Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
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TLDR
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.Abstract:
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium (ectoderm). These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine.read more
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Magnetic intracellular labeling of mammalian cells by combining (FDA-approved) superparamagnetic iron oxide MR contrast agents and commonly used transfection agents.
Joseph A. Frank,Holly A. Zywicke,Elaine K. Jordan,Jennifer Mitchell,Bobbi K. Lewis,Brad Miller,L. Henry Bryant,Jeff W.M. Bulte +7 more
TL;DR: The magnetic labeling results of combining dendrimers and other commercially available TA’s with (FDA-approved) dextran-coated iron oxide MR contrast agents (Feridex and MION-46L) are presented.
Journal ArticleDOI
Glycolytic Metabolism Plays a Functional Role in Regulating Human Pluripotent Stem Cell State
Wen Gu,Xavier Gaeta,Anna Sahakyan,Alanna B. Chan,Candice S. Hong,Rachel Kim,Daniel Braas,Kathrin Plath,William E. Lowry,Heather R. Christofk +9 more
TL;DR: In this paper, the rate of glycolytic metabolism changes during differentiation of human embryonic stem cells (hESCs) and reprogramming of somatic cells to pluripotency.
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Embryonic stem (ES) cells and embryonal carcinoma (EC) cells: opposite sides of the same coin.
Peter W. Andrews,Maryam Moghaddam Matin,Ahmad Reza Bahrami,Ivan Damjanov,Paul J. Gokhale,Jonathan S. Draper +5 more
TL;DR: It is suggested that ES cells may develop in culture in ways that mimic changes occurring in EC cells during tumour progression, and this work obtained a teratocarcinoma containing histologically recognizable stem cells.
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Cancer Stem Cell Hierarchy in Glioblastoma Multiforme
TL;DR: Current knowledge on the CSC markers SALL4, OCT-4, SOX2, STAT3, NANOG, c-Myc, KLF4, CD133, CD44, nestin, and glial fibrillary acidic protein are examined, specifically focusing on their use and validity in GBM research and how they may be utilized for investigations into GBM’s cancer biology.
Journal ArticleDOI
Improved viability of random pattern skin flaps through the use of adipose-derived stem cells.
TL;DR: Findings suggest that adipose-derived stem cells have a potential for enhancing the blood supply of random pattern skin flaps and might be both the direct differentiation of adipose stem cells into endothelial cells and the indirect effect of angiogenic growth factor released from adipose.
References
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Establishment in culture of pluripotential cells from mouse embryos
TL;DR: The establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts are reported, able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo.
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The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
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Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
TL;DR: In this article, the authors described the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice and demonstrated the pluripotency of these embryonic stem cells by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types.
Journal ArticleDOI
Extension of life-span by introduction of telomerase into normal human cells
Andrea G. Bodnar,Michel M. Ouellette,Maria Frolkis,Shawn E. Holt,Choy-Pik Chiu,Gregg B. Morin,Calvin B. Harley,Jerry W. Shay,Serge Lichtsteiner,Woodring E. Wright +9 more
TL;DR: In this article, two telomerase-negative normal human cell types, retinal pigment epithelial cells and foreskin fibroblasts, were transfected with vectors encoding the human telomere catalytic subunit.
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Telomere length predicts replicative capacity of human fibroblasts.
Richard C. Allsopp,Homayoun Vaziri,Christopher Patterson,S Goldstein,Edward V. Younglai,A B Futcher,Carol W. Greider,Calvin B. Harley +7 more
TL;DR: Telomere length is a biomarker of somatic cell aging in humans and is consistent with a causal role for telomere loss in this process, and fibroblasts from Hutchinson-Gilford progeria donors had short telomeres, consistent with their reduced division potential in vitro.