Journal ArticleDOI
Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects.
TLDR
Progress in the development of probes for "reactive oxygen and nitrogen" species, emphasizing the caution needed in their use is reviewed, with a focus on probes based on reduced dyes.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2007-10-01. It has received 804 citations till now. The article focuses on the topics: Reactive nitrogen species & Radical.read more
Citations
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Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
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Design strategies for water-soluble small molecular chromogenic and fluorogenic probes.
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Thiol chemistry and specificity in redox signaling.
TL;DR: This review focuses on mechanisms for sensing and transmitting redox signals, from the perspective of their chemical reactivity with specific oxidants, and discusses substrate preferences for different oxidants and how the kinetics of these reactions determines how each oxidant will react in a cell.
Journal ArticleDOI
Plasma-liquid interactions: A review and roadmap
Peter Bruggeman,Mark J. Kushner,Bruce R. Locke,Jge Gardeniers,William Graham,David B. Graves,Rchm Hofman-Caris,D Marić,Jonathan P. Reid,E Ceriani,D. Fernandez Rivas,John E. Foster,Sean C. Garrick,Yury Gorbanev,Satoshi Hamaguchi,Felipe Iza,Helena Jablonowski,E. Klimova,Juergen F. Kolb,František Krčma,Petr Lukes,Zdenko Machala,Ilya Marinov,Davide Mariotti,S. Mededovic Thagard,Daisuke Minakata,Erik C. Neyts,Joanna Pawłat,Z. Lj. Petrović,R Pflieger,Stephan Reuter,DC Daan Schram,Sandra Schröter,Manabu Shiraiwa,Barbora Tarabová,Pa Tsai,Jrr Verlet,T. von Woedtke,Kevin R. Wilson,Kyuichi Yasui,G. Zvereva +40 more
TL;DR: A review of the state-of-the-art of this multidisciplinary area and identifying the key research challenges is provided in this paper, where the developments in diagnostics, modeling and further extensions of cross section and reaction rate databases are discussed.
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The sites and topology of mitochondrial superoxide production
TL;DR: The relative contribution of each site to mitochondrial reactive oxygen species generation in the absence of electron transport inhibitors is unknown in isolated mitochondria, in cells or in vivo, and may vary considerably with species, tissue, substrate, energy demand and oxygen tension.
References
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Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide.
TL;DR: It is proposed that superoxide dismutase may protect vascular tissue stimulated to produce superoxide and NO under pathological conditions by preventing the formation of peroxynitrite.
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Mitochondrial free radical generation, oxidative stress, and aging.
TL;DR: In this article, the authors reviewed the mitochondrial rates of production and steady state levels of reactive oxygen species generated by mitochondria, or from other sites within or outside the cell, cause damage to mitochondrial components and initiate degradative processes.
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Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe.
TL;DR: The improved ORAC(FL) assay provides a direct measure of hydrophilic chain-breaking antioxidant capacity against peroxyl radical and demonstrates that fluorescein (FL) is superior to B-phycoerythrin.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
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Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?
Barry Halliwell,Matthew Whiteman +1 more
TL;DR: Methods used to trap RS, including spin trapping and aromatic hydroxylation, are critically examined, with a particular emphasis on those methods applicable to human studies and methods used to detect RS in cell culture.