Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
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TLDR
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.Abstract:
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium (ectoderm). These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine.read more
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Silicon micromachining to tissue engineer branched vascular channels for liver fabrication
Satoshi Kaihara,Jeffrey T. Borenstein,Rahul Koka,Sonal Lalan,Erin R. Ochoa,Michael Ravens,Homer Pien,Brian T. Cunningham,Joseph P. Vacanti,Joseph P. Vacanti +9 more
TL;DR: Preliminary results using micromachining technologies on silicon and Pyrex surfaces to generate complete vascular systems that may be integrated with engineered tissue before implantation are reported, with the use microfabrication technology in tissue engineering.
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Wnt Signaling Controls the Specification of Definitive and Primitive Hematopoiesis From Human Pluripotent Stem Cells
TL;DR: It is shown that both hematopoietic programs transition through hemogenic endothelial intermediates and develop from KDR+CD34−CD144− progenitors that are distinguished by CD235a expression.
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Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrification method.
TL;DR: Vitrified human ES cells were cultivated for prolonged periods and retained the properties of pluripotent cells, including a normal karyotype, expression of the transcription factor Oct-4 and surface markers that are characteristic tohuman ES cells.
Journal ArticleDOI
Increased cardiomyocyte differentiation from human embryonic stem cells in serum-free cultures.
Robert Passier,Dorien Ward-van Oostwaard,Jolanda Snapper,Jantine Kloots,Rutger J. Hassink,Ewart W. Kuijk,Bernard A.J. Roelen,Aart Brutel de la Rivière,Christine L. Mummery +8 more
TL;DR: A striking inverse relationship between cardiomyocyte differentiation and the concentration of FCS is demonstrated during HES‐2‐END‐2 coculture, and this model represents an improved in vitro model, without interfering factors in serum, for testing other factors that might promote cardiomeocyte differentiation.
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Antioxidative stress–associated genes in circulating progenitor cells: evidence for enhanced resistance against oxidative stress
Elisabeth Dernbach,Carmen Urbich,Ralf P. Brandes,Wolf K. Hofmann,Andreas M. Zeiher,Stefanie Dimmeler +5 more
TL;DR: EPCs reveal a higher expression of antioxidative enzymes and are exquisitely equipped to be protected against oxidative stress consistent with their progenitor cell character, and combined inhibition of these antioxidants increased ROS levels in EPCs and impaired EPC survival and migration.
References
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Establishment in culture of pluripotential cells from mouse embryos
TL;DR: The establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts are reported, able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo.
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The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
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Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
TL;DR: In this article, the authors described the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice and demonstrated the pluripotency of these embryonic stem cells by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types.
Journal ArticleDOI
Extension of life-span by introduction of telomerase into normal human cells
Andrea G. Bodnar,Michel M. Ouellette,Maria Frolkis,Shawn E. Holt,Choy-Pik Chiu,Gregg B. Morin,Calvin B. Harley,Jerry W. Shay,Serge Lichtsteiner,Woodring E. Wright +9 more
TL;DR: In this article, two telomerase-negative normal human cell types, retinal pigment epithelial cells and foreskin fibroblasts, were transfected with vectors encoding the human telomere catalytic subunit.
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Telomere length predicts replicative capacity of human fibroblasts.
Richard C. Allsopp,Homayoun Vaziri,Christopher Patterson,S Goldstein,Edward V. Younglai,A B Futcher,Carol W. Greider,Calvin B. Harley +7 more
TL;DR: Telomere length is a biomarker of somatic cell aging in humans and is consistent with a causal role for telomere loss in this process, and fibroblasts from Hutchinson-Gilford progeria donors had short telomeres, consistent with their reduced division potential in vitro.