types.
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Extension of life-span by introduction of telomerase into normal human cells
Andrea G. Bodnar,Michel M. Ouellette,Maria Frolkis,Shawn E. Holt,Choy-Pik Chiu,Gregg B. Morin,Calvin B. Harley,Jerry W. Shay,Serge Lichtsteiner,Woodring E. Wright +9 more
TL;DR: In this article, two telomerase-negative normal human cell types, retinal pigment epithelial cells and foreskin fibroblasts, were transfected with vectors encoding the human telomere catalytic subunit.
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Telomere length predicts replicative capacity of human fibroblasts.
Richard C. Allsopp,Homayoun Vaziri,Christopher Patterson,S Goldstein,Edward V. Younglai,A B Futcher,Carol W. Greider,Calvin B. Harley +7 more
TL;DR: Telomere length is a biomarker of somatic cell aging in humans and is consistent with a causal role for telomere loss in this process, and fibroblasts from Hutchinson-Gilford progeria donors had short telomeres, consistent with their reduced division potential in vitro.