Journal ArticleDOI
Genomic editing opens new avenues for zebrafish as a model for neurodegeneration.
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TLDR
The efficiency of the novel genome editing tools now greatly facilitates knock‐out and also makes knock‐in approaches feasible in zebrafish, which avoids unspecific phenotypes caused by off‐target effects and toxicity as frequently seen in conventional knock‐down approaches.Abstract:
Zebrafish has become a popular model organism to study human diseases We will highlight the advantages and limitations of zebrafish as a model organism to study neurodegenerative diseases and introduce zinc finger nucleases, transcription activator-like effector nucleases, and the recently established clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeat-associated system for genome editing The efficiency of the novel genome editing tools now greatly facilitates knock-out and, importantly, also makes knock-in approaches feasible in zebrafish Genome editing in zebrafish avoids unspecific phenotypes caused by off-target effects and toxicity as frequently seen in conventional knock-down approachesread more
Citations
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A Systematic Genome-Wide Analysis of Zebrafish Protein-Coding Gene Function
TL;DR: In this paper, the authors describe an active project that aims to identify and phenotype the disruptive mutations in every zebrafish protein-coding gene, using a well-annotated Zebrafish reference genome sequence, high-throughput sequencing and efficient chemical mutagenesis.
Journal ArticleDOI
Zebrafish models of human motor neuron diseases: advantages and limitations.
TL;DR: Zebrafish offer an excellent alternative vertebrate model for the molecular and genetic dissection of MND mechanisms, including the conservation of genome and physiological processes and applicable in vivo tools, including easy imaging, loss or gain of function methods, behavioral tests to examine changes in motor activity, and the ease of simultaneous chemical/drug testing on large numbers of animals.
Journal ArticleDOI
Learning from the past and looking to the future: Emerging perspectives for improving the treatment of psychiatric disorders
TL;DR: The present paper chronicles the discovery and development of psychotropic agents, focusing in particular on their mechanisms of action and therapeutic utility, and how problems faced were eventually overcome.
Journal ArticleDOI
Using the zebrafish model for Alzheimer's disease research
TL;DR: The zebrafish is becoming an increasingly popular model for the investigation of Alzheimer’s disease and will complement studies using other models to help complete the understanding of this disease.
Journal ArticleDOI
Zebrafish: an emerging real-time model system to study Alzheimer’s disease and neurospecific drug discovery
TL;DR: This review appraises the high degree of neurological and behavioural resemblance of zebrafish with humans and further elaborate on the use of recent real-time imaging techniques to obtain vital insights into the neurodegeneration that occurs in AD.
References
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Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
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Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
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TL;DR: It is shown that TDP-43 is the major disease protein in both frontotemporal lobar degeneration with ubiquitin-positive inclusions and amyotrophic lateral sclerosis.
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