Journal ArticleDOI
Galectin LEC-1 plays a defensive role against damage due to oxidative stress in Caenorhabditis elegans.
Reads0
Chats0
TLDR
It is speculated that interaction of L EC-1 and MIG-6 in the pharynx may affect susceptibility to paraquat and that LEC-10 has different functions from Lec-1 in regulating H2O2 and paraquats resistance in the intestine.Abstract:
LEC-1 is a major galectin in Caenorhabditis elegans and contains two carbohydrate recognition domains (CRDs), N-CRD and C-CRD. To determine the role of LEC-1, we examined the phenotypes of a mutant C. elegans strain lacking lec-1. We observed negligible differences in embryogenesis, morphogenesis and egg laying at 20 °C between the mutant and the wild-type. Furthermore, the life spans of the mutant and the wild-type were equivalent at either 20 °C or 25 °C. However, the lec-1 mutant showed a greater susceptibility to H2O2 and paraquat than the wild-type. This result suggests an increased susceptibility to oxidative stress, with the phenotypes being similar to those of lec-10 deletion mutants as previously described. To understand the molecular mechanism underlying this phenotype, C. elegans proteins bound by either the LEC-1 N-CRD or C-CRD were isolated and identified using a nano liquid chromatography-tandem mass spectrometry technique. MIG-6 was identified as a major binding partner of LEC-1 with both N- and C-CRD. From these results and previous reports, we speculate that interaction of LEC-1 and MIG-6 in the pharynx may affect susceptibility to paraquat and that LEC-10 has different functions from LEC-1 in regulating H2O2 and paraquat resistance in the intestine.read more
Citations
More filters
Journal ArticleDOI
The protein inputs of an ultra-predictive aging clock represent viable anti-aging drug targets.
TL;DR: In this paper, an aging clock was developed that utilizes 491 plasma protein inputs, has an exceptional accuracy, and is capable of measuring biological age. But, the clock was used to measure age in a novel plasma proteomic dataset derived from 370 human subjects aged 18-69 years.
Posted ContentDOI
Establishment of a marine nematode model for animal functional genomics, environmental adaptation and developmental evolution
Yusu Xie,Pengchi Zhang,Beining Xue,Xuwen Cao,Xiaoliang Ren,Lili Wang,Yuanyuan Sun,Hanwen Yang,Liusuo Zhang +8 more
TL;DR: The first inbred line of free-living marine nematode Litoditis marina is constructed, sequenced and assembled its genome, and CRISPR/Cas9 genome editing is successfully applied in L. marina.
Journal ArticleDOI
Galactoseβ1-4fucose: A unique disaccharide unit found in N-glycans of invertebrates including nematodes.
TL;DR: The Galβ1‐4Fuc unit in Protostomia is discussed as a possible equivalent for the Galβ 1‐4GlcNAc unit in vertebrates and a potential non‐self glycomarker useful for pathogen recognition.
Journal ArticleDOI
Global Proteomic Response of Caenorhabditis elegans Against PemKSa Toxin
TL;DR: Assessment of the impact of Staphylococcus aureus toxin on the host C. elegans provided a preliminary view of the physiological and molecular response of a host toward a toxin and provided insight into highly complex host-toxin interactions.
Journal ArticleDOI
A Proteomic Analysis Provides Novel Insights into the Stress Responses of Caenorhabditis elegans towards Nematicidal Cry6A Toxin from Bacillus thuringiensis.
TL;DR: The findings illustrate the global pattern of defense responses of nematode against Cry6A for the first time, and provide a novel insight into the mechanism through which worms respond toCry6A, a novel family of nematicidal crystal proteins from Bacillus thuringiensis.
References
More filters
Journal ArticleDOI
Aging: A Theory Based on Free Radical and Radiation Chemistry
TL;DR: It seems possible that one factor in aging may be related to deleterious side attacks of free radicals (which are normally produced in the course of cellular metabolism) on cell constituents.
Journal ArticleDOI
Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans
Birte Sönnichsen,Liisa Koski,Andrew Walsh,P. Marschall,Beate Neumann,M. Brehm,Anne-Marie Alleaume,J. Artelt,Paulo Bettencourt,Etienne Cassin,M. Hewitson,C. Holz,Moddasar N. Khan,S. Lazik,Charles E. Martin,Björn Nitzsche,Martine Ruer,Joanne Stamford,Maria Winzi,R. Heinkel,Marion S. Röder,J. Finell,H. Häntsch,Steven J.M. Jones,Martin R. Jones,Martin R. Jones,Fabio Piano,Kristin C. Gunsalus,Karen Oegema,Pierre Gönczy,Pierre Gönczy,Alan Coulson,Alan Coulson,Anthony A. Hyman,Christophe Echeverri +34 more
TL;DR: This work used RNA-mediated interference to target 98% of all genes predicted in the C. elegans genome in combination with differential interference contrast time-lapse microscopy and developed a phenotypic profiling system, which shows high correlation with cellular processes and biochemical pathways, thus enabling to predict new functions for previously uncharacterized genes.
Journal ArticleDOI
Oligosaccharide specificity of galectins: a search by frontal affinity chromatography.
Jun Hirabayashi,Tomomi Hashidate,Yoichiro Arata,Nozomu Nishi,Takanori Nakamura,Mitsuomi Hirashima,Tadasu Urashima,Toshihiko Oka,Masamitsu Futai,Werner E.G. Müller,Fumio Yagi,Ken-ichi Kasai +11 more
TL;DR: In this paper, a frontal affinity chromatography (FAC) was used to quantitatively analyze the interactions at 20 °C between 13 galectins including 16 CRDs originating from mammals, chick, nematode, sponge, and mushroom, with 41 pyridylaminated (PA) oligosaccharides.
Journal ArticleDOI
Galectins: structure, function and therapeutic potential.
TL;DR: Current research indicates that galectins play important roles in diverse physiological and pathological processes, including immune and inflammatory responses, tumour development and progression, neural degeneration, atherosclerosis, diabetes, and wound repair, and may be a therapeutic target or employed as therapeutic agents for inflammatory diseases, cancers and several other diseases.
Journal ArticleDOI
Intracellular functions of galectins
TL;DR: This review summarizes the intracellular activities displayed by several galectins and discusses the possible underlying mechanisms.