Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: Implications for the microbial “pan-genome”
Hervé Tettelin,Vega Masignani,Michael J. Cieslewicz,Claudio Donati,Duccio Medini,Naomi L. Ward,Samuel V. Angiuoli,Jonathan Crabtree,Amanda L. Jones,A. Scott Durkin,Robert T. DeBoy,Tanja M. Davidsen,Marirosa Mora,Maria Scarselli,Immaculada Margarit Y Ros,Jeremy Peterson,Christopher R. Hauser,Jaideep P. Sundaram,William C. Nelson,Ramana Madupu,Lauren M. Brinkac,Robert J. Dodson,M. J. Rosovitz,Steven A. Sullivan,Sean C. Daugherty,Daniel H. Haft,Jeremy D. Selengut,Michelle L. Gwinn,Liwei Zhou,Nikhat Zafar,Hoda Khouri,Diana Radune,George Dimitrov,Kisha Watkins,Kevin J. B. O'Connor,Shannon Smith,Teresa Utterback,Owen White,Craig E. Rubens,Guido Grandi,Lawrence C. Madoff,Dennis L. Kasper,John L. Telford,Michael R. Wessels,Rino Rappuoli,Claire M. Fraser +45 more
TLDR
The genomic sequence of six strains representing the five major disease-causing serotypes of Streptococcus agalactiae, the main cause of neonatal infection in humans, was generated and Mathematical extrapolation of the data suggests that the gene reservoir available for inclusion in the S. agalactic pan-genome is vast and that unique genes will continue to be identified even after sequencing hundreds of genomes.Abstract:
The development of efficient and inexpensive genome sequencing methods has revolutionized the study of human bacterial pathogens and improved vaccine design. Unfortunately, the sequence of a single genome does not reflect how genetic variability drives pathogenesis within a bacterial species and also limits genome-wide screens for vaccine candidates or for antimicrobial targets. We have generated the genomic sequence of six strains representing the five major disease-causing serotypes of Streptococcus agalactiae, the main cause of neonatal infection in humans. Analysis of these genomes and those available in databases showed that the S. agalactiae species can be described by a pan-genome consisting of a core genome shared by all isolates, accounting for ≈80% of any single genome, plus a dispensable genome consisting of partially shared and strain-specific genes. Mathematical extrapolation of the data suggests that the gene reservoir available for inclusion in the S. agalactiae pan-genome is vast and that unique genes will continue to be identified even after sequencing hundreds of genomes.read more
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NCBI prokaryotic genome annotation pipeline
Tatiana Tatusova,Michael DiCuccio,Azat Badretdin,Vyacheslav Chetvernin,Eric P. Nawrocki,Leonid Zaslavsky,Alexandre Lomsadze,Kim D. Pruitt,Mark Borodovsky,James Ostell +9 more
TL;DR: The new NCBI's Prokaryotic Genome Annotation Pipeline (PGAP) relies less on sequence similarity when confident comparative data are available, while it relies more on statistical predictions in the absence of external evidence.
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progressiveMauve: Multiple Genome Alignment with Gene Gain, Loss and Rearrangement
TL;DR: A new method to align two or more genomes that have undergone rearrangements due to recombination and substantial amounts of segmental gain and loss is described, demonstrating high accuracy in situations where genomes have undergone biologically feasible amounts of genome rearrangement, segmental loss and loss.
Journal ArticleDOI
IMG: the integrated microbial genomes database and comparative analysis system
Victor M. Markowitz,I-Min A. Chen,Krishna Palaniappan,Ken Chu,Ernest Szeto,Yuri Grechkin,Anna Ratner,Biju Jacob,Jinghua Huang,Peter L. Williams,Marcel Huntemann,Iain Anderson,Konstantinos Mavromatis,Natalia Ivanova,Nikos C. Kyrpides +14 more
TL;DR: The Integrated Microbial Genomes system serves as a community resource for comparative analysis of publicly available genomes in a comprehensive integrated context and provides tools and viewers for analyzing and reviewing the annotations of genes and genomes inA comparative context.
Journal ArticleDOI
Organised Genome Dynamics in the Escherichia coli Species Results in Highly Diverse Adaptive Paths
Marie Touchon,Marie Touchon,Claire Hoede,Olivier Tenaillon,Valérie Barbe,Simon Baeriswyl,Philippe Bidet,Edouard Bingen,Stéphane Bonacorsi,Christiane Bouchier,Odile Bouvet,Alexandra Calteau,Hélène Chiapello,Olivier Clermont,Stéphane Cruveiller,Antoine Danchin,Médéric Diard,Carole Dossat,Meriem El Karoui,Eric Frapy,Louis Garry,Jean Marc Ghigo,Anne-Marie Gilles,James R. Johnson,James R. Johnson,Chantal Le Bouguénec,Mathilde Lescat,Sophie Mangenot,Vanessa Martinez-Jéhanne,Ivan Matic,Xavier Nassif,Sophie Oztas,Marie-Agnès Petit,Christophe Pichon,Zoé Rouy,Claude Saint Ruf,Dominique Schneider,Jérôme Tourret,Benoit Vacherie,David Vallenet,Claudine Médigue,Eduardo P. C. Rocha,Eduardo P. C. Rocha,Erick Denamur +43 more
TL;DR: An important adaptive role for metabolism diversification within group B2 and Shigella strains is found, but few or no extraint intestinal virulence-specific genes are identified, which could render difficult the development of a vaccine against extraintestinal infections.
Journal ArticleDOI
The microbial pan-genome
TL;DR: A decade after the beginning of the genomic era, the question of how genomics can describe a bacterial species has not been fully addressed and the pan-genome, which is composed of a "core genome" containing genes present in all strains, and a "dispensable genome", might be orders of magnitude larger than any single genome.
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